Click here to switch to the map view.

The map label for this gene is rph [H]

Identifier: 189184292

GI number: 189184292

Start: 1414017

End: 1414772

Strand: Reverse

Name: rph [H]

Synonym: OTT_1385

Alternate gene names: 189184292

Gene position: 1414772-1414017 (Counterclockwise)

Preceding gene: 189184294

Following gene: 189184290

Centisome position: 70.42

GC content: 35.05

Gene sequence:

>756_bases
ATGCGGTTATGTAGTAGAAAAGATAGTGAAATGCGCCCTATTAGCATAGAACTTGGGACAATGCTTAATAGTAAAGGGTC
ATGTTTGATTAAAATAGGTAATACACATGTAATATGTAGTGCTACTGTTGAGGAGTCTTTGCCTTTATTTCTCAAGAATA
AAAAGCAAGGTGGTTGGGTAACAGCAGAATATAGTATGTTACCAGGTTCTTCATTGCAGCGTGTTAAACGAGAAGGCATT
CAAGGAAAATCTGGCCGTACTCAAGAAATTCAAAGGTTAATCTCACGTGCTATGAGAGCAGCTGTAGATTTAAAGCTTTT
AGGGGAAAGGCAAATTTTAATAGATTGTGATGTTATTAATGCAGATGGTGGCACACGTGCTGCATCGATAACTGGTGGTT
ATGTTGCAATGTGTTTGGCTGTTAATAAGTTAATGCAAGAAAAAAATCTTGCAGTGTATCCTATCTTATATCAAGTAGCA
GCTATATCTTGTGGTATATCTAATGGTAGAGTGATTGTTGATCTAGATTATCAAGAAGATAGCTGTGCAGAAGTAGATGC
TAATTTTGTTTTTCGGCGTGCAGGAACAATTATAGAGATTGTAGAAATACAAGTTGCAGCAGAAAAAAAGGCTTTTGTAG
ATGAACAAGTAATACAAATGCTAAAGCTTGCTCAAAAAGCTATCAATCATATATTTGATATTCAAAATCAATCCTTGCTT
AAGTTAACCAAAGTTCGATATAATAAGGAGTGTTAA

Upstream 100 bases:

>100_bases
AAATAATATAACTGATTTTTATTCTATTTGCTAAAAAGCTTCATCTATAATATATTTCTTAATTCCATGTTTTCAAGTAA
TAAATTGGTATAAATCTAGT

Downstream 100 bases:

>100_bases
AGAGCAGAAAGAGAATATGAATAAATTCTTCTATATTTAGAGCAATTAGTATCTGTAGTTTCTGTGCAAACTTCTTTGCT
GCAGTAAAGTTGTTTAATTA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRLCSRKDSEMRPISIELGTMLNSKGSCLIKIGNTHVICSATVEESLPLFLKNKKQGGWVTAEYSMLPGSSLQRVKREGI
QGKSGRTQEIQRLISRAMRAAVDLKLLGERQILIDCDVINADGGTRAASITGGYVAMCLAVNKLMQEKNLAVYPILYQVA
AISCGISNGRVIVDLDYQEDSCAEVDANFVFRRAGTIIEIVEIQVAAEKKAFVDEQVIQMLKLAQKAINHIFDIQNQSLL
KLTKVRYNKEC

Sequences:

>Translated_251_residues
MRLCSRKDSEMRPISIELGTMLNSKGSCLIKIGNTHVICSATVEESLPLFLKNKKQGGWVTAEYSMLPGSSLQRVKREGI
QGKSGRTQEIQRLISRAMRAAVDLKLLGERQILIDCDVINADGGTRAASITGGYVAMCLAVNKLMQEKNLAVYPILYQVA
AISCGISNGRVIVDLDYQEDSCAEVDANFVFRRAGTIIEIVEIQVAAEKKAFVDEQVIQMLKLAQKAINHIFDIQNQSLL
KLTKVRYNKEC
>Mature_251_residues
MRLCSRKDSEMRPISIELGTMLNSKGSCLIKIGNTHVICSATVEESLPLFLKNKKQGGWVTAEYSMLPGSSLQRVKREGI
QGKSGRTQEIQRLISRAMRAAVDLKLLGERQILIDCDVINADGGTRAASITGGYVAMCLAVNKLMQEKNLAVYPILYQVA
AISCGISNGRVIVDLDYQEDSCAEVDANFVFRRAGTIIEIVEIQVAAEKKAFVDEQVIQMLKLAQKAINHIFDIQNQSLL
KLTKVRYNKEC

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=214, Percent_Identity=49.5327102803738, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI71981632, Length=184, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27784; Mature: 27784

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLCSRKDSEMRPISIELGTMLNSKGSCLIKIGNTHVICSATVEESLPLFLKNKKQGGWV
CCCCCCCCCCCCEEEEEECCEECCCCCEEEEECCEEEEEEECCCCCCCEEEECCCCCCEE
TAEYSMLPGSSLQRVKREGIQGKSGRTQEIQRLISRAMRAAVDLKLLGERQILIDCDVIN
EEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCEEEEEEEEEEE
ADGGTRAASITGGYVAMCLAVNKLMQEKNLAVYPILYQVAAISCGISNGRVIVDLDYQED
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCC
SCAEVDANFVFRRAGTIIEIVEIQVAAEKKAFVDEQVIQMLKLAQKAINHIFDIQNQSLL
CCHHCCHHHEEHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLTKVRYNKEC
HHHHHHCCCCC
>Mature Secondary Structure
MRLCSRKDSEMRPISIELGTMLNSKGSCLIKIGNTHVICSATVEESLPLFLKNKKQGGWV
CCCCCCCCCCCCEEEEEECCEECCCCCEEEEECCEEEEEEECCCCCCCEEEECCCCCCEE
TAEYSMLPGSSLQRVKREGIQGKSGRTQEIQRLISRAMRAAVDLKLLGERQILIDCDVIN
EEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCEEEEEEEEEEE
ADGGTRAASITGGYVAMCLAVNKLMQEKNLAVYPILYQVAAISCGISNGRVIVDLDYQED
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCC
SCAEVDANFVFRRAGTIIEIVEIQVAAEKKAFVDEQVIQMLKLAQKAINHIFDIQNQSLL
CCHHCCHHHEEHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLTKVRYNKEC
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA