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The map label for this gene is greA [H]

Identifier: 189184202

GI number: 189184202

Start: 1313305

End: 1313784

Strand: Direct

Name: greA [H]

Synonym: OTT_1295

Alternate gene names: 189184202

Gene position: 1313305-1313784 (Clockwise)

Preceding gene: 189184201

Following gene: 189184203

Centisome position: 65.37

GC content: 31.46

Gene sequence:

>480_bases
ATGGCAAGATTTCCGATTACTATAAAAGGTTTTCATAAGCTTGAGCAAGAACTTAAGCATTTAAAGTATGTTGAGCGGCT
TAAGATTACAACAGACATATCTACTGCTAGAGAGTTTGGAGATTTATCTGAAAATGCTGAGTATAAGGCAGCAAAGGAAA
GGCAGTTATTAAATGATAAAAAAATTTACGACTTAGAAAATAAATTAGCAAATGCAGAAGTTATTGAAATAACTAAGATT
AATAGTAACTCAGTTAAGTTTGGTGCGCGAGTAGTATTATTGGATCGAGATACTGAAAAGGAAGTAGTATATCAAATTGT
TGGAGAGTATGAAGCAGATATTACACAAAACCTTATTTCTATAGCATCTCCAATAGCACAAGCATTGATTGGAAAAAAGG
CAGGAGATATTATTGAAGTTATTACTCCTAAAGGTGGAAGATTTTATGAGCTACTAAAAGTTCAATATGTTGATTTTTAA

Upstream 100 bases:

>100_bases
ATTTACCATACAGTATTTTAATGATAAAATTGCTTTTGATAATTTTAGTTAGTGATATATACTTCTAAAGTAACTAAATA
ATTATTTAAAATAAAAAGAT

Downstream 100 bases:

>100_bases
AGTAATGCATTTAATTCATAGTTACATATAAAATAAAGAAATTGTTATGGATAATGAAATAGATAATTTACCAATATCAG
CTTCAGATACAGATAATGGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKI
NSNSVKFGARVVLLDRDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF

Sequences:

>Translated_159_residues
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKI
NSNSVKFGARVVLLDRDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
>Mature_158_residues
ARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKIN
SNSVKFGARVVLLDRDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=48.7341772151899, Blast_Score=147, Evalue=2e-37,
Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=34.4155844155844, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18105; Mature: 17974

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDK
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
KIYDLENKLANAEVIEITKINSNSVKFGARVVLLDRDTEKEVVYQIVGEYEADITQNLIS
HHHHHHHHHCCCEEEEEEECCCCCEEECEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
IASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
ARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDK
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
KIYDLENKLANAEVIEITKINSNSVKFGARVVLLDRDTEKEVVYQIVGEYEADITQNLIS
HHHHHHHHHCCCEEEEEEECCCCCEEECEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
IASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA