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The map label for this gene is stp [H]

Identifier: 189353102

GI number: 189353102

Start: 1284916

End: 1286304

Strand: Direct

Name: stp [H]

Synonym: BMULJ_04338

Alternate gene names: 189353102

Gene position: 1284916-1286304 (Clockwise)

Preceding gene: 189353098

Following gene: 189353106

Centisome position: 51.95

GC content: 71.49

Gene sequence:

>1389_bases
GTGCCGAACGACGGACATCCTTCTTCGCCCGCGTCGACGCGCGCGACGCATCCCGGCTTCACGCTACTGACCCTCTGTAT
CGCGGTGCTCGTCGCACAGATCGACACGGCCGTCGTGAATCTCGCCACGCGTGCGATCGGCGACTATTTCCACGCAGGTG
TCGGCGCGCTGCAATGGGTCGTCGACAGCTACAACCTCACGTACGCGGTGCTGCTGCTGACCGGCGGATTGCTCGCCGAT
CTTTACGGGCGCCGGCGGATCTTCATCGCGGGCACCGCGCTGTTCACGGTCGCCTCGCTGCTCTGCGCATTCGCGCCGAC
GGTCGGCGTGCTGATCGGCGCGCGTGCGCTCGCCGGCGTCGGCGCGGCGCTGCTGCTGCCGGCCTCGCTTGCGATCGTGC
GCGTGGTATGGCGCGATCCGGTCGAACGCGGCCGCGCGCTCGGCGTATGGGCCGCGTGCAACGGCGTCGCGATGGCAATC
GGGCCGACCGTCGGCGGCGTCCTGATCCGGCACTTCGGCTGGCGCAGCATCTTCTTCGTCGTCGTGCCGCTCAGTGTCGC
CGCGATGCTGTTTGCGATACCTGCCGTGCCCGAATCGTCGGACCGGCGCGGCCGTCACGTCGACGCAGGCGCGCAGGCCG
CCGGCGCGCTCGCGCTCGGCGCGCTCGCCTATGCGGCGATCGTGCTGCGCGATACGCCGGTCATCGCCGCGATCGCCTTC
GCCGTCGCGCTCGTCGCGTTTCGCGTCTTCGTCGCGATCGAGCACCGTCACGGCGCGGCTGCGCTCGTGCCGCTCGATCT
GTTCCGCCTGCACGCATTTCGCGGCGCGATCGCGGCAACGACCGGCATGACGTTCGGCATGTATGGCGTGCTGTTTCTGC
TGCCGCTCACGTGGCAGAGCATCGGGCGACTCGATTCGACGGGCGCGGGCCTCGCGCTGCTACCGATGGCGCTCGTGTTC
GTCGCGGTATCGCCGTGCTCGGGCCGGCTGTCCGAGCAGATCGGACAGCGCGCGACGACGGCCGGCGGCGTGGCGGTGAT
TGCAGCGGGGCTCGGCGTGATCGGCGCGTCGGCGGCGTCGCCGGCATTGATCGGCACCGAGATCGGCCTGGGCCTGACAG
GACTCGGGATGGGGATTGCGACCGGGCCGCTGATGACGGTCGCGGTCGGCGCGGTCGACGCCGCGCGGTCGGGCACGGCC
AGCGCGCTCGTCAATGTCGCGCGGATGGCAGGCGCAACGCTCGGCATCGCGGTGCTCGGCACGCTGTTCGCAGTGCTGCA
CGGCGGTCCGGCCGGGCTGCGCATCGCGATGTTCGCGGGCGCGGCGATTCAGCTCGCCGGCGCGGCGGCAGCGGCCGTCG
GCGTGCGGCGCGAACGCAAGCTCGCGTGA

Upstream 100 bases:

>100_bases
CGCAGCGCGCGATGCGCGACGTTCTGCCCTGCTCCTGACACGACGGTGTCAGCAGCACCGCGCTATGCTGACTCCATTCC
AGTTCGCGGAGCGCTTCATC

Downstream 100 bases:

>100_bases
TGCGGGTCGCGCGGGATCGCAATCGAGATGGGAAAAAAACAGCGCGCTCCCGTCCCTTTGCGGTCACGGTGCGCGCAGAC
CGACGCGGCCGACCGAAGGT

Product: permease of the major facilitator superfamily

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPNDGHPSSPASTRATHPGFTLLTLCIAVLVAQIDTAVVNLATRAIGDYFHAGVGALQWVVDSYNLTYAVLLLTGGLLAD
LYGRRRIFIAGTALFTVASLLCAFAPTVGVLIGARALAGVGAALLLPASLAIVRVVWRDPVERGRALGVWAACNGVAMAI
GPTVGGVLIRHFGWRSIFFVVVPLSVAAMLFAIPAVPESSDRRGRHVDAGAQAAGALALGALAYAAIVLRDTPVIAAIAF
AVALVAFRVFVAIEHRHGAAALVPLDLFRLHAFRGAIAATTGMTFGMYGVLFLLPLTWQSIGRLDSTGAGLALLPMALVF
VAVSPCSGRLSEQIGQRATTAGGVAVIAAGLGVIGASAASPALIGTEIGLGLTGLGMGIATGPLMTVAVGAVDAARSGTA
SALVNVARMAGATLGIAVLGTLFAVLHGGPAGLRIAMFAGAAIQLAGAAAAAVGVRRERKLA

