BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome 2, complete genome.
Accession	NC_010805
Length	2,473,162

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The map label for this gene is polS [H]

Identifier: 189352211

GI number: 189352211

Start: 250819

End: 251562

Strand: Reverse

Name: polS [H]

Synonym: BMULJ_03429

Alternate gene names: 189352211

Gene position: 251562-250819 (Counterclockwise)

Preceding gene: 189352212

Following gene: 189352209

Centisome position: 10.17

GC content: 69.35

Gene sequence:

>744_bases
ATGTCAGACCGAGTCGTACTCGTCACCGGCGCCGCCCGCGGGCTCGGCGCCGTCATTGCCGAACGCTTTCATGCGGCCGG
CTATCGCGTCGCGCTGGCCGACATCGCGGCCGACGCGATCCGCGCACACGCACGCGATCTCGATCCGGAAGGCACGCGCG
CGATCGCGATACCGCTCGACGTCACGTCGAAACACGACTTCGAAGCGGCGCGCGATGCGCTCGTCGCGCGCTGGGGCGAG
GTCGACGTGCTCGTCAACAATGCGGGCGCATCGAAGGTCGTGCCCGCGATGGAGATCACCGCCGAACAGTTCGATCAGGT
GATCGACGTGAACCTGCGCAGCGTGCTGTTCGGGTGTCAGGTATTCGGCCAGTATTTCGCGACGCGCGGTGCCGGCCGCA
TCGTCAACATCGCGTCGCTTGCCGGCCAGAACGGCGGCTCCGCGACGGGCGCGCACTACGCCGCCGCGAAAGGCGGCACG
CTGACGCTGACCAAGGTGTTCGCGCGCGACCTCGGCGCGCACGGCGTGACCGTGAATGCGATCTCGCCCGGCCCGCTGGA
CCTGCCGATCGTCCATGAAAGCGTCGCGCCCGAAAAGCTGCGTCAGGTGCTCGCGAGCCTGCCGGGCGGCCGGCTCGGCT
CGGCCGGCTTCGTCGCCGACGCGGCCGTGCTGCTCGCGTCGGGCGACGCGTATTTCGCGAACGGCGCGTGCTGGGACATC
AACGGCGGCTTGTACATGCGCTGA

Upstream 100 bases:

>100_bases
GGTGTGCGCGCTCGCCGCGCATGTCGCCGCGGCGCTCGCCGCCTGAACCACGATGCGTGCGCGCATGCGCATGCGCATGC
GAACCACGGAATCACGCACC

Downstream 100 bases:

>100_bases
GCGCGCGAGGTACGCAGCCCGCGCCGCGGGCCGCAACCGTTTCGTCGGCCGCTATCGCGGCCGCTCGCGTTCGTCGCCCC
AGACGACCATCACGTCGCGC

Product: putative short-chain alcohol dehydrogenase

Products: NA

Alternate protein names: L-iditol 2-dehydrogenase; Polyol dehydrogenase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSDRVVLVTGAARGLGAVIAERFHAAGYRVALADIAADAIRAHARDLDPEGTRAIAIPLDVTSKHDFEAARDALVARWGE
VDVLVNNAGASKVVPAMEITAEQFDQVIDVNLRSVLFGCQVFGQYFATRGAGRIVNIASLAGQNGGSATGAHYAAAKGGT
LTLTKVFARDLGAHGVTVNAISPGPLDLPIVHESVAPEKLRQVLASLPGGRLGSAGFVADAAVLLASGDAYFANGACWDI
NGGLYMR

Sequences:

>Translated_247_residues
MSDRVVLVTGAARGLGAVIAERFHAAGYRVALADIAADAIRAHARDLDPEGTRAIAIPLDVTSKHDFEAARDALVARWGE
VDVLVNNAGASKVVPAMEITAEQFDQVIDVNLRSVLFGCQVFGQYFATRGAGRIVNIASLAGQNGGSATGAHYAAAKGGT
LTLTKVFARDLGAHGVTVNAISPGPLDLPIVHESVAPEKLRQVLASLPGGRLGSAGFVADAAVLLASGDAYFANGACWDI
NGGLYMR
>Mature_246_residues
SDRVVLVTGAARGLGAVIAERFHAAGYRVALADIAADAIRAHARDLDPEGTRAIAIPLDVTSKHDFEAARDALVARWGEV
DVLVNNAGASKVVPAMEITAEQFDQVIDVNLRSVLFGCQVFGQYFATRGAGRIVNIASLAGQNGGSATGAHYAAAKGGTL
TLTKVFARDLGAHGVTVNAISPGPLDLPIVHESVAPEKLRQVLASLPGGRLGSAGFVADAAVLLASGDAYFANGACWDIN
GGLYMR

Specific function: Catalyzes the oxidation of D-glucitol (sorbitol) to D- fructose, galactitol to D-tagatose and of L-iditol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=247, Percent_Identity=31.9838056680162, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=27.7551020408163, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI59889578, Length=227, Percent_Identity=33.0396475770925, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI4758504, Length=258, Percent_Identity=33.7209302325581, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=28.4046692607004, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI19923817, Length=256, Percent_Identity=24.609375, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI31542939, Length=203, Percent_Identity=31.5270935960591, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI83715985, Length=258, Percent_Identity=32.1705426356589, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI66933014, Length=190, Percent_Identity=27.8947368421053, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI7706318, Length=208, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=25.7028112449799, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4503301, Length=222, Percent_Identity=24.7747747747748, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI150247226, Length=196, Percent_Identity=30.6122448979592, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5031737, Length=238, Percent_Identity=27.7310924369748, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI50726952, Length=216, Percent_Identity=26.8518518518519, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=35.0806451612903, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=31.5789473684211, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=29.718875502008, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787905, Length=240, Percent_Identity=30.8333333333333, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=28.4, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082100, Length=251, Percent_Identity=30.6772908366534, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788459, Length=248, Percent_Identity=26.6129032258064, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789057, Length=258, Percent_Identity=29.0697674418605, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=28.6885245901639, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI2367365, Length=241, Percent_Identity=31.1203319502075, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787820, Length=185, Percent_Identity=32.972972972973, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=31.1740890688259, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=30.6122448979592, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=28.90625, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=218, Percent_Identity=33.9449541284404, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=26.6129032258064, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=190, Percent_Identity=27.3684210526316, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=248, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=173, Percent_Identity=30.0578034682081, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=190, Percent_Identity=26.8421052631579, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=190, Percent_Identity=27.8947368421053, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=183, Percent_Identity=28.9617486338798, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17536651, Length=246, Percent_Identity=24.7967479674797, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=192, Percent_Identity=28.125, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=195, Percent_Identity=27.1794871794872, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=243, Percent_Identity=26.3374485596708, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=194, Percent_Identity=28.8659793814433, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=243, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=243, Percent_Identity=27.1604938271605, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=247, Percent_Identity=24.6963562753036, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=233, Percent_Identity=27.0386266094421, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=253, Percent_Identity=25.6916996047431, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=252, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=194, Percent_Identity=26.2886597938144, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=243, Percent_Identity=25.5144032921811, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=250, Percent_Identity=24.8, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=192, Percent_Identity=23.9583333333333, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=194, Percent_Identity=23.7113402061856, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=154, Percent_Identity=27.9220779220779, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=188, Percent_Identity=31.3829787234043, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324126, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=34.1269841269841, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=29.4354838709677, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17737361, Length=260, Percent_Identity=31.5384615384615, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=28.2868525896414, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=27.8884462151394, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=27.8884462151394, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=24.4979919678715, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=29.8804780876494, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357673, Length=188, Percent_Identity=25, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 25517; Mature: 25386

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVVLVTGAARGLGAVIAERFHAAGYRVALADIAADAIRAHARDLDPEGTRAIAIPLD
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
VTSKHDFEAARDALVARWGEVDVLVNNAGASKVVPAMEITAEQFDQVIDVNLRSVLFGCQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VFGQYFATRGAGRIVNIASLAGQNGGSATGAHYAAAKGGTLTLTKVFARDLGAHGVTVNA
HHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCCCEEEEE
ISPGPLDLPIVHESVAPEKLRQVLASLPGGRLGSAGFVADAAVLLASGDAYFANGACWDI
CCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHEEEEEECCCEEEECCEEEEC
NGGLYMR
CCCEEEC
>Mature Secondary Structure 
SDRVVLVTGAARGLGAVIAERFHAAGYRVALADIAADAIRAHARDLDPEGTRAIAIPLD
CCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
VTSKHDFEAARDALVARWGEVDVLVNNAGASKVVPAMEITAEQFDQVIDVNLRSVLFGCQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VFGQYFATRGAGRIVNIASLAGQNGGSATGAHYAAAKGGTLTLTKVFARDLGAHGVTVNA
HHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCCCEEEEE
ISPGPLDLPIVHESVAPEKLRQVLASLPGGRLGSAGFVADAAVLLASGDAYFANGACWDI
CCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHEEEEEECCCEEEECCEEEEC
NGGLYMR
CCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7551049 [H]