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The map label for this gene is aspB [H]

Identifier: 189353117

GI number: 189353117

Start: 1301747

End: 1303198

Strand: Direct

Name: aspB [H]

Synonym: BMULJ_04353

Alternate gene names: 189353117

Gene position: 1301747-1303198 (Clockwise)

Preceding gene: 189353116

Following gene: 189353124

Centisome position: 52.63

GC content: 69.9

Gene sequence:

>1452_bases
ATGAAACGCTACGAAACCCTCGCGCATACGATCGCCGACGAAATCCGCCACGGCAACCTCGCGGCCGGCACGCGGCTGCC
TTCGCTGCGACAGATCATCGCGCAGCACGGCGTCAGCCAGTCCACCGTGTTTCGCGCGTACTACCTGCTCGAGCAGTGGG
GGCTGATTCGCGCACGCGAACGTTCGGGGTACTACGTCGCGCCGGGCGCCGTTGCGCCGGGCGCCGCCGCGCGGGGGTCT
GGCGCGTCGGAATCGCGCGCGTCGGACCGCGCCGCATCCACGCGCAAGGTCGACATCAGCGATCTCGTGTTCTCGGTGCT
CGACGCCGCGACGCAGCCCGGCATCGTGCCGCTCGGCTCCGCGTTTCCGTCGCCGCAGCTGTTTCCGCTGCCGCGCGTCG
CGAAGTCGCTCGCGCAGGCCGCGCGGCGCATCAGCCCGTGGAGCACCGTCGTCGATCTGCCGCCCGGCAACGAAGCGCTG
CGCCGGCAGATCGCGCTGCGCTATCTCGCGACCGGCATCGCGCAGCCGATCGACGAGCTGGTCGTCACGAGCGGCGCGCT
CGAAGCGCTGAATCTCTGCCTGATGGCCGTCACGCGGCCGGGCGACGTGGTGGCCGTCGAATCGCCCGGTTTCTACGCAG
CACTGCAGGCGATCGAGCGGCTCGATCTGCGCGCGGTCGAGATTCCGGTCGATCCGCGCACGGGCCTCGATCTCGATGCG
CTCGCGAATGCGCTCGATCGCCACGACGTGCGTGCATGCTGGTTCATGACGAACTTCCAGAATCCGACCGGCGTCACGTT
GCCGATCGAGAAAAAGCGCGCGCTCGTCGAACTGCTCGCCGCGCGCGACGTGCCGCTGATCGAGGACGACGTCTACGGCG
AGCTGCATTTCGGTCCCGACTATCCGCTGCCGGCCCGCGCGTTCGACCGCCACGGACTCGTGATGCATTGCAGTTCGTTC
TCGAAGACGCTCGCGCCCGGCTACCGGATCGGCTGGGCGGCGGCCGGCCGGTTCGCCGAGAAGGTACAGCGGCTGAAGCT
GATGACGACGCTGTCCGCCAGCATTCCCGCGCAGGCCGGCATCGCGAACTACCTCGAATACGGCGGCTACGATCGCCATC
TGCGCAAGCTGCGCGGCGCGCTGCAGGCGCAGCTCGACCGCATGGACGATGCGCTGCGGCGCTGGCTGCCGCCCGGTGTC
GACTGGGTGCGCCCGGAAGGCGGCTATTTCCTGTGGCTCGCGTTCCCCGACGCGATCGACGCGATGGCGCTGCATCGGCA
GGCGATCGCGCGCGGCATCAGCGTCGCGCCGGGGCCGCTGTTTTCCGCCGCGCACGGTTTCGCGCGCTGCGTGCGCGTGA
ACTTCGGCCATCCGTGGAGCGGCGACATCGAACGCGCGATCCGCGTGCTCGGCGAACTCGCCGCCGCATCGGCCGTGCGC
CGGGAGGGTTGA

Upstream 100 bases:

>100_bases
GTCGCTTTCCTTTTCCGCGCACGCATCGGACAATGGCTCGCGCCCGCCGGCGCGGCCTTTTCCTGGGCCCGTTCGGCGGC
ACCGCCGCCCGCCCAGTCCG

Downstream 100 bases:

>100_bases
TCGCGCCGGGCGTCCGCCGCTTCGTTCAAACGAAAAAAACGGGCCGCCCGCAGGCAGCCCGTTCGGTCTCGTCGGCGCCG
GCCGACGCTGTCATGATGCG

Product: aspartate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKRYETLAHTIADEIRHGNLAAGTRLPSLRQIIAQHGVSQSTVFRAYYLLEQWGLIRARERSGYYVAPGAVAPGAAARGS
GASESRASDRAASTRKVDISDLVFSVLDAATQPGIVPLGSAFPSPQLFPLPRVAKSLAQAARRISPWSTVVDLPPGNEAL
RRQIALRYLATGIAQPIDELVVTSGALEALNLCLMAVTRPGDVVAVESPGFYAALQAIERLDLRAVEIPVDPRTGLDLDA
LANALDRHDVRACWFMTNFQNPTGVTLPIEKKRALVELLAARDVPLIEDDVYGELHFGPDYPLPARAFDRHGLVMHCSSF
SKTLAPGYRIGWAAAGRFAEKVQRLKLMTTLSASIPAQAGIANYLEYGGYDRHLRKLRGALQAQLDRMDDALRRWLPPGV
DWVRPEGGYFLWLAFPDAIDAMALHRQAIARGISVAPGPLFSAAHGFARCVRVNFGHPWSGDIERAIRVLGELAAASAVR
REG

