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The map label for this gene is hpaI [H]

Identifier: 189352904

GI number: 189352904

Start: 1050728

End: 1051534

Strand: Direct

Name: hpaI [H]

Synonym: BMULJ_04138

Alternate gene names: 189352904

Gene position: 1050728-1051534 (Clockwise)

Preceding gene: 189352903

Following gene: 189352905

Centisome position: 42.49

GC content: 70.01

Gene sequence:

>807_bases
ATGCAGATGCCGACGAATACGTTCAAGGCCGCGCTCGCGCGCGGCGACGCGCAGATCGGGCTGTGGCTCGGTCTCGCCAA
TCCGTACAGTGCCGAAATCGTCGCGGGCGCCGGTTTCGACTGGCTGCTGATCGACGGCGAGCACGCGCCGAATACGGTGC
CGTCGATCGTCGCGCAGCTGCAGGCGATCGCGCCGTATCGGTCGCAGCCGGTCGTGCGCGTGCCGTGGAACGATCCGGTG
ATCGTCAAGCAGGTGCTCGATCTCGGCGCGCAGACGCTGCTCGTGCCGATGGTGCAAAGCGCCGACGAAGCGCGCGCAGC
GGTGGCGGCCACGCGCTATCCGCCGCACGGGATGCGCGGCGTCGGCAGCGCGCTCGCGCGTGCGTCGCGGTGGAATCGCG
TGGGCGACTACCTGCATCGCGCGAACGACGAGATGGCCGTGCTCGTGCAGGTCGAGACGCGCGCGGGGCTCGACGCGATC
GACGCGATTGCGCGCGTCGACGGGGTGGACGGCATGTTCATCGGGCCCGCGGACCTGGCCGCCGATCTCGGGCATCTCGG
CAATCCGGGGCATCCGGACGTGCAGGCCGCGATCGACGGCGCGATCCAGGCGATCAAGGCGGCCGGGAAGGCGCCGGGAA
TTCTCAGCGCGGATGAAGCGGCCGCGCGGCGTTACCTCGAAGCGGGCGCGCTGTTCGTCGCGGTCGGCGTCGATACGACG
TTGCTGGCGAGGAGCGCGGAGCGGTTGGCGGCGCAGTTCAAGGGGAAGGCGGGCGAGGTTGTGAAGAAGGGGGATGGGAC
GTATTGA

Upstream 100 bases:

>100_bases
GGGTCGTTTACACGGCCGTGCGCGGCGCGTCGCGGCGACACGTTCAGTGTCGACTACGGCCCGCTCGGAACGATTCAGTG
CTACTTCGGGTGAACTGACG

Downstream 100 bases:

>100_bases
ACGATGAAGCCGGCTGCGCTCGGAGTATTTGGGGTTCGTTCAAATTTCGTTTATTGCGAATTTCGTTTATTTCGAATAGA
CTGTTGAAATCCTGAAATTC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQMPTNTFKAALARGDAQIGLWLGLANPYSAEIVAGAGFDWLLIDGEHAPNTVPSIVAQLQAIAPYRSQPVVRVPWNDPV
IVKQVLDLGAQTLLVPMVQSADEARAAVAATRYPPHGMRGVGSALARASRWNRVGDYLHRANDEMAVLVQVETRAGLDAI
DAIARVDGVDGMFIGPADLAADLGHLGNPGHPDVQAAIDGAIQAIKAAGKAPGILSADEAAARRYLEAGALFVAVGVDTT
LLARSAERLAAQFKGKAGEVVKKGDGTY

Sequences:

>Translated_268_residues
MQMPTNTFKAALARGDAQIGLWLGLANPYSAEIVAGAGFDWLLIDGEHAPNTVPSIVAQLQAIAPYRSQPVVRVPWNDPV
IVKQVLDLGAQTLLVPMVQSADEARAAVAATRYPPHGMRGVGSALARASRWNRVGDYLHRANDEMAVLVQVETRAGLDAI
DAIARVDGVDGMFIGPADLAADLGHLGNPGHPDVQAAIDGAIQAIKAAGKAPGILSADEAAARRYLEAGALFVAVGVDTT
LLARSAERLAAQFKGKAGEVVKKGDGTY
>Mature_268_residues
MQMPTNTFKAALARGDAQIGLWLGLANPYSAEIVAGAGFDWLLIDGEHAPNTVPSIVAQLQAIAPYRSQPVVRVPWNDPV
IVKQVLDLGAQTLLVPMVQSADEARAAVAATRYPPHGMRGVGSALARASRWNRVGDYLHRANDEMAVLVQVETRAGLDAI
DAIARVDGVDGMFIGPADLAADLGHLGNPGHPDVQAAIDGAIQAIKAAGKAPGILSADEAAARRYLEAGALFVAVGVDTT
LLARSAERLAAQFKGKAGEVVKKGDGTY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=265, Percent_Identity=53.2075471698113, Blast_Score=280, Evalue=6e-77,
Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=52.6104417670683, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: NA

Molecular weight: Translated: 27983; Mature: 27983

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMPTNTFKAALARGDAQIGLWLGLANPYSAEIVAGAGFDWLLIDGEHAPNTVPSIVAQL
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHH
QAIAPYRSQPVVRVPWNDPVIVKQVLDLGAQTLLVPMVQSADEARAAVAATRYPPHGMRG
HHHCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHH
VGSALARASRWNRVGDYLHRANDEMAVLVQVETRAGLDAIDAIARVDGVDGMFIGPADLA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHH
ADLGHLGNPGHPDVQAAIDGAIQAIKAAGKAPGILSADEAAARRYLEAGALFVAVGVDTT
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHH
LLARSAERLAAQFKGKAGEVVKKGDGTY
HHHHHHHHHHHHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MQMPTNTFKAALARGDAQIGLWLGLANPYSAEIVAGAGFDWLLIDGEHAPNTVPSIVAQL
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHH
QAIAPYRSQPVVRVPWNDPVIVKQVLDLGAQTLLVPMVQSADEARAAVAATRYPPHGMRG
HHHCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHH
VGSALARASRWNRVGDYLHRANDEMAVLVQVETRAGLDAIDAIARVDGVDGMFIGPADLA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHH
ADLGHLGNPGHPDVQAAIDGAIQAIKAAGKAPGILSADEAAARRYLEAGALFVAVGVDTT
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHH
LLARSAERLAAQFKGKAGEVVKKGDGTY
HHHHHHHHHHHHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA