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The map label for this gene is cmlA [H]

Identifier: 189353130

GI number: 189353130

Start: 1322807

End: 1324039

Strand: Reverse

Name: cmlA [H]

Synonym: BMULJ_04366

Alternate gene names: 189353130

Gene position: 1324039-1322807 (Counterclockwise)

Preceding gene: 189353131

Following gene: 189353123

Centisome position: 53.54

GC content: 71.86

Gene sequence:

>1233_bases
TTGCAATCGAATCCGTCGAACGGTCCGGCCAGCGGCGCGCCCGCGCGCACGACGCTGATGCTGCTGTGTGCGTTCTCGGT
GCTGCCGCTGAGCCTGTTCCTGCCGTCGCTGCCGGCCATCGCGCGCGAGCTGCGTGCGGACTATGCGCTCGTCGGGCTCG
CGATCGGCGTCTACGCGGCACTCGCCGCATCGCTCGAATTGCTGATGGGGCCGTTGTCGGACCGCTTCGGGCGGCGCCCG
ATCGTGCTGACGAGCGTCGGCGTGTTCACGCTCGGCGCAATCGGCTGCGCGCTGGCGACCGACATCCGCACGTTTCTCGC
GTGCCGGTTGATGCAGGCCGCGATCACGTCGGTGTATCCGGTCTCGATGGCGACGATCCGCGACGCCGGCGGCGGCAGCC
GCGCGGCGAGCCGGATCGGTTATGCGGCGATGGCGGCCGCGTTCGCGCCGATGATCGGCCCGACGCTCGGCGGCGCGCTC
GAACAGGCGGCCGGCTGGCGCGCGAACTTCTGGCTACTCGGCGCCGTCGGCGTCGCACTGTTCGCGTGGTGCGCGTTCGA
TTTCGCCGAAACCCATACGCACCGGTCGCCGACGCTCGCCCGCCAGTTCCGCGCGTATCCGGCGCTGCTCCGCGCGCGCC
GCTTCTGGGCGTACGCGCTCTGCATGGCGTTTTCGACCGGTACGTTCTACGCGTTCCTCGCCGGCGCGCCGCTTGCGGCC
CGCGCGGTGTTCGCGATCGCGCCGGCCGAACTCGGCGTCTGCATGGGGACGATCACGGCCGGTTTCGTATGCGGCAGCTT
TCTCGCGGGCCGCGTCGCGCATCGCTATCCGCTCGCGACGACGATTCTGTATGGCCGCATCGTCGCGTGCGCGGGGCCGC
TGATCGCCCTGGCGCTCCAGGTCGGCGGCGCAACGCATGCGGCTGCGTGGTTCGGGCCGTGCGTGCTGGTCGGGCTCGGC
AACGGGTTCACGAATCCCGGTGCGCATGCGGGCGCGCTGTCGGTGCGACCGGAGCTGGCCGGTAGCGCGTCCGGGCTTGC
CGGCGCGATGACGATCGCGGGCGGCGCTGCGCTGTCGTCGCTGACGAGCGCGGTCGTGACGTCGCGCAATGCCGGCTATG
CACCGCTTGCGATGATGCTGTTGTCCGCGGCGGCGGCGCTGGCCGCCGCGTGCTGTGTGCGGTCGTTCGATGCGCGCGAC
CCGGCCGGCGCGGACGCGATCGCGGTGGAATGA

Upstream 100 bases:

>100_bases
GATCGCGGCTCTGTGTCCGCCGCATCCTCGTCTTCCCGATTCGATCGGGATTTTCTCGTGTTTGCGCACGGCCTCGCGCG
CGTGCGCGGAGGGCATTCGC

Downstream 100 bases:

>100_bases
CTGCGCGCAGCAGGTCACGTCGCACGCCGATTTCGGCGTGAGTGCTTCAAGCGCGCGAAAGACGTAGCCGCGTGCGGTTG
TTGCTTTGGTGGCGCTCGTC

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQSNPSNGPASGAPARTTLMLLCAFSVLPLSLFLPSLPAIARELRADYALVGLAIGVYAALAASLELLMGPLSDRFGRRP
IVLTSVGVFTLGAIGCALATDIRTFLACRLMQAAITSVYPVSMATIRDAGGGSRAASRIGYAAMAAAFAPMIGPTLGGAL
EQAAGWRANFWLLGAVGVALFAWCAFDFAETHTHRSPTLARQFRAYPALLRARRFWAYALCMAFSTGTFYAFLAGAPLAA
RAVFAIAPAELGVCMGTITAGFVCGSFLAGRVAHRYPLATTILYGRIVACAGPLIALALQVGGATHAAAWFGPCVLVGLG
NGFTNPGAHAGALSVRPELAGSASGLAGAMTIAGGAALSSLTSAVVTSRNAGYAPLAMMLLSAAAALAAACCVRSFDARD
PAGADAIAVE

Sequences:

>Translated_410_residues
MQSNPSNGPASGAPARTTLMLLCAFSVLPLSLFLPSLPAIARELRADYALVGLAIGVYAALAASLELLMGPLSDRFGRRP
IVLTSVGVFTLGAIGCALATDIRTFLACRLMQAAITSVYPVSMATIRDAGGGSRAASRIGYAAMAAAFAPMIGPTLGGAL
EQAAGWRANFWLLGAVGVALFAWCAFDFAETHTHRSPTLARQFRAYPALLRARRFWAYALCMAFSTGTFYAFLAGAPLAA
RAVFAIAPAELGVCMGTITAGFVCGSFLAGRVAHRYPLATTILYGRIVACAGPLIALALQVGGATHAAAWFGPCVLVGLG
NGFTNPGAHAGALSVRPELAGSASGLAGAMTIAGGAALSSLTSAVVTSRNAGYAPLAMMLLSAAAALAAACCVRSFDARD
PAGADAIAVE
>Mature_410_residues
MQSNPSNGPASGAPARTTLMLLCAFSVLPLSLFLPSLPAIARELRADYALVGLAIGVYAALAASLELLMGPLSDRFGRRP
IVLTSVGVFTLGAIGCALATDIRTFLACRLMQAAITSVYPVSMATIRDAGGGSRAASRIGYAAMAAAFAPMIGPTLGGAL
EQAAGWRANFWLLGAVGVALFAWCAFDFAETHTHRSPTLARQFRAYPALLRARRFWAYALCMAFSTGTFYAFLAGAPLAA
RAVFAIAPAELGVCMGTITAGFVCGSFLAGRVAHRYPLATTILYGRIVACAGPLIALALQVGGATHAAAWFGPCVLVGLG
NGFTNPGAHAGALSVRPELAGSASGLAGAMTIAGGAALSSLTSAVVTSRNAGYAPLAMMLLSAAAALAAACCVRSFDARD
PAGADAIAVE

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=338, Percent_Identity=26.0355029585799, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082312, Length=347, Percent_Identity=25.9365994236311, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787065, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994889, Length=345, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790794, Length=156, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788509, Length=363, Percent_Identity=26.4462809917355, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319480, Length=302, Percent_Identity=22.8476821192053, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=181, Percent_Identity=32.0441988950276, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=163, Percent_Identity=29.4478527607362, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41657; Mature: 41657

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNPSNGPASGAPARTTLMLLCAFSVLPLSLFLPSLPAIARELRADYALVGLAIGVYAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAASLELLMGPLSDRFGRRPIVLTSVGVFTLGAIGCALATDIRTFLACRLMQAAITSVYP
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSMATIRDAGGGSRAASRIGYAAMAAAFAPMIGPTLGGALEQAAGWRANFWLLGAVGVAL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAWCAFDFAETHTHRSPTLARQFRAYPALLRARRFWAYALCMAFSTGTFYAFLAGAPLAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
RAVFAIAPAELGVCMGTITAGFVCGSFLAGRVAHRYPLATTILYGRIVACAGPLIALALQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VGGATHAAAWFGPCVLVGLGNGFTNPGAHAGALSVRPELAGSASGLAGAMTIAGGAALSS
HCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCEECCHHHCCCCCCCHHHHHHHHHHHHHH
LTSAVVTSRNAGYAPLAMMLLSAAAALAAACCVRSFDARDPAGADAIAVE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure
MQSNPSNGPASGAPARTTLMLLCAFSVLPLSLFLPSLPAIARELRADYALVGLAIGVYAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAASLELLMGPLSDRFGRRPIVLTSVGVFTLGAIGCALATDIRTFLACRLMQAAITSVYP
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSMATIRDAGGGSRAASRIGYAAMAAAFAPMIGPTLGGALEQAAGWRANFWLLGAVGVAL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAWCAFDFAETHTHRSPTLARQFRAYPALLRARRFWAYALCMAFSTGTFYAFLAGAPLAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
RAVFAIAPAELGVCMGTITAGFVCGSFLAGRVAHRYPLATTILYGRIVACAGPLIALALQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VGGATHAAAWFGPCVLVGLGNGFTNPGAHAGALSVRPELAGSASGLAGAMTIAGGAALSS
HCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCEECCHHHCCCCCCCHHHHHHHHHHHHHH
LTSAVVTSRNAGYAPLAMMLLSAAAALAAACCVRSFDARDPAGADAIAVE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]