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The map label for this gene is rhtC [H]

Identifier: 189352157

GI number: 189352157

Start: 185520

End: 186134

Strand: Reverse

Name: rhtC [H]

Synonym: BMULJ_03375

Alternate gene names: 189352157

Gene position: 186134-185520 (Counterclockwise)

Preceding gene: 189352158

Following gene: 189352153

Centisome position: 7.53

GC content: 70.57

Gene sequence:

>615_bases
ATGCCTGCCCATCTCCATCTGTTGCTGCCGCTCGCCGGCGTGCTGCTGTTGAGCGTCGTCAGCCCCGGACCGAACTTCGT
GATCGTCACGTCGACGGCGGTCGCGTCGCGCCGCGCGGGCGTGATGACGAGCGTCGGCCTCGCGGCCGCGTCCGGCACGT
GGGCGGCAATCGCGATCGCGGGGCTGAGCCTGCTCGTCACGCATGTCGCGTGGGTGCATACCGCGCTGCGGATCGCGGGC
GCAACTTATCTGCTGTGGCTCGGCGCGAAGATGATCGCGACCGCGCGACGGCCGCTCGCCCTGTCGACCGAGCCCGGCCG
CTCGGGATGGCGCGCCGCGCGCAACGGCTACGTGGTCAGCATGACGAATCCGAAGGCCGTCGCGTTCTACGGGAGCGTGT
TTGCGCTGATGGTGCCCGCGCACGCGCCGGCGTGGTTCGCACCGGCCGTCGTCGGCATCTCGATTGCAATTTCGGGCGCA
TGGTACGGCGCGATGGCGCTGCTCGCGTCGCATCCGACGGTGCGCGGGCTGCTGATCCGGCGCAAGGCCGTGCTCGATAC
GACGGCCGGCGTGCTGCTGATGGGACTCGGCGGGAAGATGCTGGCGGGGCGATGA

Upstream 100 bases:

>100_bases
AAGGAAGACCTCGCGCTGTTCAAGACCGTGTCGCTGCTGATCTGACGCGCCGCCGAGCGGTTAAGATGGAAGCCTTTCCC
GCGTCTGCCGAGGAGTTCCG

Downstream 100 bases:

>100_bases
TGTACGGCGCGGTGCAGCGCGCGCGTTCCGCGACGGTGCGCGCCGCGCGCTGCGGCGGCAGCTCGCGAGCGACTCGCTCG
CGGCGCGCCGTCGCAACGCA

Product: threonine efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPAHLHLLLPLAGVLLLSVVSPGPNFVIVTSTAVASRRAGVMTSVGLAAASGTWAAIAIAGLSLLVTHVAWVHTALRIAG
ATYLLWLGAKMIATARRPLALSTEPGRSGWRAARNGYVVSMTNPKAVAFYGSVFALMVPAHAPAWFAPAVVGISIAISGA
WYGAMALLASHPTVRGLLIRRKAVLDTTAGVLLMGLGGKMLAGR

Sequences:

>Translated_204_residues
MPAHLHLLLPLAGVLLLSVVSPGPNFVIVTSTAVASRRAGVMTSVGLAAASGTWAAIAIAGLSLLVTHVAWVHTALRIAG
ATYLLWLGAKMIATARRPLALSTEPGRSGWRAARNGYVVSMTNPKAVAFYGSVFALMVPAHAPAWFAPAVVGISIAISGA
WYGAMALLASHPTVRGLLIRRKAVLDTTAGVLLMGLGGKMLAGR
>Mature_203_residues
PAHLHLLLPLAGVLLLSVVSPGPNFVIVTSTAVASRRAGVMTSVGLAAASGTWAAIAIAGLSLLVTHVAWVHTALRIAGA
TYLLWLGAKMIATARRPLALSTEPGRSGWRAARNGYVVSMTNPKAVAFYGSVFALMVPAHAPAWFAPAVVGISIAISGAW
YGAMALLASHPTVRGLLIRRKAVLDTTAGVLLMGLGGKMLAGR

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=206, Percent_Identity=33.0097087378641, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1786522, Length=144, Percent_Identity=29.8611111111111, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788099, Length=129, Percent_Identity=30.2325581395349, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 20992; Mature: 20861

Theoretical pI: Translated: 12.37; Mature: 12.37

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHLHLLLPLAGVLLLSVVSPGPNFVIVTSTAVASRRAGVMTSVGLAAASGTWAAIAIA
CCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCHHHHHHHHCCCCHHHHHHH
GLSLLVTHVAWVHTALRIAGATYLLWLGAKMIATARRPLALSTEPGRSGWRAARNGYVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCEEEE
MTNPKAVAFYGSVFALMVPAHAPAWFAPAVVGISIAISGAWYGAMALLASHPTVRGLLIR
ECCCCEEEHHHHHHHHEECCCCCCHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHH
RKAVLDTTAGVLLMGLGGKMLAGR
HHHHHHHHHHEEEEECCCHHCCCC
>Mature Secondary Structure 
PAHLHLLLPLAGVLLLSVVSPGPNFVIVTSTAVASRRAGVMTSVGLAAASGTWAAIAIA
CCEEHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCHHHHHHHHCCCCHHHHHHH
GLSLLVTHVAWVHTALRIAGATYLLWLGAKMIATARRPLALSTEPGRSGWRAARNGYVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCEEEE
MTNPKAVAFYGSVFALMVPAHAPAWFAPAVVGISIAISGAWYGAMALLASHPTVRGLLIR
ECCCCEEEHHHHHHHHEECCCCCCHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHH
RKAVLDTTAGVLLMGLGGKMLAGR
HHHHHHHHHHEEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]