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The map label for this gene is emrB [H]

Identifier: 189352600

GI number: 189352600

Start: 681296

End: 682885

Strand: Reverse

Name: emrB [H]

Synonym: BMULJ_03831

Alternate gene names: 189352600

Gene position: 682885-681296 (Counterclockwise)

Preceding gene: 189352601

Following gene: 189352599

Centisome position: 27.61

GC content: 66.6

Gene sequence:

>1590_bases
ATGGCTGAGCCGACTGCCACCGTGCCGCCCGCGCCGCCGCATGAAGGCCGCGCGAGCGTGACCGACTGGATCGCCGTCGC
GGCCGGCGCGCTCGGCGCGTTGATGGCGACGCTCGACATCTCGATCACGAACTCCGCGCTGCCGCAGATCCAGGGCGAAA
TCGGCGCGACGGGCACCGAAGGCACGTGGATCTCGACCGGCTACCTGATGTCCGAGATCGTGATGATCCCGCTCGCCGCG
TGGCTCACGCGCGTATTCGGGCTGCGCAATTTCCTGCTCGTGAATTCCGCGCTGTTCATCGCGTTCTCGATGATGTGCGG
CTGGTCGCATTCGCTCGCGATGATGGTCGTCGGCCGGATCGGCCAGGGCTTCACCGGCGGCGCACTGATTCCGACGGCGC
AGACGATCATCCGCACGCGGCTGCCGCTGTCGCAACTGCCGGTCGGGATGACGCTGTTCGGCCTGATCGTGCTGCTCGGC
CCGTTGTTCGGCCCCGTGCTCGGCGGCTGGCTCGCGGAGAACGTGAGCTGGAGCTGGTGCTTCTTTCTGAATCTGCCGGT
ATGCCTGCTGCTGATGGCGCTGCTCGTGTTCGGGCTGCCGTCGGACCGGCCGCAGTGGAGCACGTTCTTCAACGCCGACT
GGCTCGGCATCGCGGGCCTCGCGATCGGATTGAGCTCGCTGACGGTCGTGCTCGAGGAAGGCCAGCGCGAACGCTGGTTC
GAATCGCAGATGATCGTCACGCTGAGCTACGTGTCGCTCGCGGGGATGATCCTGATCGCGCTGTCGCAACGTTTCGCGAA
GCGGCCGATCATGCGGCTGTCGCTGATGCGCAATCCGCGCTACGCGAGCGTGATCGTGATCGTGTCGGCGGTCGGCGCGG
GGCTGTACGGCGTGTCGTATCTGCTGCCGCAGTTCCTCGCGATCGTCGCGGGCTACAACGCGGAACAGGCCGGCTCGATC
ATGCTGCTCTCCGGGCTGCCCGCCTTTCTCGTGATGCCGATCCTGCCGCGGCTGCTCGGCAAGGTCGACTTCCGCATCCT
CGTGATCTCCGGCCTGCTGCTGTTCTGCCTGAGCTGCATGCTCGACATCGATCTGACCGCGCAGAGCGTCGGCCACGATT
TCGTCTGGTCGCAGCTGATTCGCGGCGTCGCGCAGATGCTCGCGATGATGCCGCTGAACCAGGCGTCGATGGCGGCCGTC
GCGCGCGAGGACTCGGGCGACGCGGCGGGCCTCTACAACATGGCGCGCAATCTCGGCGGCTCGATCGGACTCGCGATCAT
CGGCACGGTGATCGATCGGCGCACGACGTTTCATACGGCCGCGCTGCGCGAGTCGGTCACCGCCAATGCGTCGATCGGGC
AGGAGCGATTGTCGGCGTACGCGGCGAACTGGTTTGCGCAGACCGGCGACCTCGCCTATTCGAAAATGCGCGCGCTCGGT
CAGCTCGCGCAGCAAATCCAGATACAGGCCGTCGTGATGACCTATTCCGAAACCTTCTACCTGCTGGGACTCGCGCTGCT
CGCCTGCGTGCCGCTCGCGCTGCTGCTGAAGACGCCGCGCACGCCGCAGCCGATGTCGTCCGGCCACTGA

Upstream 100 bases:

>100_bases
ATCCGCATCGCACCGAACGAGCGCGCGCGGCACATGCTGCTGCCGGGGCTGTCGGTGACGGTCGACGTCGACACGCGCTC
GGCGCGCAACGAGAACCGTC

Downstream 100 bases:

>100_bases
ACCCGCGATGAAGCCTCTCTTCCCGCCCCGCCGCCCCGCGCTCGCGCTCTGCACGGCCGCGTGCCTGCTCGCCGGCTGCA
CGGTCGGCCCCGACTACCGC

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAEPTATVPPAPPHEGRASVTDWIAVAAGALGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIPLAA
WLTRVFGLRNFLLVNSALFIAFSMMCGWSHSLAMMVVGRIGQGFTGGALIPTAQTIIRTRLPLSQLPVGMTLFGLIVLLG
PLFGPVLGGWLAENVSWSWCFFLNLPVCLLLMALLVFGLPSDRPQWSTFFNADWLGIAGLAIGLSSLTVVLEEGQRERWF
ESQMIVTLSYVSLAGMILIALSQRFAKRPIMRLSLMRNPRYASVIVIVSAVGAGLYGVSYLLPQFLAIVAGYNAEQAGSI
MLLSGLPAFLVMPILPRLLGKVDFRILVISGLLLFCLSCMLDIDLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMAAV
AREDSGDAAGLYNMARNLGGSIGLAIIGTVIDRRTTFHTAALRESVTANASIGQERLSAYAANWFAQTGDLAYSKMRALG
QLAQQIQIQAVVMTYSETFYLLGLALLACVPLALLLKTPRTPQPMSSGH

Sequences:

>Translated_529_residues
MAEPTATVPPAPPHEGRASVTDWIAVAAGALGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIPLAA
WLTRVFGLRNFLLVNSALFIAFSMMCGWSHSLAMMVVGRIGQGFTGGALIPTAQTIIRTRLPLSQLPVGMTLFGLIVLLG
PLFGPVLGGWLAENVSWSWCFFLNLPVCLLLMALLVFGLPSDRPQWSTFFNADWLGIAGLAIGLSSLTVVLEEGQRERWF
ESQMIVTLSYVSLAGMILIALSQRFAKRPIMRLSLMRNPRYASVIVIVSAVGAGLYGVSYLLPQFLAIVAGYNAEQAGSI
MLLSGLPAFLVMPILPRLLGKVDFRILVISGLLLFCLSCMLDIDLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMAAV
AREDSGDAAGLYNMARNLGGSIGLAIIGTVIDRRTTFHTAALRESVTANASIGQERLSAYAANWFAQTGDLAYSKMRALG
QLAQQIQIQAVVMTYSETFYLLGLALLACVPLALLLKTPRTPQPMSSGH
>Mature_528_residues
AEPTATVPPAPPHEGRASVTDWIAVAAGALGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIPLAAW
LTRVFGLRNFLLVNSALFIAFSMMCGWSHSLAMMVVGRIGQGFTGGALIPTAQTIIRTRLPLSQLPVGMTLFGLIVLLGP
LFGPVLGGWLAENVSWSWCFFLNLPVCLLLMALLVFGLPSDRPQWSTFFNADWLGIAGLAIGLSSLTVVLEEGQRERWFE
SQMIVTLSYVSLAGMILIALSQRFAKRPIMRLSLMRNPRYASVIVIVSAVGAGLYGVSYLLPQFLAIVAGYNAEQAGSIM
LLSGLPAFLVMPILPRLLGKVDFRILVISGLLLFCLSCMLDIDLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMAAVA
REDSGDAAGLYNMARNLGGSIGLAIIGTVIDRRTTFHTAALRESVTANASIGQERLSAYAANWFAQTGDLAYSKMRALGQ
LAQQIQIQAVVMTYSETFYLLGLALLACVPLALLLKTPRTPQPMSSGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=512, Percent_Identity=30.6640625, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1788710, Length=522, Percent_Identity=26.6283524904215, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=25.7281553398058, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1790195, Length=419, Percent_Identity=26.0143198090692, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=143, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=355, Percent_Identity=23.3802816901408, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=272, Percent_Identity=24.6323529411765, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=165, Percent_Identity=23.6363636363636, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56746; Mature: 56615

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTATVPPAPPHEGRASVTDWIAVAAGALGALMATLDISITNSALPQIQGEIGATGTE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCCCCCC
GTWISTGYLMSEIVMIPLAAWLTRVFGLRNFLLVNSALFIAFSMMCGWSHSLAMMVVGRI
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GQGFTGGALIPTAQTIIRTRLPLSQLPVGMTLFGLIVLLGPLFGPVLGGWLAENVSWSWC
CCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEH
FFLNLPVCLLLMALLVFGLPSDRPQWSTFFNADWLGIAGLAIGLSSLTVVLEEGQRERWF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ESQMIVTLSYVSLAGMILIALSQRFAKRPIMRLSLMRNPRYASVIVIVSAVGAGLYGVSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLPQFLAIVAGYNAEQAGSIMLLSGLPAFLVMPILPRLLGKVDFRILVISGLLLFCLSCM
HHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIDLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMAAVAREDSGDAAGLYNMARNLGG
HCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
SIGLAIIGTVIDRRTTFHTAALRESVTANASIGQERLSAYAANWFAQTGDLAYSKMRALG
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
QLAQQIQIQAVVMTYSETFYLLGLALLACVPLALLLKTPRTPQPMSSGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AEPTATVPPAPPHEGRASVTDWIAVAAGALGALMATLDISITNSALPQIQGEIGATGTE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCCCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]