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The map label for this gene is yhdJ [H]

Identifier: 189352659

GI number: 189352659

Start: 748755

End: 749606

Strand: Direct

Name: yhdJ [H]

Synonym: BMULJ_03890

Alternate gene names: 189352659

Gene position: 748755-749606 (Clockwise)

Preceding gene: 189352658

Following gene: 189352660

Centisome position: 30.28

GC content: 66.08

Gene sequence:

>852_bases
ATGCGCGAACTGATCGAAGAGCCGGGCGGCGGTGCCGCGGCCGACGAACCGGCGGCGCAGGCCGCCGCCGTCGCGCCGCG
CGCGCTGCCGCCCGGGATCGAACTGCACAACCGCGATTTCCTGACCGAGGCGGCACGGCTGCCGGACGCGTCGATCGACC
TGATCGTCGCCGATCCGCCGTACGGGCTCGGCAAGGACTACGGCAACGACTCGGACAAGCGCTCGGGCGACGCGTTTCTC
GAATGGACGCGCGAATGGCTCGAACTCGCGATTCCGAAGCTCAAGCCGAGCGGCTCGATGTACGTGTTCTGCACGTGGCA
GTACGCGCCGGAGATCTTCAGTTTCCTGAAGACGAAGCTCACGATGATCAACGAGATCATCTGGGACCGCCGCGTGCCGA
GCATGGGCGGCACGACGCGCCGGTTCACGTCGGTGCACGACAACATCGGCTTTTTCGCAGTCTCGAAAGCGTACTACTTC
GATCTCGATCCGGTTCGCATCCCTTACGACGCCGATACGAAGAAGGCCCGCTCGCGCAAGCTGTTCGAAGGCAGCAAGTG
GCTGGAAATGGGCTACAACCCGAAGGACGTCTGGTCGGTCTCGCGCCTGCATCGGCAGCACGCGGAGCGCGTCGATCATC
CGACCCAGAAGCCGCTCGAAATCATCGAGCGGATGGTGCTCGCGAGCTGCCCGCCGGGCGGCCGCGTGCTCGATCCGTTC
ATGGGCAGCGGCACGACCGCGGTGGCCTGCGCACGGCAGGGGCGCGACTTCGTCGGCTACGAGATCAACGAAAGTTACTG
CGCGATCGCGCGCGAGCGCGTGCATGCGCTCGCTGCGCAGGCGTGTGCATGA

Upstream 100 bases:

>100_bases
TTGCCGAAGGACAAGATCCTGCTCGTCAATCTGTCGGGCCGCGGCGACAAGGACATGCATACGGTCGCCGAGCGATCGGG
CATCACGCTCTGACGGTCCG

Downstream 100 bases:

>100_bases
CGCACGCCGCCGCATCGCCGGTCGCACACTTTGCACGGACCCGGCCGGGGCGCTGACGCGCCGCGTCCGGATCGACACAG
GAAAATTGCCATGTCGCGTA

Product: putative adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRELIEEPGGGAAADEPAAQAAAVAPRALPPGIELHNRDFLTEAARLPDASIDLIVADPPYGLGKDYGNDSDKRSGDAFL
EWTREWLELAIPKLKPSGSMYVFCTWQYAPEIFSFLKTKLTMINEIIWDRRVPSMGGTTRRFTSVHDNIGFFAVSKAYYF
DLDPVRIPYDADTKKARSRKLFEGSKWLEMGYNPKDVWSVSRLHRQHAERVDHPTQKPLEIIERMVLASCPPGGRVLDPF
MGSGTTAVACARQGRDFVGYEINESYCAIARERVHALAAQACA

Sequences:

>Translated_283_residues
MRELIEEPGGGAAADEPAAQAAAVAPRALPPGIELHNRDFLTEAARLPDASIDLIVADPPYGLGKDYGNDSDKRSGDAFL
EWTREWLELAIPKLKPSGSMYVFCTWQYAPEIFSFLKTKLTMINEIIWDRRVPSMGGTTRRFTSVHDNIGFFAVSKAYYF
DLDPVRIPYDADTKKARSRKLFEGSKWLEMGYNPKDVWSVSRLHRQHAERVDHPTQKPLEIIERMVLASCPPGGRVLDPF
MGSGTTAVACARQGRDFVGYEINESYCAIARERVHALAAQACA
>Mature_283_residues
MRELIEEPGGGAAADEPAAQAAAVAPRALPPGIELHNRDFLTEAARLPDASIDLIVADPPYGLGKDYGNDSDKRSGDAFL
EWTREWLELAIPKLKPSGSMYVFCTWQYAPEIFSFLKTKLTMINEIIWDRRVPSMGGTTRRFTSVHDNIGFFAVSKAYYF
DLDPVRIPYDADTKKARSRKLFEGSKWLEMGYNPKDVWSVSRLHRQHAERVDHPTQKPLEIIERMVLASCPPGGRVLDPF
MGSGTTAVACARQGRDFVGYEINESYCAIARERVHALAAQACA

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=241, Percent_Identity=31.9502074688797, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31582; Mature: 31582

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00092 N6_MTASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELIEEPGGGAAADEPAAQAAAVAPRALPPGIELHNRDFLTEAARLPDASIDLIVADPP
CCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCC
YGLGKDYGNDSDKRSGDAFLEWTREWLELAIPKLKPSGSMYVFCTWQYAPEIFSFLKTKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHH
TMINEIIWDRRVPSMGGTTRRFTSVHDNIGFFAVSKAYYFDLDPVRIPYDADTKKARSRK
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECEEEECCCCEECCCCCCHHHHHHHH
LFEGSKWLEMGYNPKDVWSVSRLHRQHAERVDHPTQKPLEIIERMVLASCPPGGRVLDPF
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHH
MGSGTTAVACARQGRDFVGYEINESYCAIARERVHALAAQACA
CCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRELIEEPGGGAAADEPAAQAAAVAPRALPPGIELHNRDFLTEAARLPDASIDLIVADPP
CCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCC
YGLGKDYGNDSDKRSGDAFLEWTREWLELAIPKLKPSGSMYVFCTWQYAPEIFSFLKTKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHH
TMINEIIWDRRVPSMGGTTRRFTSVHDNIGFFAVSKAYYFDLDPVRIPYDADTKKARSRK
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECEEEECCCCEECCCCCCHHHHHHHH
LFEGSKWLEMGYNPKDVWSVSRLHRQHAERVDHPTQKPLEIIERMVLASCPPGGRVLDPF
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHH
MGSGTTAVACARQGRDFVGYEINESYCAIARERVHALAAQACA
CCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]