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The map label for this gene is xthA2 [H]

Identifier: 189184108

GI number: 189184108

Start: 1231669

End: 1232463

Strand: Direct

Name: xthA2 [H]

Synonym: OTT_1201

Alternate gene names: 189184108

Gene position: 1231669-1232463 (Clockwise)

Preceding gene: 189184107

Following gene: 189184109

Centisome position: 61.31

GC content: 30.06

Gene sequence:

>795_bases
ATGAGCTGTAAACTAAAAATTTTAACTTGGAATATTAATTCTATTAGGGCAAGAGCATATCAACTAAACCAACTTATTTC
TGATACACAGCCAGATATTATTTTATTACAGGAAATCAAATGCGAAAGCCATAACTTTCCTTTTCAAGAAATTAATGAGT
CACATAAATATAATTTCAGTATTAATGGCCAGAAGTCATACAATGGTGTTGCAATATTATCAAAATTCCCTCTAGAAGAT
ATAAAATTTAGTTTTTTGGGCAACCCTTGCCCAGATCAAGCAAGATTCATTGAAGCAAATTGTTTAACTGAAGTAGGATA
TGTTAAAGTTATTTCAATTTATGTTCCAAATGGAGGAGAAGTCAATAGTGATAAATTTCAAATTAAGCTACTTTTCATTG
ATGAATTTATTAAGTACTTACATAGTAGTAAAAATTTAGGTGAATCAATAATATTGGGTGGAGATTTTAATGTTGCCCCA
TACGATAATGATGTTTATTCTGCTATAGAGTTACAAAATACAACTTGTTTTACCTTGCCTGAAAAGCAATATATACGTAA
GTTAATAAATCATAATTTTATAGATATTTATCGATTATTTCACCAGCGACAAAAAAAATTTACGTGGTGGGATTATCGAG
CTGGAGCATTTGAGCGTAACTTAGGAATGCGTATAGATTATATACTTTGTACAATCTCTATTGCTCTAAAAGCTACAGAT
TGCTATATTGATTATCATACTAGAAACAATCATAGAGCTTCAGATCATGCTCCTGTCATTGCTAGTTTTGAGTGA

Upstream 100 bases:

>100_bases
AGATAATAACCCTTTATGCAGAAATACATTATTTGTTTTACAGATGATACTAACTTACATCTTGTAAAAAGTTTTGGTTA
CAAATATACTAAATATTATT

Downstream 100 bases:

>100_bases
TTTCAAATTCTTGTTAGTTCTATGAAAGTTGATATTGAATCAATTATATTATTACTGCAGTTAAAAATAATAGATCTAAA
TTAAGCATGCTCAATACTTA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSCKLKILTWNINSIRARAYQLNQLISDTQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVAILSKFPLED
IKFSFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDEFIKYLHSSKNLGESIILGGDFNVAP
YDNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCTISIALKATD
CYIDYHTRNNHRASDHAPVIASFE

Sequences:

>Translated_264_residues
MSCKLKILTWNINSIRARAYQLNQLISDTQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVAILSKFPLED
IKFSFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDEFIKYLHSSKNLGESIILGGDFNVAP
YDNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCTISIALKATD
CYIDYHTRNNHRASDHAPVIASFE
>Mature_263_residues
SCKLKILTWNINSIRARAYQLNQLISDTQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVAILSKFPLEDI
KFSFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDEFIKYLHSSKNLGESIILGGDFNVAPY
DNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCTISIALKATDC
YIDYHTRNNHRASDHAPVIASFE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=30.7116104868914, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=30.7116104868914, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=30.7116104868914, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=33.7078651685393, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=264, Percent_Identity=28.4090909090909, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30661; Mature: 30530

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCKLKILTWNINSIRARAYQLNQLISDTQPDIILLQEIKCESHNFPFQEINESHKYNFS
CCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEEE
INGQKSYNGVAILSKFPLEDIKFSFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGE
ECCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHEEEEHHHHCCCCEEEEEEEEECCCCC
VNSDKFQIKLLFIDEFIKYLHSSKNLGESIILGGDFNVAPYDNDVYSAIELQNTTCFTLP
CCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCEEEEEECCCEEEECC
EKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCTISIALKATD
HHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCEECCCCCEEEEEEEEEEEEEEEEE
CYIDYHTRNNHRASDHAPVIASFE
EEEEEECCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SCKLKILTWNINSIRARAYQLNQLISDTQPDIILLQEIKCESHNFPFQEINESHKYNFS
CEEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEEE
INGQKSYNGVAILSKFPLEDIKFSFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGE
ECCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHEEEEHHHHCCCCEEEEEEEEECCCCC
VNSDKFQIKLLFIDEFIKYLHSSKNLGESIILGGDFNVAPYDNDVYSAIELQNTTCFTLP
CCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCEEEEEECCCEEEECC
EKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCTISIALKATD
HHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCEECCCCCEEEEEEEEEEEEEEEEE
CYIDYHTRNNHRASDHAPVIASFE
EEEEEECCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]