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The map label for this gene is amiE [H]

Identifier: 189352212

GI number: 189352212

Start: 251617

End: 252741

Strand: Reverse

Name: amiE [H]

Synonym: BMULJ_03430

Alternate gene names: 189352212

Gene position: 252741-251617 (Counterclockwise)

Preceding gene: 189352213

Following gene: 189352211

Centisome position: 10.22

GC content: 73.24

Gene sequence:

>1125_bases
ATGACAGGCTTCATCGACACCTTCACGCTCGGCGGCCCGGGGCCGACGATCGCCATCAAGGACACGATCGACATCGCGGG
CTATCCGACGCGCGCGGCGAGCCGCGCGCTCGCCGACGCACCGCCCGCCACGCAACACGCCGACGTCGTGCGTCTGCTGC
TCGACGCCGGCTGGCAGATCGCCGGCAAGGCCAACATGCACGAGCTCGCGTTCGGCATGACGGGCATCAACGACTACACC
GGCACGCCGCTCAATCCGCAGGATGCGTCGCGCATTCCCGGCGGTTCGTCGAGCGGCTCCGCGTCGCTTGTCGGGCTCGG
CGCCGTCGATGCGGCGCTCGGCACCGACACCGGCGGCTCGATTCGCGGGCCGGCCGCATGCTGCGGCGTGTTCGGGCTGA
AACCGACGTTCGGCCGCGTGTCGCGGCGCGGCGTCGCGCCTGCCGAAACGACGCTCGACTGTGTCGGGCCGTTCGCGCGC
GACATGGATACACTGAGCGCCGCGATGGCCGCGATCGCGCCGGGCTTCGACCGCGTGCGCGCGGCGGCGTCGGCCGCCGG
CTGCACGGTCGCGCAGGTCGGCGTCGATGCGGAGCCGGCCATCGCCCGCGCATTGCGTGCGGCGCTCGATGCGGCCGGCC
TGCGCACGCACACGCTCGTGCTCGACGATCTGCATGCGGCATTCGATGCGGGGCTCGTCGTCATCAACGCCGAAACGTCG
CGCGCATTCGGCCATCTGGTCGCGACCGGCCGGCTCGGCGCGGATCTCGATGCGCGGCTGCGCGCGGCGGCGACGACGAC
GGCCGCCGCGCTCGACGCGGCCGAGGCCGTGCGCGCGCGTTTCACCGCGCAGGTCGACGCGGCGCTCGAACACGCGGACG
CACTCGTGCTGCCGACGCTGCCCGCGCTGCCGATCACGCTCGATGAGGCGCGCAACGGCGTGTCGGTGATCGCGATGTCG
TCGCTGATCCGGCCGTTCAACCTGAGCGGCCATCCGGCGCTCAGCGTGCCGCTGCCGCTCGACGGCTCGCCGCTGAAGGC
CGGGCTGCAGATCGTCGGACGCAAGGGCGCGGACGAACAGGTGTGCGCGCTCGCCGCGCATGTCGCCGCGGCGCTCGCCG
CCTGA

Upstream 100 bases:

>100_bases
ATCCGGCATTTCCGCACCGAGCGCGTGCTCGACGGCCCGTGGCCGCTCGCTGCCGCGCAACGGCCCTGAACTTCTCCACC
GCGCTCGCGCGACATCGCAT

Downstream 100 bases:

>100_bases
ACCACGATGCGTGCGCGCATGCGCATGCGCATGCGAACCACGGAATCACGCACCATGTCAGACCGAGTCGTACTCGTCAC
CGGCGCCGCCCGCGGGCTCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTGFIDTFTLGGPGPTIAIKDTIDIAGYPTRAASRALADAPPATQHADVVRLLLDAGWQIAGKANMHELAFGMTGINDYT
GTPLNPQDASRIPGGSSSGSASLVGLGAVDAALGTDTGGSIRGPAACCGVFGLKPTFGRVSRRGVAPAETTLDCVGPFAR
DMDTLSAAMAAIAPGFDRVRAAASAAGCTVAQVGVDAEPAIARALRAALDAAGLRTHTLVLDDLHAAFDAGLVVINAETS
RAFGHLVATGRLGADLDARLRAAATTTAAALDAAEAVRARFTAQVDAALEHADALVLPTLPALPITLDEARNGVSVIAMS
SLIRPFNLSGHPALSVPLPLDGSPLKAGLQIVGRKGADEQVCALAAHVAAALAA

Sequences:

>Translated_374_residues
MTGFIDTFTLGGPGPTIAIKDTIDIAGYPTRAASRALADAPPATQHADVVRLLLDAGWQIAGKANMHELAFGMTGINDYT
GTPLNPQDASRIPGGSSSGSASLVGLGAVDAALGTDTGGSIRGPAACCGVFGLKPTFGRVSRRGVAPAETTLDCVGPFAR
DMDTLSAAMAAIAPGFDRVRAAASAAGCTVAQVGVDAEPAIARALRAALDAAGLRTHTLVLDDLHAAFDAGLVVINAETS
RAFGHLVATGRLGADLDARLRAAATTTAAALDAAEAVRARFTAQVDAALEHADALVLPTLPALPITLDEARNGVSVIAMS
SLIRPFNLSGHPALSVPLPLDGSPLKAGLQIVGRKGADEQVCALAAHVAAALAA
>Mature_373_residues
TGFIDTFTLGGPGPTIAIKDTIDIAGYPTRAASRALADAPPATQHADVVRLLLDAGWQIAGKANMHELAFGMTGINDYTG
TPLNPQDASRIPGGSSSGSASLVGLGAVDAALGTDTGGSIRGPAACCGVFGLKPTFGRVSRRGVAPAETTLDCVGPFARD
MDTLSAAMAAIAPGFDRVRAAASAAGCTVAQVGVDAEPAIARALRAALDAAGLRTHTLVLDDLHAAFDAGLVVINAETSR
AFGHLVATGRLGADLDARLRAAATTTAAALDAAEAVRARFTAQVDAALEHADALVLPTLPALPITLDEARNGVSVIAMSS
LIRPFNLSGHPALSVPLPLDGSPLKAGLQIVGRKGADEQVCALAAHVAAALAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=180, Percent_Identity=34.4444444444444, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI195972892, Length=172, Percent_Identity=33.1395348837209, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=100, Percent_Identity=43, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=363, Percent_Identity=25.8953168044077, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=157, Percent_Identity=31.2101910828025, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=423, Percent_Identity=26.0047281323877, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=149, Percent_Identity=45.6375838926174, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648113, Length=167, Percent_Identity=44.311377245509, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644968, Length=407, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21356731, Length=134, Percent_Identity=37.3134328358209, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550774, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648435, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648437, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648441, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648439, Length=134, Percent_Identity=37.3134328358209, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 37537; Mature: 37406

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFIDTFTLGGPGPTIAIKDTIDIAGYPTRAASRALADAPPATQHADVVRLLLDAGWQI
CCCCCCEEECCCCCCEEEEECCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
AGKANMHELAFGMTGINDYTGTPLNPQDASRIPGGSSSGSASLVGLGAVDAALGTDTGGS
ECCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHCCCCCCC
IRGPAACCGVFGLKPTFGRVSRRGVAPAETTLDCVGPFARDMDTLSAAMAAIAPGFDRVR
CCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
AAASAAGCTVAQVGVDAEPAIARALRAALDAAGLRTHTLVLDDLHAAFDAGLVVINAETS
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCEEEEECCCC
RAFGHLVATGRLGADLDARLRAAATTTAAALDAAEAVRARFTAQVDAALEHADALVLPTL
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PALPITLDEARNGVSVIAMSSLIRPFNLSGHPALSVPLPLDGSPLKAGLQIVGRKGADEQ
CCCCEEHHHCCCCEEHEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHH
VCALAAHVAAALAA
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TGFIDTFTLGGPGPTIAIKDTIDIAGYPTRAASRALADAPPATQHADVVRLLLDAGWQI
CCCCCEEECCCCCCEEEEECCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
AGKANMHELAFGMTGINDYTGTPLNPQDASRIPGGSSSGSASLVGLGAVDAALGTDTGGS
ECCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHCCCCCCC
IRGPAACCGVFGLKPTFGRVSRRGVAPAETTLDCVGPFARDMDTLSAAMAAIAPGFDRVR
CCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
AAASAAGCTVAQVGVDAEPAIARALRAALDAAGLRTHTLVLDDLHAAFDAGLVVINAETS
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCEEEEECCCC
RAFGHLVATGRLGADLDARLRAAATTTAAALDAAEAVRARFTAQVDAALEHADALVLPTL
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PALPITLDEARNGVSVIAMSSLIRPFNLSGHPALSVPLPLDGSPLKAGLQIVGRKGADEQ
CCCCEEHHHCCCCEEHEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHH
VCALAAHVAAALAA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]