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The map label for this gene is mjaIIIM [H]

Identifier: 189183937

GI number: 189183937

Start: 1071590

End: 1072054

Strand: Direct

Name: mjaIIIM [H]

Synonym: OTT_1030

Alternate gene names: 189183937

Gene position: 1071590-1072054 (Clockwise)

Preceding gene: 189183936

Following gene: 189183943

Centisome position: 53.34

GC content: 30.11

Gene sequence:

>465_bases
TTGCTATATCTTAATAAATATTCTTTTAGGGGAATTTATAGAGTCTACAAAAATGGACAATCTGCTCAAACATTTTCTGG
TGAATGCTACATTAAACTCCATATTGCATCTAGAATAAACCAATGCAGCAACCTTTTACATGGTGTATCAATTTATGCTA
CAGATTTTTCATTTATAGAGCCTCAACAAAATGACTTTGTTTATTTCGATCCTCCTTATCATAAATCTGGAGAAAGGTTC
TACACTAGACTTCCATTTGATGAAAAAGATCAAATTAGATTACGGGATTTTGTTTATGAACTACACAATAAAGGTGTTAA
GATTATGATCTCAAATAATAATACTGCCTTTATTAGAGACTTATACAAAGATTTTAATATCAATACCGTTACAGTCGTAT
ACTCAATCAATGAACAACGCAATCCTGTTAATGAGCTAATCATTACTAACTATAAAACTTGCTAG

Upstream 100 bases:

>100_bases
TAAGAACGAAATAAGAAAAGTTACTCCTCAATTCAAAGATATTAGCATGGCTGTAGCACACGTAATGCAGTTTATCTGAT
TGTACCTATTTCCTACGTTA

Downstream 100 bases:

>100_bases
TTATTAAATGTGAAACCGCAATCCAAAACGCGTATGAATATGACTTGATATATGGTCAACCATTATGCTACATTCAATAT
CTAAATATATATCTCTTAAT

Product: N6 adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSNLLHGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERF
YTRLPFDEKDQIRLRDFVYELHNKGVKIMISNNNTAFIRDLYKDFNINTVTVVYSINEQRNPVNELIITNYKTC

Sequences:

>Translated_154_residues
MLYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSNLLHGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERF
YTRLPFDEKDQIRLRDFVYELHNKGVKIMISNNNTAFIRDLYKDFNINTVTVVYSINEQRNPVNELIITNYKTC
>Mature_154_residues
MLYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSNLLHGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERF
YTRLPFDEKDQIRLRDFVYELHNKGVKIMISNNNTAFIRDLYKDFNINTVTVVYSINEQRNPVNELIITNYKTC

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 18271; Mature: 18271

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSNLLHGVSIYATDFSFIE
CEEECCCCCHHHEEECCCCCCCCEECCEEEEEEEHHHHHHHHHHHHCCEEEEEECCEEEC
PQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVYELHNKGVKIMISNNNTAFIRD
CCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEH
LYKDFNINTVTVVYSINEQRNPVNELIITNYKTC
HHHHCCCCEEEEEEEECCCCCCCHHEEEECCCCC
>Mature Secondary Structure
MLYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSNLLHGVSIYATDFSFIE
CEEECCCCCHHHEEECCCCCCCCEECCEEEEEEEHHHHHHHHHHHHCCEEEEEECCEEEC
PQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVYELHNKGVKIMISNNNTAFIRD
CCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEH
LYKDFNINTVTVVYSINEQRNPVNELIITNYKTC
HHHHCCCCEEEEEEEECCCCCCCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]