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The map label for this gene is tdcB [H]

Identifier: 189184536

GI number: 189184536

Start: 1657150

End: 1658163

Strand: Direct

Name: tdcB [H]

Synonym: OTT_1629

Alternate gene names: 189184536

Gene position: 1657150-1658163 (Clockwise)

Preceding gene: 189184534

Following gene: 189184539

Centisome position: 82.49

GC content: 31.76

Gene sequence:

>1014_bases
ATGTTAAGTATTATTAAAGCTTATAATCGAATTAAGCCATATATCTATCAAACCCCTATTCTATATTCTGATTACCTAAA
CCACGTTTTAGGACATGAAATATACTTTAAAGCAGAATCATTACAGAAATCTGGTTCATTTAAGATAAGAGGAGTACTTA
ATCATTTACTTGCATTGCAAGAAAAGAAATTACTACCAACAAAAATTTCTACTTATACTACTGGAAATCATGGAGTGGCA
GTAGCATTGGTAGCTAAATCACTAAAATTAAATACTGTTAAAATATACTCTAACTGCATTTCTAAAACAAAATTACATAT
GGCAAACCATTATGGAGCAAAGGTAATTTTGACAAATTCAAGAATTGAAGCAGAGCAAAAAGCAAAGTTAGAGCAAGACA
ATGAATTTTATTATCTACACCCATCAGATAGCGATAGTATTATTGCAGGAGCTGGAACTGTATATTATGAAGCTCTGCAA
CAATTACACTTTTCTCCTGATGCAGTATTTGCTTCTTGTGGTGGAGGAGGATTATTATCAGGCACATACCTAACTAAAAT
TAGAACTGATACTTTAGTTCAAATTATAGGATCTGAACCAGTAAATGCTAACGATGCATTTACTTCGTTGTGTCATGGCA
ATATTTTTACCTTGCCTAAATCACCAAATACAGTTGCTGATGGTCTTAGTGCATTAAACCTATCAGAAAGAACTTTTCAA
TATATCAAAAAACTAGATGATATGGTACTTGTTCCGGAAAAATCAATTTATTATTGGACTACTTGGCTTATTACTTTACT
CAAAATTACTTGTGAACCATCAAGCGCAATTAATATGGCTGCAGTGTGTAATTGGCTTTTAAGGCAAAATTCACCCAAAA
AGGCTTTAGTTATACTATCTGGTGGTAACATTGACCCAAATACACACTCAAAACTAATGCAGGATAAATTTATACAACAA
ATTCCTAAAATTGAAGAATGTAGCAAGGGAACTATTTCATTCAAAAATTCTTGA

Upstream 100 bases:

>100_bases
TTTATTTACTAGTATATCATGATAATCTATATAATCACACAGATAATTAAAATCAATTATTTTACGTATGAATATTTTGC
ATAGTAGATAGTATTTTTGT

Downstream 100 bases:

>100_bases
TGATATAGCCTTGTATATTGAAAATATGTGGAATTGCAATAAAATAAAAGTTATATTAATAGCATCAAAGAAATCTAGTA
TACAGTTTTGATCCTGAGGC

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLSIIKAYNRIKPYIYQTPILYSDYLNHVLGHEIYFKAESLQKSGSFKIRGVLNHLLALQEKKLLPTKISTYTTGNHGVA
VALVAKSLKLNTVKIYSNCISKTKLHMANHYGAKVILTNSRIEAEQKAKLEQDNEFYYLHPSDSDSIIAGAGTVYYEALQ
QLHFSPDAVFASCGGGGLLSGTYLTKIRTDTLVQIIGSEPVNANDAFTSLCHGNIFTLPKSPNTVADGLSALNLSERTFQ
YIKKLDDMVLVPEKSIYYWTTWLITLLKITCEPSSAINMAAVCNWLLRQNSPKKALVILSGGNIDPNTHSKLMQDKFIQQ
IPKIEECSKGTISFKNS

Sequences:

>Translated_337_residues
MLSIIKAYNRIKPYIYQTPILYSDYLNHVLGHEIYFKAESLQKSGSFKIRGVLNHLLALQEKKLLPTKISTYTTGNHGVA
VALVAKSLKLNTVKIYSNCISKTKLHMANHYGAKVILTNSRIEAEQKAKLEQDNEFYYLHPSDSDSIIAGAGTVYYEALQ
QLHFSPDAVFASCGGGGLLSGTYLTKIRTDTLVQIIGSEPVNANDAFTSLCHGNIFTLPKSPNTVADGLSALNLSERTFQ
YIKKLDDMVLVPEKSIYYWTTWLITLLKITCEPSSAINMAAVCNWLLRQNSPKKALVILSGGNIDPNTHSKLMQDKFIQQ
IPKIEECSKGTISFKNS
>Mature_337_residues
MLSIIKAYNRIKPYIYQTPILYSDYLNHVLGHEIYFKAESLQKSGSFKIRGVLNHLLALQEKKLLPTKISTYTTGNHGVA
VALVAKSLKLNTVKIYSNCISKTKLHMANHYGAKVILTNSRIEAEQKAKLEQDNEFYYLHPSDSDSIIAGAGTVYYEALQ
QLHFSPDAVFASCGGGGLLSGTYLTKIRTDTLVQIIGSEPVNANDAFTSLCHGNIFTLPKSPNTVADGLSALNLSERTFQ
YIKKLDDMVLVPEKSIYYWTTWLITLLKITCEPSSAINMAAVCNWLLRQNSPKKALVILSGGNIDPNTHSKLMQDKFIQQ
IPKIEECSKGTISFKNS

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=307, Percent_Identity=31.9218241042345, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1789505, Length=315, Percent_Identity=29.8412698412698, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790207, Length=293, Percent_Identity=28.3276450511945, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=27.6190476190476, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17537387, Length=313, Percent_Identity=30.9904153354633, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71991565, Length=306, Percent_Identity=27.1241830065359, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322631, Length=319, Percent_Identity=33.8557993730408, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320930, Length=301, Percent_Identity=29.5681063122924, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319788, Length=257, Percent_Identity=26.8482490272374, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355833, Length=309, Percent_Identity=30.0970873786408, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 37460; Mature: 37460

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIIKAYNRIKPYIYQTPILYSDYLNHVLGHEIYFKAESLQKSGSFKIRGVLNHLLALQ
CCHHHHHHHHCCCEEEECCCHHHHHHHHHHCCEEEEEHHHHCCCCCEEHHHHHHHHHHHH
EKKLLPTKISTYTTGNHGVAVALVAKSLKLNTVKIYSNCISKTKLHMANHYGAKVILTNS
HCCCCCCEEEEEECCCCCEEEEEEEHHHCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECC
RIEAEQKAKLEQDNEFYYLHPSDSDSIIAGAGTVYYEALQQLHFSPDAVFASCGGGGLLS
CCCHHHHHCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
GTYLTKIRTDTLVQIIGSEPVNANDAFTSLCHGNIFTLPKSPNTVADGLSALNLSERTFQ
CEEEEEECHHHHHHHHCCCCCCCCHHHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHHH
YIKKLDDMVLVPEKSIYYWTTWLITLLKITCEPSSAINMAAVCNWLLRQNSPKKALVILS
HHHHHCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
GGNIDPNTHSKLMQDKFIQQIPKIEECSKGTISFKNS
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEECCC
>Mature Secondary Structure
MLSIIKAYNRIKPYIYQTPILYSDYLNHVLGHEIYFKAESLQKSGSFKIRGVLNHLLALQ
CCHHHHHHHHCCCEEEECCCHHHHHHHHHHCCEEEEEHHHHCCCCCEEHHHHHHHHHHHH
EKKLLPTKISTYTTGNHGVAVALVAKSLKLNTVKIYSNCISKTKLHMANHYGAKVILTNS
HCCCCCCEEEEEECCCCCEEEEEEEHHHCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECC
RIEAEQKAKLEQDNEFYYLHPSDSDSIIAGAGTVYYEALQQLHFSPDAVFASCGGGGLLS
CCCHHHHHCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
GTYLTKIRTDTLVQIIGSEPVNANDAFTSLCHGNIFTLPKSPNTVADGLSALNLSERTFQ
CEEEEEECHHHHHHHHCCCCCCCCHHHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHHH
YIKKLDDMVLVPEKSIYYWTTWLITLLKITCEPSSAINMAAVCNWLLRQNSPKKALVILS
HHHHHCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
GGNIDPNTHSKLMQDKFIQQIPKIEECSKGTISFKNS
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]