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The map label for this gene is cobS [H]

Identifier: 189352758

GI number: 189352758

Start: 869912

End: 870898

Strand: Direct

Name: cobS [H]

Synonym: BMULJ_03990

Alternate gene names: 189352758

Gene position: 869912-870898 (Clockwise)

Preceding gene: 189352756

Following gene: 189352759

Centisome position: 35.17

GC content: 67.38

Gene sequence:

>987_bases
ATGGAAGCGGTAGCGAGCAAGCCGGACTGCATGGTGTCGGTCCGCGAGCGGTTCGGCATCGATTCGGACCTGACGGTGCC
CGCGTTCAGCGTGCGCGACGATCACGTGCCGGACGTCGACGACGCGTATCGATTCAATCCCGACGTGACGCTCGCGATTC
TCGCCGGCTTCGCGCGCAACCGGCGCGTGCTCGTGCAAGGCATGCACGGCACCGGCAAGTCGACGCATATCGAGCAGGTC
GCCGCGCGGCTGAACTGGCCGTGCGTGCGCGTGAACCTCGACGGCCATATCAGCCGGCTCGACCTCGTCGGCAAGGACGC
GATCGTCGTGCGCGACGACGTGCAGGTGACCGAGTTCCAGGAGGGCATCGTGCCGTGGGCGCTGCAGCGGCCGGTCGCGC
TCATCTTCGACGAATACGACGCGGGGCGGCCCGACGTGATGTTCGTGATCCAGCGAATTCTCGAGCGCGACGGCAAGTTC
ACGCTGCTCGACCAGAATCGCGTGATTCATCCGCATCCTTCGTTTCGGCTCTTCGCGACCGCGAACACGGTCGGGCTCGG
CAATCTGAACGGGCTGTATCACGGCACGCAGATGCTCAATCATGCGCAGATGGACCGCTGGAACGTGGTCGCGACGCTCG
ACTACCTGCCGCGCGACGAGGAGATCGGCATCGTGCGCGCGCGCGTGCCGGCCCTCGCGGACGAAGCGGGCCGCACGCTG
CTCGAATCGATGGTGAGCCTCGCCGAGCTCACGCGCAACGGTTTCGCGACCGGCGACGTGTCGACGCTGATGTCGCCGCG
CACCGTGATCGACTGGGCCGAGAACTGCCAGATCTTCCGCGATCCGGCGCTCGCGTTCCGCCTCACGTTCCTGAACAAGT
GCGACGACGCGGAGCGGCCGATCGTCGCCGAGTACTACCAGCGCTGCTTCGGCGACGAGCTCGCGATCGGCGCGCGGGTC
GGCAGCGAGCCGGGAGCCGCGCGGTGA

Upstream 100 bases:

>100_bases
GGCCCGCATTTCACGCAACGGAACGGATCGTTCCGCAAACGGGGCGACGCGCATCGCATCGGCGCGCGTCCGGCCCGCAA
CGACAGAGGAGCGCAACGCA

Downstream 100 bases:

>100_bases
GCGCCGAGCGCGACGCGGCGCGGCGCGCGCTCGCGCACGAGACGCTCGGCGCGGCCGCCGTGCGCGCGATGACGCGCGAC
GCGACGCTGCACTACCGCGC

Product: cobalamin biosynthesis protein

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobS [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEAVASKPDCMVSVRERFGIDSDLTVPAFSVRDDHVPDVDDAYRFNPDVTLAILAGFARNRRVLVQGMHGTGKSTHIEQV
AARLNWPCVRVNLDGHISRLDLVGKDAIVVRDDVQVTEFQEGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKF
TLLDQNRVIHPHPSFRLFATANTVGLGNLNGLYHGTQMLNHAQMDRWNVVATLDYLPRDEEIGIVRARVPALADEAGRTL
LESMVSLAELTRNGFATGDVSTLMSPRTVIDWAENCQIFRDPALAFRLTFLNKCDDAERPIVAEYYQRCFGDELAIGARV
GSEPGAAR

Sequences:

>Translated_328_residues
MEAVASKPDCMVSVRERFGIDSDLTVPAFSVRDDHVPDVDDAYRFNPDVTLAILAGFARNRRVLVQGMHGTGKSTHIEQV
AARLNWPCVRVNLDGHISRLDLVGKDAIVVRDDVQVTEFQEGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKF
TLLDQNRVIHPHPSFRLFATANTVGLGNLNGLYHGTQMLNHAQMDRWNVVATLDYLPRDEEIGIVRARVPALADEAGRTL
LESMVSLAELTRNGFATGDVSTLMSPRTVIDWAENCQIFRDPALAFRLTFLNKCDDAERPIVAEYYQRCFGDELAIGARV
GSEPGAAR
>Mature_328_residues
MEAVASKPDCMVSVRERFGIDSDLTVPAFSVRDDHVPDVDDAYRFNPDVTLAILAGFARNRRVLVQGMHGTGKSTHIEQV
AARLNWPCVRVNLDGHISRLDLVGKDAIVVRDDVQVTEFQEGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKF
TLLDQNRVIHPHPSFRLFATANTVGLGNLNGLYHGTQMLNHAQMDRWNVVATLDYLPRDEEIGIVRARVPALADEAGRTL
LESMVSLAELTRNGFATGDVSTLMSPRTVIDWAENCQIFRDPALAFRLTFLNKCDDAERPIVAEYYQRCFGDELAIGARV
GSEPGAAR

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG0714

COG function: function code R; MoxR-like ATPases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24415404, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323135, Length=202, Percent_Identity=30.6930693069307, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI161076562, Length=191, Percent_Identity=27.2251308900524, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161076560, Length=191, Percent_Identity=27.2251308900524, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011704
- InterPro:   IPR006537 [H]

Pfam domain/function: PF07728 AAA_5 [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 36587; Mature: 36587

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVASKPDCMVSVRERFGIDSDLTVPAFSVRDDHVPDVDDAYRFNPDVTLAILAGFARN
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEECCCEEEEEEHHHHCC
RRVLVQGMHGTGKSTHIEQVAARLNWPCVRVNLDGHISRLDLVGKDAIVVRDDVQVTEFQ
CEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCCHHHHH
EGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKFTLLDQNRVIHPHPSFRLFAT
CCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCEEEEEE
ANTVGLGNLNGLYHGTQMLNHAQMDRWNVVATLDYLPRDEEIGIVRARVPALADEAGRTL
ECEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
LESMVSLAELTRNGFATGDVSTLMSPRTVIDWAENCQIFRDPALAFRLTFLNKCDDAERP
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCEEEECCHHEEEEHHHHCCCCCCCC
IVAEYYQRCFGDELAIGARVGSEPGAAR
HHHHHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MEAVASKPDCMVSVRERFGIDSDLTVPAFSVRDDHVPDVDDAYRFNPDVTLAILAGFARN
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEECCCEEEEEEHHHHCC
RRVLVQGMHGTGKSTHIEQVAARLNWPCVRVNLDGHISRLDLVGKDAIVVRDDVQVTEFQ
CEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCCHHHHH
EGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKFTLLDQNRVIHPHPSFRLFAT
CCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCEEEEEE
ANTVGLGNLNGLYHGTQMLNHAQMDRWNVVATLDYLPRDEEIGIVRARVPALADEAGRTL
ECEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
LESMVSLAELTRNGFATGDVSTLMSPRTVIDWAENCQIFRDPALAFRLTFLNKCDDAERP
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCEEEECCHHEEEEHHHHCCCCCCCC
IVAEYYQRCFGDELAIGARVGSEPGAAR
HHHHHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]