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The map label for this gene is traA [H]

Identifier: 189184802

GI number: 189184802

Start: 1933705

End: 1934796

Strand: Direct

Name: traA [H]

Synonym: OTT_1895

Alternate gene names: 189184802

Gene position: 1933705-1934796 (Clockwise)

Preceding gene: 189184801

Following gene: 189184803

Centisome position: 96.25

GC content: 34.16

Gene sequence:

>1092_bases
TTGAAGATTATTAATGATGCTGACGTAATAACGGATTCTATAACACATTACAAATCTATTTTTACTAAGCAGGATGTTGA
AAAAGCAGTAAAAGATATACCAGATCTAACAGCAAGAGAACAGTTAGTTCAGCAAGTGCTTAGTTCAAATAGAATACTAG
AGTTATACCATGATGATGGTGAAAGTAGCAAATATTTTACGACAATTGAGGTTCGAAATGAGGAGACGAGAATAATCAGA
ATAGCTAATAAAATCAATGATCAGGTTTATTACAACAACATTTACAATCTTAAAAGTGATATCGAAGGTCTAGCAAATGT
TAGTGAGGAACAGAAACAAGCGCTAAGGCATATTTTGCTTAGCACTAGTGGAGTTAGAGTCTTGAGAGGAAGAGCTGGCA
CAGGAAAATCTTATGTTTTAATAAAAGCGCATAAGCTCGCAACAAATCGTGGACAAAAAGTTATTGGTCTTGCTCCTACT
CATAAGGCAGTATCAGAGCTGAGGAGCAAAGGTTATACAGAGGTCTATACAGTAAAAGGATTTTTATATAATCGAAAAAA
AATTTTTATGCAAGACAGCTTAATAGTAGTAGATGAAGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGCTGTTTAGAG
TAGTTAGAAACAATAATTGTCAACTGATACTTGCTGGAGATGAAAAGCAGTTAGCTTCAATAGAAAGAGGTGGAATGTTT
GAGATGCTGAGTAATATTTTTGGTTCACATGTTTTAGTAAATATTCGAAGACAAAGTAAAAACTGGAGCAGAAAAGCAGC
AATGGAGTTTGCTGAGAGTAATATTTTAAGCGGTATAACCTTACTGAGGCAAAATAACTGCGTTAGGTTTGATAATACGT
TGCAGGACTCAATGAGTAAGTTAATATACAACTGGAGTTTAAGCAAATTTAAACCACATGAAAAACTGGTAATTACAGTA
CGTAATAAAGATGTCGACATTCTTAATTCAAGTATTAGGTCTTTGTTAAAAGCAAATGGTACGCTACAAGGCAAAGAATA
TAGGTGTTCATCAATAGCTGGAAAAAAAAGAGTCCTATATGGTAGGAGATAG

Upstream 100 bases:

>100_bases
AGGTAGAACGAACCGCAAAAAACCAAAAAAACAGCCAGTTGTTGAAGGATATCGTAATAATGAAGTATTAAATGAAAATG
AGTTACGTAAAGAGGCTCAT

Downstream 100 bases:

>100_bases
AATCGTATTTCAAAAAAGCAATAAGGATTTACAAATACAAAACAGTGAATTTGCAACTTTAACTTCTGTTAATAAAAATG
AATTTGTAGCTAAGACAGAT

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIR
IANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPT
HKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
EMLSNIFGSHVLVNIRRQSKNWSRKAAMEFAESNILSGITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITV
RNKDVDILNSSIRSLLKANGTLQGKEYRCSSIAGKKRVLYGRR

Sequences:

>Translated_363_residues
MKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIR
IANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPT
HKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
EMLSNIFGSHVLVNIRRQSKNWSRKAAMEFAESNILSGITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITV
RNKDVDILNSSIRSLLKANGTLQGKEYRCSSIAGKKRVLYGRR
>Mature_363_residues
MKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIR
IANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPT
HKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
EMLSNIFGSHVLVNIRRQSKNWSRKAAMEFAESNILSGITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITV
RNKDVDILNSSIRSLLKANGTLQGKEYRCSSIAGKKRVLYGRR

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 41258; Mature: 41258

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCC
ESSKYFTTIEVRNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILL
CCCEEEEEEEEECCCCEEEEEEHHHCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHH
STSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKG
CCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEE
FLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
EEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHH
EMLSNIFGSHVLVNIRRQSKNWSRKAAMEFAESNILSGITLLRQNNCVRFDNTLQDSMSK
HHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHH
LIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLQGKEYRCSSIAGKKRVLY
HHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCC
GRR
CCC
>Mature Secondary Structure
MKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCC
ESSKYFTTIEVRNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILL
CCCEEEEEEEEECCCCEEEEEEHHHCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHH
STSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKG
CCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEE
FLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
EEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHH
EMLSNIFGSHVLVNIRRQSKNWSRKAAMEFAESNILSGITLLRQNNCVRFDNTLQDSMSK
HHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHH
LIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLQGKEYRCSSIAGKKRVLY
HHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCC
GRR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]