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The map label for this gene is ispA [H]

Identifier: 189352476

GI number: 189352476

Start: 525764

End: 526648

Strand: Direct

Name: ispA [H]

Synonym: BMULJ_03695

Alternate gene names: 189352476

Gene position: 525764-526648 (Clockwise)

Preceding gene: 189352475

Following gene: 189352477

Centisome position: 21.26

GC content: 69.83

Gene sequence:

>885_bases
ATGACATTCGAACAATGGATGCGGTCCGTGCTAGACCGCGTCGAGGACGCACTCGGCCACTATCTGCCGGCTGACACCGT
GGTGCCCGCACGACTCCACGAAGCGATGCGCTATGCGGTCCTCGGCGGCGGCAAGCGCGTTCGCCCGCTGCTGTGCCATG
CGGCAGGCGAACTGACCGGTGCGACGGAGGCGGCGCGCAATGCGGCGGCGGCGGCGCTGGAGATGATCCACGTCTACTCG
CTCGTGCACGACGACATGCCGTGCATGGACGACGACGCGCTGCGTCGCGGCAAGCCGACCGTGCACGTGCAGTACGACGA
GCCCACCGCACTGCTGGTCGGCGACGCGCTGCAGTCGCAGGCGTTCGTCGCGCTGACCGACGCGGCTGCCCTGTCGCCGG
TGCAGCAGGCGGCGCTCGTGCGCGAGCTCGCGCTCGCGAGCGGCTCGCTCGGGATGGCGGGCGGCCAGGCGATCGATCTC
GCGAGCGTCGGCATCAAGCTGACGCGCGAGCAGCTCGAAACGATGCACCGGATGAAGACGGGCGCGCTGCTGCGCGCGGC
GGTGCGGATGGGCGCGCTGGCCGGCGAGACGCCGTCGGCCGACACAATGGCTGCACTCGACGTCTATGCGGGGGCCGTGG
GTCTGGCCTTCCAGGTCGTCGACGACATTCTCGACGTCACGACCGATTCGGCGACGCTCGGCAAGACGGCCGGCAAGGAC
GCGGCGAACGACAAGCCGACCTACGTATCGATCCTCGGCCTCGATGCGTCGCGCGAGCTCGCCGCGCAGTTGCGCGCCGA
AGCGCACGATGCGCTGAAACCGTTCGGCGCACGCGCGCAGCGTCTCGCCGAACTTGCCGACCTGGTAGTGAACCGGGTCA
GCTGA

Upstream 100 bases:

>100_bases
TTTGCCAACAGCAGCTCGAGAAAGTGGAGCAGCAGGTTCGCGTGCTCGACGGCGCGACGCTGAAGCCGGCGGCCGCCACG
GACGGCGAAGACGACGATCT

Downstream 100 bases:

>100_bases
CGCGAAAGCCCGCCGCCGCGCACACGCTACGGGTGCGTGCAACAGAATGCGGGCGCGTTTGATTTCCTACAATGGAACGA
CGATGTACGACTTGCTGAAA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTFEQWMRSVLDRVEDALGHYLPADTVVPARLHEAMRYAVLGGGKRVRPLLCHAAGELTGATEAARNAAAAALEMIHVYS
LVHDDMPCMDDDALRRGKPTVHVQYDEPTALLVGDALQSQAFVALTDAAALSPVQQAALVRELALASGSLGMAGGQAIDL
ASVGIKLTREQLETMHRMKTGALLRAAVRMGALAGETPSADTMAALDVYAGAVGLAFQVVDDILDVTTDSATLGKTAGKD
AANDKPTYVSILGLDASRELAAQLRAEAHDALKPFGARAQRLAELADLVVNRVS

Sequences:

>Translated_294_residues
MTFEQWMRSVLDRVEDALGHYLPADTVVPARLHEAMRYAVLGGGKRVRPLLCHAAGELTGATEAARNAAAAALEMIHVYS
LVHDDMPCMDDDALRRGKPTVHVQYDEPTALLVGDALQSQAFVALTDAAALSPVQQAALVRELALASGSLGMAGGQAIDL
ASVGIKLTREQLETMHRMKTGALLRAAVRMGALAGETPSADTMAALDVYAGAVGLAFQVVDDILDVTTDSATLGKTAGKD
AANDKPTYVSILGLDASRELAAQLRAEAHDALKPFGARAQRLAELADLVVNRVS
>Mature_293_residues
TFEQWMRSVLDRVEDALGHYLPADTVVPARLHEAMRYAVLGGGKRVRPLLCHAAGELTGATEAARNAAAAALEMIHVYSL
VHDDMPCMDDDALRRGKPTVHVQYDEPTALLVGDALQSQAFVALTDAAALSPVQQAALVRELALASGSLGMAGGQAIDLA
SVGIKLTREQLETMHRMKTGALLRAAVRMGALAGETPSADTMAALDVYAGAVGLAFQVVDDILDVTTDSATLGKTAGKDA
ANDKPTYVSILGLDASRELAAQLRAEAHDALKPFGARAQRLAELADLVVNRVS

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=220, Percent_Identity=30.4545454545455, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786623, Length=297, Percent_Identity=44.4444444444444, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789578, Length=212, Percent_Identity=33.4905660377358, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 30962; Mature: 30831

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEQWMRSVLDRVEDALGHYLPADTVVPARLHEAMRYAVLGGGKRVRPLLCHAAGELTG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
ATEAARNAAAAALEMIHVYSLVHDDMPCMDDDALRRGKPTVHVQYDEPTALLVGDALQSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCEEEECHHHHCC
AFVALTDAAALSPVQQAALVRELALASGSLGMAGGQAIDLASVGIKLTREQLETMHRMKT
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
GALLRAAVRMGALAGETPSADTMAALDVYAGAVGLAFQVVDDILDVTTDSATLGKTAGKD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
AANDKPTYVSILGLDASRELAAQLRAEAHDALKPFGARAQRLAELADLVVNRVS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFEQWMRSVLDRVEDALGHYLPADTVVPARLHEAMRYAVLGGGKRVRPLLCHAAGELTG
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
ATEAARNAAAAALEMIHVYSLVHDDMPCMDDDALRRGKPTVHVQYDEPTALLVGDALQSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCEEEECHHHHCC
AFVALTDAAALSPVQQAALVRELALASGSLGMAGGQAIDLASVGIKLTREQLETMHRMKT
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
GALLRAAVRMGALAGETPSADTMAALDVYAGAVGLAFQVVDDILDVTTDSATLGKTAGKD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
AANDKPTYVSILGLDASRELAAQLRAEAHDALKPFGARAQRLAELADLVVNRVS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]