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The map label for this gene is gcvA [H]

Identifier: 189352303

GI number: 189352303

Start: 351926

End: 352837

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_03522

Alternate gene names: 189352303

Gene position: 352837-351926 (Counterclockwise)

Preceding gene: 189352304

Following gene: 189352302

Centisome position: 14.27

GC content: 66.78

Gene sequence:

>912_bases
ATGCGCATCACGCCCCTCCCGCCCCTTCAGTGCCTCGTCGCGTTCGAAGCCGCCGTCCGGCACGCAAGCTTCACGCGCGC
GGCCGCCGAACTGCACCTGACCCAAAGCGCGATCAGTCGTCAGATCCAGCAGCTCGAGGAATTCCTCGGCCGCTCGCTGT
TCGTCCGCGAACACCGTTCGCTGCGGCTGACGATCGCCGGCGAGCAATACGCGGCGCAGGTGCATCACCTGCTCACGCAA
TGCGCGGAAGCGACGCACGACGTGATGAAGCCGTACGGCGACCTCGAGCTGACGATCGCCTGCTCGTCCGGTGTGGCGGT
GCTGTGGCTCACGCCGCGGCTACCCGAGTTTCGTGCCGCGTATCCGAACATCAAGCTGCGGCTGATCGTGCGCGACGGGC
TCGCGTCGATGTCGCCGGCGGAGTTCGATGTGGGCGTCTACTACGTGCGTCAGCAGGCGCCCGCCGAATACACCGCGCGC
CGCCTGTTCGACGAGGAAGTGTTTCCCGTCTGCGCGCCGGGCTATCTGGCCGGACGCACGCTCGACGCAGCCGATCTCGC
GAACGAAACGCTGCTGCGCCTCGAGGACGGGCAGCGTCAATGGATGTCGTGGTCCGAATGGTTCGACCAGAACGACATCG
ACCGGCAGAAGGCGCAGCCGCAGGACATCACGATCAACTCGTATCCGCAGATCGTGCAGATGACGATACTCGGTCAGGGC
GTGTCGCTCGGCTGGCGCTACATGATCGACGCGTGCATCGAGGCCGGGGTGCTCGTGCGCGTCACCGACGCATCGGCGAG
CCACGGCGGCGGCTATTACGTGATCTCGCCGAACGACCGCGCGCAGAACCAGGCCGCGCGCCTGTTCTCGCGCTGGCTGT
TCGAACAGGCGGAGCGGCAAACCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGACGCGCGGCTTACTCGTCCAGGGGAATGAGGCGCGGCCGGTTTGCTCTGACGATCGTCCCCGTATCATGTCGGCAGTG
CCACCGATTCCCCTGCCGCC

Downstream 100 bases:

>100_bases
GCCGGCGTGAGCGCGATGCATGCGTCCAGCGCATGCGTGCATGCGAATCTTTCGTTTCAGAAAAAAATCGCGCTGTCCTT
AGCATGAGGTTCACGCGGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRITPLPPLQCLVAFEAAVRHASFTRAAAELHLTQSAISRQIQQLEEFLGRSLFVREHRSLRLTIAGEQYAAQVHHLLTQ
CAEATHDVMKPYGDLELTIACSSGVAVLWLTPRLPEFRAAYPNIKLRLIVRDGLASMSPAEFDVGVYYVRQQAPAEYTAR
RLFDEEVFPVCAPGYLAGRTLDAADLANETLLRLEDGQRQWMSWSEWFDQNDIDRQKAQPQDITINSYPQIVQMTILGQG
VSLGWRYMIDACIEAGVLVRVTDASASHGGGYYVISPNDRAQNQAARLFSRWLFEQAERQTAA

Sequences:

>Translated_303_residues
MRITPLPPLQCLVAFEAAVRHASFTRAAAELHLTQSAISRQIQQLEEFLGRSLFVREHRSLRLTIAGEQYAAQVHHLLTQ
CAEATHDVMKPYGDLELTIACSSGVAVLWLTPRLPEFRAAYPNIKLRLIVRDGLASMSPAEFDVGVYYVRQQAPAEYTAR
RLFDEEVFPVCAPGYLAGRTLDAADLANETLLRLEDGQRQWMSWSEWFDQNDIDRQKAQPQDITINSYPQIVQMTILGQG
VSLGWRYMIDACIEAGVLVRVTDASASHGGGYYVISPNDRAQNQAARLFSRWLFEQAERQTAA
>Mature_303_residues
MRITPLPPLQCLVAFEAAVRHASFTRAAAELHLTQSAISRQIQQLEEFLGRSLFVREHRSLRLTIAGEQYAAQVHHLLTQ
CAEATHDVMKPYGDLELTIACSSGVAVLWLTPRLPEFRAAYPNIKLRLIVRDGLASMSPAEFDVGVYYVRQQAPAEYTAR
RLFDEEVFPVCAPGYLAGRTLDAADLANETLLRLEDGQRQWMSWSEWFDQNDIDRQKAQPQDITINSYPQIVQMTILGQG
VSLGWRYMIDACIEAGVLVRVTDASASHGGGYYVISPNDRAQNQAARLFSRWLFEQAERQTAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=34.6938775510204, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=30, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786448, Length=304, Percent_Identity=30.5921052631579, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=302, Percent_Identity=28.476821192053, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=38.9221556886228, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=27.906976744186, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=26.2975778546713, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=237, Percent_Identity=30.379746835443, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=174, Percent_Identity=28.1609195402299, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=25.7839721254355, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=143, Percent_Identity=30.0699300699301, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=251, Percent_Identity=29.4820717131474, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34085; Mature: 34085

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITPLPPLQCLVAFEAAVRHASFTRAAAELHLTQSAISRQIQQLEEFLGRSLFVREHRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCE
LRLTIAGEQYAAQVHHLLTQCAEATHDVMKPYGDLELTIACSSGVAVLWLTPRLPEFRAA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCHHHHC
YPNIKLRLIVRDGLASMSPAEFDVGVYYVRQQAPAEYTARRLFDEEVFPVCAPGYLAGRT
CCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCC
LDAADLANETLLRLEDGQRQWMSWSEWFDQNDIDRQKAQPQDITINSYPQIVQMTILGQG
CCHHHHCCHHEEEECCCHHHHCCHHHHCCCCCCCHHHCCCCCEEECCCCCEEEEEEECCC
VSLGWRYMIDACIEAGVLVRVTDASASHGGGYYVISPNDRAQNQAARLFSRWLFEQAERQ
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TAA
CCC
>Mature Secondary Structure
MRITPLPPLQCLVAFEAAVRHASFTRAAAELHLTQSAISRQIQQLEEFLGRSLFVREHRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCE
LRLTIAGEQYAAQVHHLLTQCAEATHDVMKPYGDLELTIACSSGVAVLWLTPRLPEFRAA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCHHHHC
YPNIKLRLIVRDGLASMSPAEFDVGVYYVRQQAPAEYTARRLFDEEVFPVCAPGYLAGRT
CCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCC
LDAADLANETLLRLEDGQRQWMSWSEWFDQNDIDRQKAQPQDITINSYPQIVQMTILGQG
CCHHHHCCHHEEEECCCHHHHCCHHHHCCCCCCCHHHCCCCCEEECCCCCEEEEEEECCC
VSLGWRYMIDACIEAGVLVRVTDASASHGGGYYVISPNDRAQNQAARLFSRWLFEQAERQ
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]