Sequences:

>Translated_462_residues
MPNDGHPSSPASTRATHPGFTLLTLCIAVLVAQIDTAVVNLATRAIGDYFHAGVGALQWVVDSYNLTYAVLLLTGGLLAD
LYGRRRIFIAGTALFTVASLLCAFAPTVGVLIGARALAGVGAALLLPASLAIVRVVWRDPVERGRALGVWAACNGVAMAI
GPTVGGVLIRHFGWRSIFFVVVPLSVAAMLFAIPAVPESSDRRGRHVDAGAQAAGALALGALAYAAIVLRDTPVIAAIAF
AVALVAFRVFVAIEHRHGAAALVPLDLFRLHAFRGAIAATTGMTFGMYGVLFLLPLTWQSIGRLDSTGAGLALLPMALVF
VAVSPCSGRLSEQIGQRATTAGGVAVIAAGLGVIGASAASPALIGTEIGLGLTGLGMGIATGPLMTVAVGAVDAARSGTA
SALVNVARMAGATLGIAVLGTLFAVLHGGPAGLRIAMFAGAAIQLAGAAAAAVGVRRERKLA
>Mature_461_residues
PNDGHPSSPASTRATHPGFTLLTLCIAVLVAQIDTAVVNLATRAIGDYFHAGVGALQWVVDSYNLTYAVLLLTGGLLADL
YGRRRIFIAGTALFTVASLLCAFAPTVGVLIGARALAGVGAALLLPASLAIVRVVWRDPVERGRALGVWAACNGVAMAIG
PTVGGVLIRHFGWRSIFFVVVPLSVAAMLFAIPAVPESSDRRGRHVDAGAQAAGALALGALAYAAIVLRDTPVIAAIAFA
VALVAFRVFVAIEHRHGAAALVPLDLFRLHAFRGAIAATTGMTFGMYGVLFLLPLTWQSIGRLDSTGAGLALLPMALVFV
AVSPCSGRLSEQIGQRATTAGGVAVIAAGLGVIGASAASPALIGTEIGLGLTGLGMGIATGPLMTVAVGAVDAARSGTAS
ALVNVARMAGATLGIAVLGTLFAVLHGGPAGLRIAMFAGAAIQLAGAAAAAVGVRRERKLA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=28.0701754385965, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=28.4313725490196, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789042, Length=413, Percent_Identity=23.9709443099274, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788710, Length=435, Percent_Identity=23.448275862069, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788392, Length=314, Percent_Identity=29.2993630573248, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790146, Length=148, Percent_Identity=28.3783783783784, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=307, Percent_Identity=25.0814332247557, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=183, Percent_Identity=28.4153005464481, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46533; Mature: 46401

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNDGHPSSPASTRATHPGFTLLTLCIAVLVAQIDTAVVNLATRAIGDYFHAGVGALQWV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDSYNLTYAVLLLTGGLLADLYGRRRIFIAGTALFTVASLLCAFAPTVGVLIGARALAGV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAALLLPASLAIVRVVWRDPVERGRALGVWAACNGVAMAIGPTVGGVLIRHFGWRSIFFV
CHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCEEEEECCHHHHHHHHHHCHHHHHHH
VVPLSVAAMLFAIPAVPESSDRRGRHVDAGAQAAGALALGALAYAAIVLRDTPVIAAIAF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AVALVAFRVFVAIEHRHGAAALVPLDLFRLHAFRGAIAATTGMTFGMYGVLFLLPLTWQS
HHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IGRLDSTGAGLALLPMALVFVAVSPCSGRLSEQIGQRATTAGGVAVIAAGLGVIGASAAS
HCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PALIGTEIGLGLTGLGMGIATGPLMTVAVGAVDAARSGTASALVNVARMAGATLGIAVLG
CCEEEHHHCCCHHHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TLFAVLHGGPAGLRIAMFAGAAIQLAGAAAAAVGVRRERKLA
HHHHHHCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNDGHPSSPASTRATHPGFTLLTLCIAVLVAQIDTAVVNLATRAIGDYFHAGVGALQWV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDSYNLTYAVLLLTGGLLADLYGRRRIFIAGTALFTVASLLCAFAPTVGVLIGARALAGV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAALLLPASLAIVRVVWRDPVERGRALGVWAACNGVAMAIGPTVGGVLIRHFGWRSIFFV
CHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCEEEEECCHHHHHHHHHHCHHHHHHH
VVPLSVAAMLFAIPAVPESSDRRGRHVDAGAQAAGALALGALAYAAIVLRDTPVIAAIAF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AVALVAFRVFVAIEHRHGAAALVPLDLFRLHAFRGAIAATTGMTFGMYGVLFLLPLTWQS
HHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IGRLDSTGAGLALLPMALVFVAVSPCSGRLSEQIGQRATTAGGVAVIAAGLGVIGASAAS
HCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PALIGTEIGLGLTGLGMGIATGPLMTVAVGAVDAARSGTASALVNVARMAGATLGIAVLG
CCEEEHHHCCCHHHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TLFAVLHGGPAGLRIAMFAGAAIQLAGAAAAAVGVRRERKLA
HHHHHHCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]