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The map label for this gene is hisJ [H]

Identifier: 189352394

GI number: 189352394

Start: 452392

End: 453174

Strand: Reverse

Name: hisJ [H]

Synonym: BMULJ_03613

Alternate gene names: 189352394

Gene position: 453174-452392 (Counterclockwise)

Preceding gene: 189352397

Following gene: 189352390

Centisome position: 18.32

GC content: 65.64

Gene sequence:

>783_bases
ATGAAGAGACTGATTGCCGCCGTTTCGATCGCCCTGCTCGCGGTATCCGCAGGCCCCGCCGTCGCGAAGGACTGGACCAC
GATCCGCTTCGGCACCGACGCCAGCTACGCGCCGTTCGAGTCGAAGGCGCCCGACGGCAAGCTCGTCGGCTTCGACATCG
ATCTCGGCAACGAGATCTGCGCGCGCCTGAAGGCGAAGTGCGTCTGGCTCGAGAACGATTTCGACGGGATGATCCCGGCG
CTGAAGGCGAAGAAGTTCGACGCGGTGCTGTCGTCGATGTCGATCACGCCGCAGCGCGCGGAGCAGATCGGCTTCACGAC
GAAGATCTACAACCAGCCGACGCGGCTCGTCGTCAAGAAGGGCTCGCCGCTGCTGCCGACGGCCGAATCGCTGAAGGGCA
AGTCGATCGGCGTCGAACAGGGGACGACGCAGGAAGCGTATGCGAAGGCGTACTGGGCGAAGCAAGGCGCGAACGTCGTG
TCGTACCAGAACCAGGACGGCGTCTACGCGGACCTGACGGCCGGCCGCCTCGACGCCGCGCTGCAGGACGAGGTGCAGGC
CGCGATCGGCTTTCTGAAGTCGCCGCGCGGTGCGGGCTACCAGTTCGTCGGGCCCGAGCTCGTCGACGAGAAGGTGCTCG
GCATCGGCGCGGGCATCGGCCTGCGCAAGGAGGACACCGACCTGAAGGCGAAGATCGACCGCGCGATCCTCGACATGGTC
AAGGACGGCACGTACAAGCGGCTCGCGTCGAAGTACTTCGACTTCGATATCTACGGCGGCTGA

Upstream 100 bases:

>100_bases
TTCTGCGTTAATGGCCGCACGGATCATGCGCCGTCAACGCGGCGCCGGTCCGCGGAAACCGTGTCGCCCCGAACCGGAAG
GACCCCGTGAAGGAACCATC

Downstream 100 bases:

>100_bases
CGCACGGCCGCCGCCGCGCGCGTCGCGACGGCGTCAGTACGCGAAGAGCGGGCGCCTCGGCATCGGCGCCCGCGCGCACA
TCCCCTTGCGGAGCCGGCTC

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKRLIAAVSIALLAVSAGPAVAKDWTTIRFGTDASYAPFESKAPDGKLVGFDIDLGNEICARLKAKCVWLENDFDGMIPA
LKAKKFDAVLSSMSITPQRAEQIGFTTKIYNQPTRLVVKKGSPLLPTAESLKGKSIGVEQGTTQEAYAKAYWAKQGANVV
SYQNQDGVYADLTAGRLDAALQDEVQAAIGFLKSPRGAGYQFVGPELVDEKVLGIGAGIGLRKEDTDLKAKIDRAILDMV
KDGTYKRLASKYFDFDIYGG

Sequences:

>Translated_260_residues
MKRLIAAVSIALLAVSAGPAVAKDWTTIRFGTDASYAPFESKAPDGKLVGFDIDLGNEICARLKAKCVWLENDFDGMIPA
LKAKKFDAVLSSMSITPQRAEQIGFTTKIYNQPTRLVVKKGSPLLPTAESLKGKSIGVEQGTTQEAYAKAYWAKQGANVV
SYQNQDGVYADLTAGRLDAALQDEVQAAIGFLKSPRGAGYQFVGPELVDEKVLGIGAGIGLRKEDTDLKAKIDRAILDMV
KDGTYKRLASKYFDFDIYGG
>Mature_260_residues
MKRLIAAVSIALLAVSAGPAVAKDWTTIRFGTDASYAPFESKAPDGKLVGFDIDLGNEICARLKAKCVWLENDFDGMIPA
LKAKKFDAVLSSMSITPQRAEQIGFTTKIYNQPTRLVVKKGSPLLPTAESLKGKSIGVEQGTTQEAYAKAYWAKQGANVV
SYQNQDGVYADLTAGRLDAALQDEVQAAIGFLKSPRGAGYQFVGPELVDEKVLGIGAGIGLRKEDTDLKAKIDRAILDMV
KDGTYKRLASKYFDFDIYGG

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=57.2649572649573, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=52.8957528957529, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=34.3873517786561, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787085, Length=254, Percent_Identity=33.8582677165354, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1788228, Length=259, Percent_Identity=30.5019305019305, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=31.2252964426877, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28039; Mature: 28039

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLIAAVSIALLAVSAGPAVAKDWTTIRFGTDASYAPFESKAPDGKLVGFDIDLGNEIC
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
ARLKAKCVWLENDFDGMIPALKAKKFDAVLSSMSITPQRAEQIGFTTKIYNQPTRLVVKK
HHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEEEC
GSPLLPTAESLKGKSIGVEQGTTQEAYAKAYWAKQGANVVSYQNQDGVYADLTAGRLDAA
CCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHH
LQDEVQAAIGFLKSPRGAGYQFVGPELVDEKVLGIGAGIGLRKEDTDLKAKIDRAILDMV
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
KDGTYKRLASKYFDFDIYGG
CCCHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MKRLIAAVSIALLAVSAGPAVAKDWTTIRFGTDASYAPFESKAPDGKLVGFDIDLGNEIC
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
ARLKAKCVWLENDFDGMIPALKAKKFDAVLSSMSITPQRAEQIGFTTKIYNQPTRLVVKK
HHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEEEC
GSPLLPTAESLKGKSIGVEQGTTQEAYAKAYWAKQGANVVSYQNQDGVYADLTAGRLDAA
CCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHH
LQDEVQAAIGFLKSPRGAGYQFVGPELVDEKVLGIGAGIGLRKEDTDLKAKIDRAILDMV
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
KDGTYKRLASKYFDFDIYGG
CCCHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]