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The map label for this gene is mcbR [H]

Identifier: 189352997

GI number: 189352997

Start: 1160199

End: 1160909

Strand: Reverse

Name: mcbR [H]

Synonym: BMULJ_04231

Alternate gene names: 189352997

Gene position: 1160909-1160199 (Counterclockwise)

Preceding gene: 189352999

Following gene: 189352996

Centisome position: 46.94

GC content: 70.6

Gene sequence:

>711_bases
TTGGCCCCTCGCATCGTCCCGCCCGCACTGAAAGCCATCGAACAGGAAACGATGGCCGACAAGGTCTATCGCCAATTGCG
CGAAGCGCTGATGAGCGGCCGCTTCGCGCCGGGCCAGGCGCTGAGCCTGCGCAGCGTGGCCGAGGCCGTCGGCTCGTCGA
CGATGCCGGTGCGCGCGGCCCTCACGCGGCTGCACGCGGAACGCGCGCTCGTCGACGGCCCGAACCGCGCGCTCGTCGTG
CCGCCGATGACGCTCGACATGCTCGACGAACTGCGCGACGTGCGCATCGCGCTCGAAGGGTGCATCGCCGAACGCGCGTG
CGCACGCATGACCGACGCGCAGATCGCCGCCCTGCGCAAGACCTGCGAGGCCATGCAGGCGCACGCCGAAGCCGGCCGGT
CGCGCGCGTATCTGCAAAGCAATTTCGCGTTTCACAGCGCCATCTACGCGCACGGCGCGAGCGACAGCACGCTCGCGATC
GTCGAAAACCTGTGGATGCGCGTCGGACCATTCCTGAACATGGTCGCGCTCGACATCCCGCACATGCGGCGCTCGATGGA
CGCGCACCGCGCGGTCGTCGATGCGCTCGAGCGGCGCGACGGCGCCGGCGTGCGCGCCGGCATCGCGCTCGACATCGGTG
GTGCCGCGCACGATCTGGCCGCGACGCTGCGTGCCGAGCAGGCGCCGCGCCGCGTGAACGCCAAACCGTAA

Upstream 100 bases:

>100_bases
ACGTTTTCCCCAATCCCTTGCGCCTTGACTACCCCGGCCTCATCGCCGACACTTCCCCCATCGCAAGTGTGATCACAGAT
CAAACCAGGAGAACCGCACC

Downstream 100 bases:

>100_bases
CGCCGCGCGCAACGGCGCGCCGCATCCGACAACATCAACGATGGAGACAACCGCGCATGACCGCTTCCGACCTGCTGAAA
CCCTATGACGGCCTTCGCGT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MAPRIVPPALKAIEQETMADKVYRQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLHAERALVDGPNRALVV
PPMTLDMLDELRDVRIALEGCIAERACARMTDAQIAALRKTCEAMQAHAEAGRSRAYLQSNFAFHSAIYAHGASDSTLAI
VENLWMRVGPFLNMVALDIPHMRRSMDAHRAVVDALERRDGAGVRAGIALDIGGAAHDLAATLRAEQAPRRVNAKP

Sequences:

>Translated_236_residues
MAPRIVPPALKAIEQETMADKVYRQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLHAERALVDGPNRALVV
PPMTLDMLDELRDVRIALEGCIAERACARMTDAQIAALRKTCEAMQAHAEAGRSRAYLQSNFAFHSAIYAHGASDSTLAI
VENLWMRVGPFLNMVALDIPHMRRSMDAHRAVVDALERRDGAGVRAGIALDIGGAAHDLAATLRAEQAPRRVNAKP
>Mature_235_residues
APRIVPPALKAIEQETMADKVYRQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLHAERALVDGPNRALVVP
PMTLDMLDELRDVRIALEGCIAERACARMTDAQIAALRKTCEAMQAHAEAGRSRAYLQSNFAFHSAIYAHGASDSTLAIV
ENLWMRVGPFLNMVALDIPHMRRSMDAHRAVVDALERRDGAGVRAGIALDIGGAAHDLAATLRAEQAPRRVNAKP

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=204, Percent_Identity=28.921568627451, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25535; Mature: 25404

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRIVPPALKAIEQETMADKVYRQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
LTRLHAERALVDGPNRALVVPPMTLDMLDELRDVRIALEGCIAERACARMTDAQIAALRK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCEAMQAHAEAGRSRAYLQSNFAFHSAIYAHGASDSTLAIVENLWMRVGPFLNMVALDIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
HMRRSMDAHRAVVDALERRDGAGVRAGIALDIGGAAHDLAATLRAEQAPRRVNAKP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
APRIVPPALKAIEQETMADKVYRQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
LTRLHAERALVDGPNRALVVPPMTLDMLDELRDVRIALEGCIAERACARMTDAQIAALRK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCEAMQAHAEAGRSRAYLQSNFAFHSAIYAHGASDSTLAIVENLWMRVGPFLNMVALDIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
HMRRSMDAHRAVVDALERRDGAGVRAGIALDIGGAAHDLAATLRAEQAPRRVNAKP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]