Sequences:

>Translated_483_residues
MKRYETLAHTIADEIRHGNLAAGTRLPSLRQIIAQHGVSQSTVFRAYYLLEQWGLIRARERSGYYVAPGAVAPGAAARGS
GASESRASDRAASTRKVDISDLVFSVLDAATQPGIVPLGSAFPSPQLFPLPRVAKSLAQAARRISPWSTVVDLPPGNEAL
RRQIALRYLATGIAQPIDELVVTSGALEALNLCLMAVTRPGDVVAVESPGFYAALQAIERLDLRAVEIPVDPRTGLDLDA
LANALDRHDVRACWFMTNFQNPTGVTLPIEKKRALVELLAARDVPLIEDDVYGELHFGPDYPLPARAFDRHGLVMHCSSF
SKTLAPGYRIGWAAAGRFAEKVQRLKLMTTLSASIPAQAGIANYLEYGGYDRHLRKLRGALQAQLDRMDDALRRWLPPGV
DWVRPEGGYFLWLAFPDAIDAMALHRQAIARGISVAPGPLFSAAHGFARCVRVNFGHPWSGDIERAIRVLGELAAASAVR
REG
>Mature_483_residues
MKRYETLAHTIADEIRHGNLAAGTRLPSLRQIIAQHGVSQSTVFRAYYLLEQWGLIRARERSGYYVAPGAVAPGAAARGS
GASESRASDRAASTRKVDISDLVFSVLDAATQPGIVPLGSAFPSPQLFPLPRVAKSLAQAARRISPWSTVVDLPPGNEAL
RRQIALRYLATGIAQPIDELVVTSGALEALNLCLMAVTRPGDVVAVESPGFYAALQAIERLDLRAVEIPVDPRTGLDLDA
LANALDRHDVRACWFMTNFQNPTGVTLPIEKKRALVELLAARDVPLIEDDVYGELHFGPDYPLPARAFDRHGLVMHCSSF
SKTLAPGYRIGWAAAGRFAEKVQRLKLMTTLSASIPAQAGIANYLEYGGYDRHLRKLRGALQAQLDRMDDALRRWLPPGV
DWVRPEGGYFLWLAFPDAIDAMALHRQAIARGISVAPGPLFSAAHGFARCVRVNFGHPWSGDIERAIRVLGELAAASAVR
REG

Specific function: Unknown

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI7705897, Length=397, Percent_Identity=25.6926952141058, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI33469970, Length=397, Percent_Identity=25.6926952141058, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4507369, Length=175, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787710, Length=474, Percent_Identity=48.7341772151899, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790797, Length=482, Percent_Identity=36.0995850622407, Blast_Score=295, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6321000, Length=354, Percent_Identity=22.316384180791, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356535, Length=365, Percent_Identity=25.2054794520548, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00155 Aminotran_1_2; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 52555; Mature: 52555

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYETLAHTIADEIRHGNLAAGTRLPSLRQIIAQHGVSQSTVFRAYYLLEQWGLIRARE
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEC
RSGYYVAPGAVAPGAAARGSGASESRASDRAASTRKVDISDLVFSVLDAATQPGIVPLGS
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AFPSPQLFPLPRVAKSLAQAARRISPWSTVVDLPPGNEALRRQIALRYLATGIAQPIDEL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVTSGALEALNLCLMAVTRPGDVVAVESPGFYAALQAIERLDLRAVEIPVDPRTGLDLDA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHH
LANALDRHDVRACWFMTNFQNPTGVTLPIEKKRALVELLAARDVPLIEDDVYGELHFGPD
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
YPLPARAFDRHGLVMHCSSFSKTLAPGYRIGWAAAGRFAEKVQRLKLMTTLSASIPAQAG
CCCCCHHHCCCCEEEEECHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IANYLEYGGYDRHLRKLRGALQAQLDRMDDALRRWLPPGVDWVRPEGGYFLWLAFPDAID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHH
AMALHRQAIARGISVAPGPLFSAAHGFARCVRVNFGHPWSGDIERAIRVLGELAAASAVR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
REG
CCC
>Mature Secondary Structure
MKRYETLAHTIADEIRHGNLAAGTRLPSLRQIIAQHGVSQSTVFRAYYLLEQWGLIRARE
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEC
RSGYYVAPGAVAPGAAARGSGASESRASDRAASTRKVDISDLVFSVLDAATQPGIVPLGS
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AFPSPQLFPLPRVAKSLAQAARRISPWSTVVDLPPGNEALRRQIALRYLATGIAQPIDEL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVTSGALEALNLCLMAVTRPGDVVAVESPGFYAALQAIERLDLRAVEIPVDPRTGLDLDA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHH
LANALDRHDVRACWFMTNFQNPTGVTLPIEKKRALVELLAARDVPLIEDDVYGELHFGPD
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
YPLPARAFDRHGLVMHCSSFSKTLAPGYRIGWAAAGRFAEKVQRLKLMTTLSASIPAQAG
CCCCCHHHCCCCEEEEECHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IANYLEYGGYDRHLRKLRGALQAQLDRMDDALRRWLPPGVDWVRPEGGYFLWLAFPDAID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHH
AMALHRQAIARGISVAPGPLFSAAHGFARCVRVNFGHPWSGDIERAIRVLGELAAASAVR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
REG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]