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The map label for this gene is proP [H]

Identifier: 189352302

GI number: 189352302

Start: 350476

End: 351792

Strand: Reverse

Name: proP [H]

Synonym: BMULJ_03521

Alternate gene names: 189352302

Gene position: 351792-350476 (Counterclockwise)

Preceding gene: 189352303

Following gene: 189352301

Centisome position: 14.22

GC content: 65.22

Gene sequence:

>1317_bases
ATGCAAGGAACGCTTTCCCCGCGCGCCGCGCCGTCCGTCGACGCGGCCGCGCAGCAGCGCCGCCGCGCCATCATCGCGAC
CGTGATCGGCAACGGTCTCGAATGGTTCGACTTCACCGTCTACAGCTTCTTCGCGGTGATCATCGCGAAGCTGTTCTTTC
CGACCGGCAATGAACTGACATCCGTGCTGCTGACCGTCGCGACATTCGGCGTCGGGTTCTTCATGCGGCCGGTCGGCGGC
ATCGTGCTGGGCGTCTACGCGGACAAGGTCGGGCGCAAGGCCGCGCTGTCGCTCACCATCCTGCTGATGGCGTTCGGTAC
CGCGCTGATCGGGCTCGCGCCGACCTACGAACAGGCCGGCATCGCCGCGCCGATGCTGATCGTCGTCGCGCGCCTGCTGC
AAGGGTTTTCCGCCGGCGGCGAAATGGGCGGCGCTACCGCGTTCCTCACCGAATACGCGCCGCCGGAAAAGCGCGCGTAT
TACTCGAGCTGGATCCAGTCGAGTATCGGCTTCGCCGTCTTGCTCGGCGCGGCGACCGGCACGTTCGTGACCACGTCGCT
CGATACGCAGGCGCTCCATAGCTGGGGCTGGCGGCTGCCGTTCCTGCTCGGGATCGTCGTCGGGCCGGTCGGGTATTTCA
TCCGCAGCCATCTCGACGAGACGCCCGCCTTCAGCGCGGTCGAGGCGCAGGCGAAGGAGAGTTCGCCGCTGAAGGCCGTG
CTGTCGACCTATCCGCGCGAGACGTTCGCGAGTTTTGCGATGGTCATCCTGTGGACCGTCTGTACGTATGTGCTGCTGTT
CTATATGCCGACCTATTCGGTGCGCACGCTGCATCTGCCGCAGTCGACAGGGTTTGCGGCGGGGATGGTCGGCGGCTCGA
TGATCATGTGCTGCTCGCCGCTTGTCGGCAGGCTCGCCGATCTGTGGGGGCGCCGCGTGTTTCTCTCCGGTGCGGCGCTC
GCCATCCTCGCGCTCGCGTGGCCGATGTTCGCGTGGATCAATCATGCACCGGGGTTCGTCTCGTTGATCGCGTTTCAGGC
CGTGTTCGGTTTGCTGATCGCCGCTTATACGGGGCCGATTCTTGCCGCGTTCGCCGAGCTGTTTCCGACCAAGGTGCTGT
CGACCGGCCTTTCCGTTGCGTACAACTTCGCGGTGACAATCTTCGGCGGCTTCGCGCCGTTTCTGATGACCTGGCTGATC
GCCCGCACGGGCAGCAACATGGCGCCCGCCCTCTACGTGATCGCCGCGGCGGCGGTCAGTTTCGTCGGGACGCGCTTCGT
GAAGGACACGAAGCGAACCGCTTTCGCCAATCGATAA

Upstream 100 bases:

>100_bases
TGCGTGCATGCGAATCTTTCGTTTCAGAAAAAAATCGCGCTGTCCTTAGCATGAGGTTCACGCGGGGCGCCCGTCCCGTC
TTCTGACTGAGGAAGAGACC

Downstream 100 bases:

>100_bases
GGATCTTTCTCCATGACCATTACCGTGCTTCAGGGCGGCAACGTGCTCGACCTCGAACGAGGCGTGTTGCTCGAACATCA
TCATGTCGTGATCGAAGGCG

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQGTLSPRAAPSVDAAAQQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNELTSVLLTVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAFGTALIGLAPTYEQAGIAAPMLIVVARLLQGFSAGGEMGGATAFLTEYAPPEKRAY
YSSWIQSSIGFAVLLGAATGTFVTTSLDTQALHSWGWRLPFLLGIVVGPVGYFIRSHLDETPAFSAVEAQAKESSPLKAV
LSTYPRETFASFAMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFAAGMVGGSMIMCCSPLVGRLADLWGRRVFLSGAAL
AILALAWPMFAWINHAPGFVSLIAFQAVFGLLIAAYTGPILAAFAELFPTKVLSTGLSVAYNFAVTIFGGFAPFLMTWLI
ARTGSNMAPALYVIAAAAVSFVGTRFVKDTKRTAFANR

Sequences:

>Translated_438_residues
MQGTLSPRAAPSVDAAAQQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNELTSVLLTVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAFGTALIGLAPTYEQAGIAAPMLIVVARLLQGFSAGGEMGGATAFLTEYAPPEKRAY
YSSWIQSSIGFAVLLGAATGTFVTTSLDTQALHSWGWRLPFLLGIVVGPVGYFIRSHLDETPAFSAVEAQAKESSPLKAV
LSTYPRETFASFAMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFAAGMVGGSMIMCCSPLVGRLADLWGRRVFLSGAAL
AILALAWPMFAWINHAPGFVSLIAFQAVFGLLIAAYTGPILAAFAELFPTKVLSTGLSVAYNFAVTIFGGFAPFLMTWLI
ARTGSNMAPALYVIAAAAVSFVGTRFVKDTKRTAFANR
>Mature_438_residues
MQGTLSPRAAPSVDAAAQQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNELTSVLLTVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAFGTALIGLAPTYEQAGIAAPMLIVVARLLQGFSAGGEMGGATAFLTEYAPPEKRAY
YSSWIQSSIGFAVLLGAATGTFVTTSLDTQALHSWGWRLPFLLGIVVGPVGYFIRSHLDETPAFSAVEAQAKESSPLKAV
LSTYPRETFASFAMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFAAGMVGGSMIMCCSPLVGRLADLWGRRVFLSGAAL
AILALAWPMFAWINHAPGFVSLIAFQAVFGLLIAAYTGPILAAFAELFPTKVLSTGLSVAYNFAVTIFGGFAPFLMTWLI
ARTGSNMAPALYVIAAAAVSFVGTRFVKDTKRTAFANR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=34.7926267281106, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1788292, Length=443, Percent_Identity=30.6997742663657, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=33.8785046728972, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789941, Length=449, Percent_Identity=34.075723830735, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI87082404, Length=410, Percent_Identity=24.390243902439, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.5486725663717, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46750; Mature: 46750

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]