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The map label for this gene is truA [H]

Identifier: 189352656

GI number: 189352656

Start: 745979

End: 746791

Strand: Direct

Name: truA [H]

Synonym: BMULJ_03887

Alternate gene names: 189352656

Gene position: 745979-746791 (Clockwise)

Preceding gene: 189352655

Following gene: 189352657

Centisome position: 30.16

GC content: 69.62

Gene sequence:

>813_bases
ATGCGGATCGCGCTGGGCATACAGTACGACGGCGCCGCGTTCTGCGGCTGGCAGGCGCAGCCGCACGGCAACACGGTGCA
GGACGCGCTCGAGCGCGCGCTCGCGGAGTTCGCGCGCGTACCGCTGCATACGACGGTCGCGGGGCGCACCGACACGGGCG
TGCACGGGCTCGGACAGGTCGTGCATTTCGACACCGAGCTCGACCGCGCGGACTTCTCGTGGGTCCGCGGCACGAACGCG
TTTTTGCCGCCGACGGTCGCCGTGCAATGGGCGAAGTCGATGCCGGACGCGTTCCACGCGCGCTTCTCCGCGTTCGAGCG
CACCTATTACTACGCGTTGTACGTGCATCCGGTGCGTTCGCCGATGCTCGACGGCCGCGCGGGCTGGATCCACACGCCGC
TCGACGACGACGCGATGCGCGCCGCCGCCGCGCATCTGCTCGGCGAGCACGACTTTTCGGCGTTCCGCTCGTCGGAATGC
CAGTCGAAGACGCCGGTGAAGCATTTGTACGAGATCGACATCCGCCGCGTCGGACACTTCCTGCATTTCCGGTTCCGCGC
GAACGCGTTCCTGCATCATATGGTGCGCAACCTGATGGGCTGCCTCGTCGCGGTCGGGCGCGGCCGCTATCCGGCCGACT
GGGTCGCCGACGTGCTGGCCGGCCGCGACCGCAACCGCGCAGCGCCCACCTTCATGGCCGACGGGCTGTATCTCGCGCAC
GTCGGCTACCCGGCGGAATTCGCCGTCCCGCCCGCGCAGCTCGGCAGCGTGCCGTGGAGCAGCGTCTGGGCCGACCTGGA
CCCGCAACCATGA

Upstream 100 bases:

>100_bases
CCGGCGCACGCACGCTGCTGCAGGAAGTGGTCGACGCGAACGATGCGGCGACGCGCGACGATGCGCGCGCGATGCTCGCG
AAACTGTCGGAAGACGCGTG

Downstream 100 bases:

>100_bases
ACGATCCCGTTTCCTCCCGCTCGCTCGCCGAGTCAGCCGAATCCGCCGTGCCGCCGCGCACGCGCATCAAGCTGTGCGGG
CTGTCGCGCCCGGACGACGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRIALGIQYDGAAFCGWQAQPHGNTVQDALERALAEFARVPLHTTVAGRTDTGVHGLGQVVHFDTELDRADFSWVRGTNA
FLPPTVAVQWAKSMPDAFHARFSAFERTYYYALYVHPVRSPMLDGRAGWIHTPLDDDAMRAAAAHLLGEHDFSAFRSSEC
QSKTPVKHLYEIDIRRVGHFLHFRFRANAFLHHMVRNLMGCLVAVGRGRYPADWVADVLAGRDRNRAAPTFMADGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDPQP

Sequences:

>Translated_270_residues
MRIALGIQYDGAAFCGWQAQPHGNTVQDALERALAEFARVPLHTTVAGRTDTGVHGLGQVVHFDTELDRADFSWVRGTNA
FLPPTVAVQWAKSMPDAFHARFSAFERTYYYALYVHPVRSPMLDGRAGWIHTPLDDDAMRAAAAHLLGEHDFSAFRSSEC
QSKTPVKHLYEIDIRRVGHFLHFRFRANAFLHHMVRNLMGCLVAVGRGRYPADWVADVLAGRDRNRAAPTFMADGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDPQP
>Mature_270_residues
MRIALGIQYDGAAFCGWQAQPHGNTVQDALERALAEFARVPLHTTVAGRTDTGVHGLGQVVHFDTELDRADFSWVRGTNA
FLPPTVAVQWAKSMPDAFHARFSAFERTYYYALYVHPVRSPMLDGRAGWIHTPLDDDAMRAAAAHLLGEHDFSAFRSSEC
QSKTPVKHLYEIDIRRVGHFLHFRFRANAFLHHMVRNLMGCLVAVGRGRYPADWVADVLAGRDRNRAAPTFMADGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDPQP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=33.0855018587361, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=47.244094488189, Blast_Score=246, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30094; Mature: 30094

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIQYDGAAFCGWQAQPHGNTVQDALERALAEFARVPLHTTVAGRTDTGVHGLGQV
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHE
VHFDTELDRADFSWVRGTNAFLPPTVAVQWAKSMPDAFHARFSAFERTYYYALYVHPVRS
EEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCC
PMLDGRAGWIHTPLDDDAMRAAAAHLLGEHDFSAFRSSECQSKTPVKHLYEIDIRRVGHF
CCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LHFRFRANAFLHHMVRNLMGCLVAVGRGRYPADWVADVLAGRDRNRAAPTFMADGLYLAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
VGYPAEFAVPPAQLGSVPWSSVWADLDPQP
ECCCHHCCCCHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MRIALGIQYDGAAFCGWQAQPHGNTVQDALERALAEFARVPLHTTVAGRTDTGVHGLGQV
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHE
VHFDTELDRADFSWVRGTNAFLPPTVAVQWAKSMPDAFHARFSAFERTYYYALYVHPVRS
EEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCC
PMLDGRAGWIHTPLDDDAMRAAAAHLLGEHDFSAFRSSECQSKTPVKHLYEIDIRRVGHF
CCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LHFRFRANAFLHHMVRNLMGCLVAVGRGRYPADWVADVLAGRDRNRAAPTFMADGLYLAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
VGYPAEFAVPPAQLGSVPWSSVWADLDPQP
ECCCHHCCCCHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA