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The map label for this gene is hpaH [H]

Identifier: 189352903

GI number: 189352903

Start: 1049917

End: 1050720

Strand: Direct

Name: hpaH [H]

Synonym: BMULJ_04137

Alternate gene names: 189352903

Gene position: 1049917-1050720 (Clockwise)

Preceding gene: 189352902

Following gene: 189352904

Centisome position: 42.45

GC content: 67.16

Gene sequence:

>804_bases
ATGCTCGATACCGCCACCATCGATGCGCTCGCGCAGCGTCTGCACGACGCCGAACGCGAACGCACGCAGATCCGCCAGAT
CTCGCTCGACCATCCGGACATCACGATCGACGACGCGTATGCGATCCAGCGCGCGTGGGTTGCGCTGAAGCTCGCGGAAG
GCCGCACGCTGAAGGGGCACAAGATCGGCCTCACGTCGAAGGCGATGCAGAACACGTCGCAGATCGACGAGCCCGACTAC
GGTGCGCTGCTCGACGACATGTTCTTCGAAGACGGCGGCACGATTCCGACCGATCGCTTCATCGTGCCGCGCGTCGAAGT
CGAACTCGCGTTCGTGCTCGGCAAGCGCCTGAGCGGCCCGAACTGCACGATCTTCGACGTCTACGACGCGGTCCACTACG
TGGTGCCCGCGCTCGAGATCATCGACGCGCGCAGCCAGTCGATCGATCCCGACACGCAGCGTCCGCGCAAGGTGTTCGAC
ACGATCGCCGACAACGCGGCGAACGCGGGCGTCGTGATCGGCGGGCGGCCGGTGAAGCCGCACGATGTCGATCTGCGCTG
GGTCGCGGCCATCATGTCGCGCAACGGCGTGGTCGAGGAAACGGGCGTGGCGGCGGGCGTGCTGAATCACCCGGCGAACG
GCGTCGCGTGGCTCGCGAACCGGCTCGCGCGCTTCGACGTCGCGCTCGAGCGCGGCCAGATCGTGCTCGGCGGGTCGTTT
ACACGGCCGTGCGCGGCGCGTCGCGGCGACACGTTCAGTGTCGACTACGGCCCGCTCGGAACGATTCAGTGCTACTTCGG
GTGA

Upstream 100 bases:

>100_bases
CGCTGTCGATGGAGCTGACCGAGTTCAGCGAAAGCGGCTCGTACAAGCACAACAACATCCACGCCCGCTACAAGCGGGCC
GACTGAATCCGAACCCCATC

Downstream 100 bases:

>100_bases
ACTGACGATGCAGATGCCGACGAATACGTTCAAGGCCGCGCTCGCGCGCGGCGACGCGCAGATCGGGCTGTGGCTCGGTC
TCGCCAATCCGTACAGTGCC

Product: 2-oxo-hept-3-ene-1,7-dioate hydratase

Products: H2O; 2-oxopent-4-enoate [C]

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLDTATIDALAQRLHDAERERTQIRQISLDHPDITIDDAYAIQRAWVALKLAEGRTLKGHKIGLTSKAMQNTSQIDEPDY
GALLDDMFFEDGGTIPTDRFIVPRVEVELAFVLGKRLSGPNCTIFDVYDAVHYVVPALEIIDARSQSIDPDTQRPRKVFD
TIADNAANAGVVIGGRPVKPHDVDLRWVAAIMSRNGVVEETGVAAGVLNHPANGVAWLANRLARFDVALERGQIVLGGSF
TRPCAARRGDTFSVDYGPLGTIQCYFG

Sequences:

>Translated_267_residues
MLDTATIDALAQRLHDAERERTQIRQISLDHPDITIDDAYAIQRAWVALKLAEGRTLKGHKIGLTSKAMQNTSQIDEPDY
GALLDDMFFEDGGTIPTDRFIVPRVEVELAFVLGKRLSGPNCTIFDVYDAVHYVVPALEIIDARSQSIDPDTQRPRKVFD
TIADNAANAGVVIGGRPVKPHDVDLRWVAAIMSRNGVVEETGVAAGVLNHPANGVAWLANRLARFDVALERGQIVLGGSF
TRPCAARRGDTFSVDYGPLGTIQCYFG
>Mature_267_residues
MLDTATIDALAQRLHDAERERTQIRQISLDHPDITIDDAYAIQRAWVALKLAEGRTLKGHKIGLTSKAMQNTSQIDEPDY
GALLDDMFFEDGGTIPTDRFIVPRVEVELAFVLGKRLSGPNCTIFDVYDAVHYVVPALEIIDARSQSIDPDTQRPRKVFD
TIADNAANAGVVIGGRPVKPHDVDLRWVAAIMSRNGVVEETGVAAGVLNHPANGVAWLANRLARFDVALERGQIVLGGSF
TRPCAARRGDTFSVDYGPLGTIQCYFG

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=264, Percent_Identity=34.8484848484849, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 29211; Mature: 29211

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTATIDALAQRLHDAERERTQIRQISLDHPDITIDDAYAIQRAWVALKLAEGRTLKGH
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECHHHHHHHHHEEEEECCCCEECCC
KIGLTSKAMQNTSQIDEPDYGALLDDMFFEDGGTIPTDRFIVPRVEVELAFVLGKRLSGP
EECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHEEEEEEHHHCCCCC
NCTIFDVYDAVHYVVPALEIIDARSQSIDPDTQRPRKVFDTIADNAANAGVVIGGRPVKP
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCC
HDVDLRWVAAIMSRNGVVEETGVAAGVLNHPANGVAWLANRLARFDVALERGQIVLGGSF
CCCCHHHHHHHHHCCCCEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCC
TRPCAARRGDTFSVDYGPLGTIQCYFG
CCCHHHCCCCEEEECCCCCCEEEEECC
>Mature Secondary Structure
MLDTATIDALAQRLHDAERERTQIRQISLDHPDITIDDAYAIQRAWVALKLAEGRTLKGH
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECHHHHHHHHHEEEEECCCCEECCC
KIGLTSKAMQNTSQIDEPDYGALLDDMFFEDGGTIPTDRFIVPRVEVELAFVLGKRLSGP
EECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHEEEEEEHHHCCCCC
NCTIFDVYDAVHYVVPALEIIDARSQSIDPDTQRPRKVFDTIADNAANAGVVIGGRPVKP
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCC
HDVDLRWVAAIMSRNGVVEETGVAAGVLNHPANGVAWLANRLARFDVALERGQIVLGGSF
CCCCHHHHHHHHHCCCCEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCC
TRPCAARRGDTFSVDYGPLGTIQCYFG
CCCHHHCCCCEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-ketovalerate [C]

Specific reaction: 4-hydroxy-2-ketovalerate = H2O + 2-oxopent-4-enoate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]