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The map label for this gene is grpE [H]

Identifier: 189184293

GI number: 189184293

Start: 1414902

End: 1415498

Strand: Direct

Name: grpE [H]

Synonym: OTT_1386

Alternate gene names: 189184293

Gene position: 1414902-1415498 (Clockwise)

Preceding gene: 189184291

Following gene: 189184299

Centisome position: 70.43

GC content: 29.65

Gene sequence:

>597_bases
ATGCAGGACAATCACACAAACCAACATAATAAAGATGCATCAGGTTATGTTGTGAATCAAGATAATGCAGATTCTCAGCA
AGTTGATAAAAAATCATCTAATCAAGAAGTACCAAATGATGATATAATTAATAACAAAGATAATGAAATAGCTCAGCTTA
ATAATGATTTGTTAAGAGCGATTGCAGAAAATGACAATACTATAAAAAGATACGAGCGCCAACTGCAAGAGGTAAAAGAA
TATGCTATATTTAACTTCGCTAAAGATATGCTTTCAGTTTTAGACGATTTAAGTCTTGCTTTAAGTAATATGGAACAGCA
ACTTGATAATAGCAATAACCAAGAAAACAACAAAATTAAAAATGCTATTACAGGTATAGAAATGACTCAAAAGAAATTTG
GATCCATTTTAAGTCAATATGGTATTCAAAAATTCGAACCTAAAACAGGAGAACCTTTTGATTCTAATATTCATCATGTT
CTTTCATTGGTTAAAGATATCAAATATGCTAAAGGTACGATAGTAAATGTTATGCAAATTGGATATAAGTTAAAAGATCG
CTTACTAAGACCTGCTATAGTATCTGTAGCAGAATAA

Upstream 100 bases:

>100_bases
AACATGGAATTAAGAAATATATTATAGATGAAGCTTTTTAGCAAATAGAATAAAAATCAGTTATATTATTTAAATTTAAT
AGTTATTAGGAACAAAAACT

Downstream 100 bases:

>100_bases
AAGTAACACAGAAAGTAAAAAAACTAGTTATATTACTCTAGCTTTAGCGCTGTGTTATATTAGAAAGCTATACTCTTAAT
TAATTGTATATTTAGTGCAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVPNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
LSLVKDIKYAKGTIVNVMQIGYKLKDRLLRPAIVSVAE

Sequences:

>Translated_198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVPNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
LSLVKDIKYAKGTIVNVMQIGYKLKDRLLRPAIVSVAE
>Mature_198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVPNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
LSLVKDIKYAKGTIVNVMQIGYKLKDRLLRPAIVSVAE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=138, Percent_Identity=32.6086956521739, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI40255109, Length=154, Percent_Identity=27.9220779220779, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17552458, Length=140, Percent_Identity=31.4285714285714, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=154, Percent_Identity=32.4675324675325, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653432, Length=148, Percent_Identity=30.4054054054054, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22510; Mature: 22510

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVPNDDIINNKDNEIAQLNNDLLRA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAENDNTIKRYERQLQEVKEYAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHVLSLVKDIKYAKGTIVNVMQI
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GYKLKDRLLRPAIVSVAE
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVPNDDIINNKDNEIAQLNNDLLRA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAENDNTIKRYERQLQEVKEYAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHVLSLVKDIKYAKGTIVNVMQI
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GYKLKDRLLRPAIVSVAE
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA