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The map label for this gene is engD [H]

Identifier: 189184107

GI number: 189184107

Start: 1229198

End: 1230298

Strand: Direct

Name: engD [H]

Synonym: OTT_1200

Alternate gene names: 189184107

Gene position: 1229198-1230298 (Clockwise)

Preceding gene: 189184106

Following gene: 189184108

Centisome position: 61.18

GC content: 33.97

Gene sequence:

>1101_bases
ATGGAACTTAGATATGGAATAGTTGGATTGCCTAACGTAGGAAAATCAACATTATTTAATGCTTTAACTGCTACTCAATC
TGCTAATGTTGGAAATTATCCATTTTGTACAATTGAGCCTAATATTGGTATTGTAGCTGTTCCAGATCACAGGCTGCAAA
AATTAGCTGATATTGCTGGTTCGCAAAGCATTATTCCAACCTATGTTAAATTCGTTGATATTGCAGGCTTAGTAAAAGGT
GCTAGCTCTGGAGAAGGGCTAGGTAATAAATTTTTATCTCACATTAGGGAGGTAGATGCAATTATTCATGTTTTAAGATG
CTTTGATATTTCAGATATTACTCATGTATATGGTAGTATTGATCCAGTTCGTGATGCTGAGATAATAGAGCTAGAGCTTA
TTTTAGCAGATCTTGAGTCTATAGAGAAAAGATTGCCTGCCTTGGAAAAAAAGTCTAGGGGGGACGATCAAAAAGCTAAA
GAAGAAATAGAACTGTTATATCATGCACAACAAATTTTGCGTAATGATCAACCAGTAAGAAAGCTGCCATTATATCGCGA
AAAAAGTCAAAAATTTGATCAGTTGCAGTTGCTTACTACTAAGCCAGTAATATATGTTTGCAATGTGCTAGAACATGAAG
CAATATCTGGTAATGCTTTGTGTAAAGCTGTGCAAGAAAGCGATATATTAGGTCAATCAGATATGATTATTATTTCATCA
AGTATTGAATCTGAAATTGCTAGTTTAAGCACTTTAGAAGAAAAAAAAGATTATCTGAATATTTGGGGACTCCAAGAAAC
TGGGTTGAATAAGATCATTAAATCTTGCTATAGGTTATTTGATTTAAATAGTTATTTTACCGTTGGACCTAAAGAAGCTC
GAGCTTGGACTATTGTTAAAGGCACATTAGCTCCTCAAGCTGCAGGAATAATTCATACTGATTTTGAAAGAGGATTCATT
AAGGCAGAGGTTATATCTTATGAAGATTATATAAAATATAATGGTGAATCTAAAGCTAAAGAGCACGGGCGTTTGCGAAT
TGAAGGCAAAGACTATGTGGTGCAAGATGGTGATGTTATTCACTTTAGATTTAATGTTTAA

Upstream 100 bases:

>100_bases
GTTATATTCTATAGCAAGAAAAATTGCTGAAAATTTTGAGCTAGCAGTTCAAAAACAATTCAGTGAATTTATGTTTAAGA
TAAAAAATTGATTGATAAAA

Downstream 100 bases:

>100_bases
TCATTAATCTCTTTGCTTATAGTAAATTTTAGGCTGAATTAAGCCTTTTATATTTTACCTTTATTATATGAACTGGCGTT
AATAACGCCAGTTTAGGATA

Product: GTP-binding protein, Era/Obg family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MELRYGIVGLPNVGKSTLFNALTATQSANVGNYPFCTIEPNIGIVAVPDHRLQKLADIAGSQSIIPTYVKFVDIAGLVKG
ASSGEGLGNKFLSHIREVDAIIHVLRCFDISDITHVYGSIDPVRDAEIIELELILADLESIEKRLPALEKKSRGDDQKAK
EEIELLYHAQQILRNDQPVRKLPLYREKSQKFDQLQLLTTKPVIYVCNVLEHEAISGNALCKAVQESDILGQSDMIIISS
SIESEIASLSTLEEKKDYLNIWGLQETGLNKIIKSCYRLFDLNSYFTVGPKEARAWTIVKGTLAPQAAGIIHTDFERGFI
KAEVISYEDYIKYNGESKAKEHGRLRIEGKDYVVQDGDVIHFRFNV

Sequences:

>Translated_366_residues
MELRYGIVGLPNVGKSTLFNALTATQSANVGNYPFCTIEPNIGIVAVPDHRLQKLADIAGSQSIIPTYVKFVDIAGLVKG
ASSGEGLGNKFLSHIREVDAIIHVLRCFDISDITHVYGSIDPVRDAEIIELELILADLESIEKRLPALEKKSRGDDQKAK
EEIELLYHAQQILRNDQPVRKLPLYREKSQKFDQLQLLTTKPVIYVCNVLEHEAISGNALCKAVQESDILGQSDMIIISS
SIESEIASLSTLEEKKDYLNIWGLQETGLNKIIKSCYRLFDLNSYFTVGPKEARAWTIVKGTLAPQAAGIIHTDFERGFI
KAEVISYEDYIKYNGESKAKEHGRLRIEGKDYVVQDGDVIHFRFNV
>Mature_366_residues
MELRYGIVGLPNVGKSTLFNALTATQSANVGNYPFCTIEPNIGIVAVPDHRLQKLADIAGSQSIIPTYVKFVDIAGLVKG
ASSGEGLGNKFLSHIREVDAIIHVLRCFDISDITHVYGSIDPVRDAEIIELELILADLESIEKRLPALEKKSRGDDQKAK
EEIELLYHAQQILRNDQPVRKLPLYREKSQKFDQLQLLTTKPVIYVCNVLEHEAISGNALCKAVQESDILGQSDMIIISS
SIESEIASLSTLEEKKDYLNIWGLQETGLNKIIKSCYRLFDLNSYFTVGPKEARAWTIVKGTLAPQAAGIIHTDFERGFI
KAEVISYEDYIKYNGESKAKEHGRLRIEGKDYVVQDGDVIHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=45.4054054054054, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI58761502, Length=219, Percent_Identity=38.3561643835616, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1787454, Length=366, Percent_Identity=50, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789574, Length=142, Percent_Identity=33.8028169014084, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=43.8692098092643, Blast_Score=284, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=42.972972972973, Blast_Score=274, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321773, Length=378, Percent_Identity=35.978835978836, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321649, Length=162, Percent_Identity=38.2716049382716, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640873, Length=373, Percent_Identity=44.2359249329759, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640877, Length=373, Percent_Identity=44.2359249329759, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640875, Length=373, Percent_Identity=44.2359249329759, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640879, Length=331, Percent_Identity=41.9939577039275, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40908; Mature: 40908

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRYGIVGLPNVGKSTLFNALTATQSANVGNYPFCTIEPNIGIVAVPDHRLQKLADIAG
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCC
SQSIIPTYVKFVDIAGLVKGASSGEGLGNKFLSHIREVDAIIHVLRCFDISDITHVYGSI
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
DPVRDAEIIELELILADLESIEKRLPALEKKSRGDDQKAKEEIELLYHAQQILRNDQPVR
CCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
KLPLYREKSQKFDQLQLLTTKPVIYVCNVLEHEAISGNALCKAVQESDILGQSDMIIISS
HCCCHHHHHCCHHHEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEC
SIESEIASLSTLEEKKDYLNIWGLQETGLNKIIKSCYRLFDLNSYFTVGPKEARAWTIVK
CHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
GTLAPQAAGIIHTDFERGFIKAEVISYEDYIKYNGESKAKEHGRLRIEGKDYVVQDGDVI
ECCCCCCCCEEEECCCCCEEEEEEECHHHHEEECCCCCHHHCCEEEECCCCEEEECCCEE
HFRFNV
EEEECC
>Mature Secondary Structure
MELRYGIVGLPNVGKSTLFNALTATQSANVGNYPFCTIEPNIGIVAVPDHRLQKLADIAG
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCC
SQSIIPTYVKFVDIAGLVKGASSGEGLGNKFLSHIREVDAIIHVLRCFDISDITHVYGSI
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
DPVRDAEIIELELILADLESIEKRLPALEKKSRGDDQKAKEEIELLYHAQQILRNDQPVR
CCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
KLPLYREKSQKFDQLQLLTTKPVIYVCNVLEHEAISGNALCKAVQESDILGQSDMIIISS
HCCCHHHHHCCHHHEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEC
SIESEIASLSTLEEKKDYLNIWGLQETGLNKIIKSCYRLFDLNSYFTVGPKEARAWTIVK
CHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
GTLAPQAAGIIHTDFERGFIKAEVISYEDYIKYNGESKAKEHGRLRIEGKDYVVQDGDVI
ECCCCCCCCEEEECCCCCEEEEEEECHHHHEEECCCCCHHHCCEEEECCCCEEEECCCEE
HFRFNV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]