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The map label for this gene is ansA [H]

Identifier: 189352847

GI number: 189352847

Start: 971799

End: 972788

Strand: Direct

Name: ansA [H]

Synonym: BMULJ_04081

Alternate gene names: 189352847

Gene position: 971799-972788 (Clockwise)

Preceding gene: 189352846

Following gene: 189352848

Centisome position: 39.29

GC content: 72.12

Gene sequence:

>990_bases
ATGACTTCACCCGCGATCGTCGCGATTCACGGCGGCGCAGGCACGATCCTGCGCGATGCAATGGACACCGACACCGAACG
GCAGTACCGCGCGGAGCTGACTGCGATCCTGCAGGCCGCGCAGGCCGTGCTGGCCGAGGGCGGCAGTGCGCTCGACGCGG
TGACGGTCGCCGTGCGCATGCTCGAAGACTGTCCGCTGTTCAATGCGGGACGCGGCGCGGTCTATACGGCCGAAGGCAAG
CACGAACTCGACGCGGCGATCATGGACGGCGCGACGCGAGCGGCCGGCGCGATCTGCTGCGCGACGCGCGTGCGCAACCC
GGTACTCGCCGCGCGGCGCGTGATGGAAGCGAGCGAGCACGTGCTGTTTGCCGGCGCGGGCGCCGATGCGTTCGCGGCCG
CGCAAGGGCTCGAACTCGTCGAGCCGGGCTACTTCGATACCGAGGCGCGTCATGCGCAGTGGCTCAACGCACGCGCGGCG
GCCGGCATGATGCTCGACCACGATGCGGCGACGTTCGCGTTCGGCGCGCCGCGCCGCGCGGAGCCGCTCGATCCGGACCG
CAAGCACGGCACGGTCGGCGCGGTGGCGCGCGATCTGCACGGGCACGTCGCCGCGGCGACCTCGACCGGCGGCATCACGA
ACAAGCAGCCGGGACGCGTCGGCGACTCGCCGATCATCGGCGCGGGCTGCTACGCGGACGACGCGACCTGCGCGGTGTCC
GCCACGGGCACCGGCGAGATGTTCATCCGGCTCGCGGCCGCGCACGACGTGGCGGCGCAGATCGCCTATCGCGGCGCGAC
GCTCGCGCAGGCCGCGCACGACGTCGTGATGAACCAGCTGCCGCGGATCGCGGGCCGCGGCGGCATCATCGCCGTCGACG
CGTTCGGCAACGTCGCGATGCCGTTCAACACCGAAGGGATGTATCGCGGTCACGCGCGCGTCGGCGCGGCGCCCGTCGTC
GGCATCTATCGCGACGACGATGCGAGCTGA

Upstream 100 bases:

>100_bases
AATCCGCGGCCGAGATGACCGAATTCGTGCTGCCGTGGCTCGTGCAGCCGCAAGCGGCCGTTCCCGCTCCGAACCTTTCC
TTTCCCCGCCCGGACCCATC

Downstream 100 bases:

>100_bases
TGCCGCCGTGTTCGAGGAGACCCGCATGAAACCGAGTCCGTCCGGTCTGGTGCTGCCCGAGCAGCGCGTCGTCGCCGTCG
ACGATCTGTCGGTGATGTTC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTSPAIVAIHGGAGTILRDAMDTDTERQYRAELTAILQAAQAVLAEGGSALDAVTVAVRMLEDCPLFNAGRGAVYTAEGK
HELDAAIMDGATRAAGAICCATRVRNPVLAARRVMEASEHVLFAGAGADAFAAAQGLELVEPGYFDTEARHAQWLNARAA
AGMMLDHDAATFAFGAPRRAEPLDPDRKHGTVGAVARDLHGHVAAATSTGGITNKQPGRVGDSPIIGAGCYADDATCAVS
ATGTGEMFIRLAAAHDVAAQIAYRGATLAQAAHDVVMNQLPRIAGRGGIIAVDAFGNVAMPFNTEGMYRGHARVGAAPVV
GIYRDDDAS

Sequences:

>Translated_329_residues
MTSPAIVAIHGGAGTILRDAMDTDTERQYRAELTAILQAAQAVLAEGGSALDAVTVAVRMLEDCPLFNAGRGAVYTAEGK
HELDAAIMDGATRAAGAICCATRVRNPVLAARRVMEASEHVLFAGAGADAFAAAQGLELVEPGYFDTEARHAQWLNARAA
AGMMLDHDAATFAFGAPRRAEPLDPDRKHGTVGAVARDLHGHVAAATSTGGITNKQPGRVGDSPIIGAGCYADDATCAVS
ATGTGEMFIRLAAAHDVAAQIAYRGATLAQAAHDVVMNQLPRIAGRGGIIAVDAFGNVAMPFNTEGMYRGHARVGAAPVV
GIYRDDDAS
>Mature_328_residues
TSPAIVAIHGGAGTILRDAMDTDTERQYRAELTAILQAAQAVLAEGGSALDAVTVAVRMLEDCPLFNAGRGAVYTAEGKH
ELDAAIMDGATRAAGAICCATRVRNPVLAARRVMEASEHVLFAGAGADAFAAAQGLELVEPGYFDTEARHAQWLNARAAA
GMMLDHDAATFAFGAPRRAEPLDPDRKHGTVGAVARDLHGHVAAATSTGGITNKQPGRVGDSPIIGAGCYADDATCAVSA
TGTGEMFIRLAAAHDVAAQIAYRGATLAQAAHDVVMNQLPRIAGRGGIIAVDAFGNVAMPFNTEGMYRGHARVGAAPVVG
IYRDDDAS

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=324, Percent_Identity=38.5802469135802, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI145275200, Length=324, Percent_Identity=38.5802469135802, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI98991769, Length=307, Percent_Identity=29.6416938110749, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI285002251, Length=252, Percent_Identity=36.1111111111111, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI285002253, Length=252, Percent_Identity=33.7301587301587, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=55.7632398753894, Blast_Score=343, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI17569239, Length=251, Percent_Identity=25.4980079681275, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI18921183, Length=315, Percent_Identity=35.5555555555556, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=32.7137546468402, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24652135, Length=235, Percent_Identity=34.468085106383, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI221330102, Length=235, Percent_Identity=34.468085106383, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24655553, Length=219, Percent_Identity=30.5936073059361, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24657358, Length=203, Percent_Identity=29.064039408867, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33873; Mature: 33742

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAIVAIHGGAGTILRDAMDTDTERQYRAELTAILQAAQAVLAEGGSALDAVTVAVRM
CCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVYTAEGKHELDAAIMDGATRAAGAICCATRVRNPVLAARRVMEASEH
HHHCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VLFAGAGADAFAAAQGLELVEPGYFDTEARHAQWLNARAAAGMMLDHDAATFAFGAPRRA
EEEEECCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCCC
EPLDPDRKHGTVGAVARDLHGHVAAATSTGGITNKQPGRVGDSPIIGAGCYADDATCAVS
CCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEE
ATGTGEMFIRLAAAHDVAAQIAYRGATLAQAAHDVVMNQLPRIAGRGGIIAVDAFGNVAM
ECCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEC
PFNTEGMYRGHARVGAAPVVGIYRDDDAS
CCCCCCCEECCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TSPAIVAIHGGAGTILRDAMDTDTERQYRAELTAILQAAQAVLAEGGSALDAVTVAVRM
CCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVYTAEGKHELDAAIMDGATRAAGAICCATRVRNPVLAARRVMEASEH
HHHCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VLFAGAGADAFAAAQGLELVEPGYFDTEARHAQWLNARAAAGMMLDHDAATFAFGAPRRA
EEEEECCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCCC
EPLDPDRKHGTVGAVARDLHGHVAAATSTGGITNKQPGRVGDSPIIGAGCYADDATCAVS
CCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEE
ATGTGEMFIRLAAAHDVAAQIAYRGATLAQAAHDVVMNQLPRIAGRGGIIAVDAFGNVAM
ECCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEC
PFNTEGMYRGHARVGAAPVVGIYRDDDAS
CCCCCCCEECCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]