Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 189352658

GI number: 189352658

Start: 747554

End: 748747

Strand: Direct

Name: trpB [H]

Synonym: BMULJ_03889

Alternate gene names: 189352658

Gene position: 747554-748747 (Clockwise)

Preceding gene: 189352657

Following gene: 189352659

Centisome position: 30.23

GC content: 66.33

Gene sequence:

>1194_bases
ATGTACAACCTTCCTGACGATCGCGGCCACTTCGGCCCGTATGGCGGCGTGTTCGTCGCCGAGACGCTGATTCACGCGCT
GGACGAGCTGCGCGGCGCGTACGCGAAATACCAGAACGACCCCGATTTCGTCGCCGAATTCCAGCGCGAACTGAAGCACT
TCGTCGGCCGTCCGTCGCCGATCTATCACGCGCAGCGCTGGAGCGACACGCTCGGCGGCGCGCAGATCTATCTGAAGCGC
GAAGATTTGAACCACACGGGCGCGCACAAGATCAACAACGTGATCGGCCAGGCGCTGCTTGCGAAGCGCATGGGCAAGAA
GCGCGTAATCGCCGAGACGGGCGCCGGCCAGCACGGCGTCGCCACCGCGACGATCTGCGCGCGCTTCGGGATGGAGTGCG
TCGTCTACATGGGCGCCGAGGACGTGCGCCGCCAGGCCGCCAACGTGTACCGGATGAAGCTGCTCGGCGCGACCGTCGTG
CCGGTCGAATCGGGTTCGCGCACGCTGAAGGACGCGCTGAACGAGGCGATGCGCGACTGGGTCACGAACATCGAGAACAC
GTTCTACATCATCGGCACGGTCGCGGGCCCGCATCCGTACCCGATGATGGTGCGCGACTTCCAGCGCGTGATCGGCGACG
AATGCAAGGTGCAGATGCCCGAACTCGCCGGGCGCCAGCCCGACGCGGTGATCGCCTGCGTCGGCGGCGGCTCGAACGCG
ATGGGGATCTTCTATCCGTACATCGACGACGCGTCGGTGCAGCTGATCGGCGTCGAAGCCGCGGGCGACGGCCTCGACAC
CGGCCATCACGCAGCCTCGCTGATCGCCGGCAGCCCGGGCGTGCTGCACGGCAACCGCACCTATCTGCTGCAGGACGACA
ACGGCCAGATCATCGAGACGCATTCGGTGTCGGCCGGCCTCGACTATCCGGGCGTCGGTCCCGAGCACGCATGGCTGAAG
GACAGCGGTCGCGCGCAGTACGTCGGGATCACCGACGAAGAGGCGCTGAAGGCGTTCCACGACTGCTGCCGGATCGAGGG
CATCATCCCCGCGCTCGAGTCGAGCCACGCGATCGCGTATGGCGTGAAGCTCGCGCCGACGTTGCCGAAGGACAAGATCC
TGCTCGTCAATCTGTCGGGCCGCGGCGACAAGGACATGCATACGGTCGCCGAGCGATCGGGCATCACGCTCTGA

Upstream 100 bases:

>100_bases
GCGGCGTTCGTGCGCGCGGTGCGCGACGCGGACGCCCGGTGATGCCAGCCGGTGCGACTTCCCACAGCGGGGCGCACCGA
CCGATCGAGAGTGACACATC

Downstream 100 bases:

>100_bases
CGGTCCGATGCGCGAACTGATCGAAGAGCCGGGCGGCGGTGCCGCGGCCGACGAACCGGCGGCGCAGGCCGCCGCCGTCG
CGCCGCGCGCGCTGCCGCCC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYNLPDDRGHFGPYGGVFVAETLIHALDELRGAYAKYQNDPDFVAEFQRELKHFVGRPSPIYHAQRWSDTLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATICARFGMECVVYMGAEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNIENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQMPELAGRQPDAVIACVGGGSNA
MGIFYPYIDDASVQLIGVEAAGDGLDTGHHAASLIAGSPGVLHGNRTYLLQDDNGQIIETHSVSAGLDYPGVGPEHAWLK
DSGRAQYVGITDEEALKAFHDCCRIEGIIPALESSHAIAYGVKLAPTLPKDKILLVNLSGRGDKDMHTVAERSGITL

Sequences:

>Translated_397_residues
MYNLPDDRGHFGPYGGVFVAETLIHALDELRGAYAKYQNDPDFVAEFQRELKHFVGRPSPIYHAQRWSDTLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATICARFGMECVVYMGAEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNIENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQMPELAGRQPDAVIACVGGGSNA
MGIFYPYIDDASVQLIGVEAAGDGLDTGHHAASLIAGSPGVLHGNRTYLLQDDNGQIIETHSVSAGLDYPGVGPEHAWLK
DSGRAQYVGITDEEALKAFHDCCRIEGIIPALESSHAIAYGVKLAPTLPKDKILLVNLSGRGDKDMHTVAERSGITL
>Mature_397_residues
MYNLPDDRGHFGPYGGVFVAETLIHALDELRGAYAKYQNDPDFVAEFQRELKHFVGRPSPIYHAQRWSDTLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATICARFGMECVVYMGAEDVRRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNIENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQMPELAGRQPDAVIACVGGGSNA
MGIFYPYIDDASVQLIGVEAAGDGLDTGHHAASLIAGSPGVLHGNRTYLLQDDNGQIIETHSVSAGLDYPGVGPEHAWLK
DSGRAQYVGITDEEALKAFHDCCRIEGIIPALESSHAIAYGVKLAPTLPKDKILLVNLSGRGDKDMHTVAERSGITL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=55.9055118110236, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321412, Length=382, Percent_Identity=58.1151832460733, Blast_Score=468, Evalue=1e-132,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43064; Mature: 43064

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLPDDRGHFGPYGGVFVAETLIHALDELRGAYAKYQNDPDFVAEFQRELKHFVGRPSP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
IYHAQRWSDTLGGAQIYLKREDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGV
CCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
ATATICARFGMECVVYMGAEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNIENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQMPELAGRQPDAVIACVGGGSNA
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECHHHCCCCCCEEEEEECCCCCC
MGIFYPYIDDASVQLIGVEAAGDGLDTGHHAASLIAGSPGVLHGNRTYLLQDDNGQIIET
EEEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCEEECCEEEEEECCCCCEEEE
HSVSAGLDYPGVGPEHAWLKDSGRAQYVGITDEEALKAFHDCCRIEGIIPALESSHAIAY
CCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCCEEEE
GVKLAPTLPKDKILLVNLSGRGDKDMHTVAERSGITL
EEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MYNLPDDRGHFGPYGGVFVAETLIHALDELRGAYAKYQNDPDFVAEFQRELKHFVGRPSP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
IYHAQRWSDTLGGAQIYLKREDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGV
CCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
ATATICARFGMECVVYMGAEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNIENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQMPELAGRQPDAVIACVGGGSNA
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECHHHCCCCCCEEEEEECCCCCC
MGIFYPYIDDASVQLIGVEAAGDGLDTGHHAASLIAGSPGVLHGNRTYLLQDDNGQIIET
EEEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCEEECCEEEEEECCCCCEEEE
HSVSAGLDYPGVGPEHAWLKDSGRAQYVGITDEEALKAFHDCCRIEGIIPALESSHAIAY
CCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCCEEEE
GVKLAPTLPKDKILLVNLSGRGDKDMHTVAERSGITL
EEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]