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The map label for this gene is miaA [H]

Identifier: 189184532

GI number: 189184532

Start: 1652050

End: 1653087

Strand: Direct

Name: miaA [H]

Synonym: OTT_1625

Alternate gene names: 189184532

Gene position: 1652050-1653087 (Clockwise)

Preceding gene: 189184526

Following gene: 189184533

Centisome position: 82.23

GC content: 28.9

Gene sequence:

>1038_bases
ATGAATGATATATTTATAATTGCTGGGCCTACAGCTAGTGGTAAATCTGAACTAGCAATGTTATTAGCGCAAAAATTCAA
TGGTGTTATTATTAATGCTGACTCAATGCAAATATATAAAGAAATTCCAATAATTACAGCTTCTCCAAGCATTTCAGAAA
AACAGCAGATTGATCACTATTTATATAATTATGTATCAATTTTTCATTCTGATATTAATGAACTGAATTCCTTCGATAAT
ATTTCTCAATCTAAAGTAAATGCTGATTATAATAATATAAACTTACAGCAATCTATAACAAAACACTTAACAGATAGGAG
GTATTCGGTAGCCAAGTATGTACAAGAAGCTTGTAAAATTATTAGAAGTGTTATACATGCATTAAAATTACCAATTGTTG
TTGGAGGTAGTGGAATGTATATTAATGCGCTAGTGTATGGTATTCATCATATTCCTGAAATAACTTTGGAGATTCGTATG
CAAGTTCAAGATCTCTATAAAAAACTAACTAAACAAGAATTTTATCAAAAATTGATAGATTTAGACCCTATTTCTAAGAA
TTACATTCATTCAAGTGATGCTCAGAGAATGATTAGGGCATATGAAGTTGTGCTGCAAACTAATAAATCAATTTTTAGTT
ACCATAACTCTAAACTAGTTTCTCCTCTTGAAGGTTACAATGTAAAAAAAATAATATTATTACCAGATAGGCAGCTTTTA
TATCAAAACTGTAATCAGAGATTTGCTAAGCTAGCAACTAATGGAGAATTAGTAGATGAAATAATTAAAATAAAGCCATA
TTATGATCACATTAGCATTTCAGCGAAAAAGGCGCTAGGCATTAATGAGATAATCTCATATCTTAATCAAGAACTAACAA
TTGAAGAAGCTATAACAATAGCTCAGCAAAAAATTAGACAATATGCCAAAAGACAATTAACTTGGTTTAGAAATCAAACA
ATTAATGGATATACATTACATTATCAAAGTATGAGCGAGTTATACAAAACGCAAGTTAACGATGTGTTCTTTAATTAG

Upstream 100 bases:

>100_bases
TATCTTGTTCAATATTTAAGTTAGGATTCATACTATAGAAGTTATTGATTTAGTATACATTATATTATAATGTGCACTTA
TTATTGAAACTATATTAACA

Downstream 100 bases:

>100_bases
TTCTTGGTTACAAATAATCATCTACCCGTTAATAATATTATTAAATTATTTTTTTCCAAGACAATGCTTAACATGCCAAC
AGCTTATTCAAAGTGCTTTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDIFIIAGPTASGKSELAMLLAQKFNGVIINADSMQIYKEIPIITASPSISEKQQIDHYLYNYVSIFHSDINELNSFDN
ISQSKVNADYNNINLQQSITKHLTDRRYSVAKYVQEACKIIRSVIHALKLPIVVGGSGMYINALVYGIHHIPEITLEIRM
QVQDLYKKLTKQEFYQKLIDLDPISKNYIHSSDAQRMIRAYEVVLQTNKSIFSYHNSKLVSPLEGYNVKKIILLPDRQLL
YQNCNQRFAKLATNGELVDEIIKIKPYYDHISISAKKALGINEIISYLNQELTIEEAITIAQQKIRQYAKRQLTWFRNQT
INGYTLHYQSMSELYKTQVNDVFFN

Sequences:

>Translated_345_residues
MNDIFIIAGPTASGKSELAMLLAQKFNGVIINADSMQIYKEIPIITASPSISEKQQIDHYLYNYVSIFHSDINELNSFDN
ISQSKVNADYNNINLQQSITKHLTDRRYSVAKYVQEACKIIRSVIHALKLPIVVGGSGMYINALVYGIHHIPEITLEIRM
QVQDLYKKLTKQEFYQKLIDLDPISKNYIHSSDAQRMIRAYEVVLQTNKSIFSYHNSKLVSPLEGYNVKKIILLPDRQLL
YQNCNQRFAKLATNGELVDEIIKIKPYYDHISISAKKALGINEIISYLNQELTIEEAITIAQQKIRQYAKRQLTWFRNQT
INGYTLHYQSMSELYKTQVNDVFFN
>Mature_345_residues
MNDIFIIAGPTASGKSELAMLLAQKFNGVIINADSMQIYKEIPIITASPSISEKQQIDHYLYNYVSIFHSDINELNSFDN
ISQSKVNADYNNINLQQSITKHLTDRRYSVAKYVQEACKIIRSVIHALKLPIVVGGSGMYINALVYGIHHIPEITLEIRM
QVQDLYKKLTKQEFYQKLIDLDPISKNYIHSSDAQRMIRAYEVVLQTNKSIFSYHNSKLVSPLEGYNVKKIILLPDRQLL
YQNCNQRFAKLATNGELVDEIIKIKPYYDHISISAKKALGINEIISYLNQELTIEEAITIAQQKIRQYAKRQLTWFRNQT
INGYTLHYQSMSELYKTQVNDVFFN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Escherichia coli, GI1790613, Length=318, Percent_Identity=27.3584905660377, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=22.8758169934641, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=350, Percent_Identity=26.2857142857143, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR002648
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT; PF01745 IPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 39815; Mature: 39815

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIFIIAGPTASGKSELAMLLAQKFNGVIINADSMQIYKEIPIITASPSISEKQQIDHY
CCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCEEECCCCCCHHHHHHHH
LYNYVSIFHSDINELNSFDNISQSKVNADYNNINLQQSITKHLTDRRYSVAKYVQEACKI
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IRSVIHALKLPIVVGGSGMYINALVYGIHHIPEITLEIRMQVQDLYKKLTKQEFYQKLID
HHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPISKNYIHSSDAQRMIRAYEVVLQTNKSIFSYHNSKLVSPLEGYNVKKIILLPDRQLL
CCCCCHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHH
YQNCNQRFAKLATNGELVDEIIKIKPYYDHISISAKKALGINEIISYLNQELTIEEAITI
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECHHHHCCHHHHHHHHCCCCHHHHHHHH
AQQKIRQYAKRQLTWFRNQTINGYTLHYQSMSELYKTQVNDVFFN
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDIFIIAGPTASGKSELAMLLAQKFNGVIINADSMQIYKEIPIITASPSISEKQQIDHY
CCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCEEECCCCCCHHHHHHHH
LYNYVSIFHSDINELNSFDNISQSKVNADYNNINLQQSITKHLTDRRYSVAKYVQEACKI
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IRSVIHALKLPIVVGGSGMYINALVYGIHHIPEITLEIRMQVQDLYKKLTKQEFYQKLID
HHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPISKNYIHSSDAQRMIRAYEVVLQTNKSIFSYHNSKLVSPLEGYNVKKIILLPDRQLL
CCCCCHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHH
YQNCNQRFAKLATNGELVDEIIKIKPYYDHISISAKKALGINEIISYLNQELTIEEAITI
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECHHHHCCHHHHHHHHCCCCHHHHHHHH
AQQKIRQYAKRQLTWFRNQTINGYTLHYQSMSELYKTQVNDVFFN
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA