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The map label for this gene is truA [H]

Identifier: 189183835

GI number: 189183835

Start: 968154

End: 969005

Strand: Reverse

Name: truA [H]

Synonym: OTT_0928

Alternate gene names: 189183835

Gene position: 969005-968154 (Counterclockwise)

Preceding gene: 189183844

Following gene: 189183834

Centisome position: 48.23

GC content: 32.63

Gene sequence:

>852_bases
ATGTTGCGTTACAAAGCTATTGTTGAGTATGATGGCACTAATTTTGTTGGATGGCAACGGCAACAAAATGGATTATCAAT
ACAGCAATTACTTGAAGATAAAATATCAACATTTACTAAACAAACTGTCAATCTTATTGCTGCTGGTCGCACTGATGCTG
GTGTACACGCCTTAGGTCAAGTAGTACATTTTGACTTAATCTCTCCTAATAATAGCAAAGATCTTGCTTGTATTAACAAA
GAAACAGATAACAAAGAAGTAAGCAAGCAGAATAATACTACTACTACAATTGACAGCTTAAAAATGCTTCCATGCCGATA
TAATGCTTACAAGTTAATGTCAGCAGTTAATTATTTATTAAAACCTCACCGTATAATATTAACAAGTTGTGAGATAACTA
CTTTACAATTTCATGCTAGATTTTCTGCTAAAGCTAGACATTATAAATATAGAATTATAAACCGCGCAGTTCCTTCAGTT
ATCGAACAAAATCGCACTTGGTGGATTAAGACTCCACTAAATGTTATTGATATGATTGATGCATCTCAGCATCTGATTGG
CAAGCATGATTTTACATCTTTTAGATCTTCTGCATGCCAAGCAAAATCTCCATTAAAAACTTTAACTAAAATTGAAGTAG
ATACTACTAATTATCCAGAAATACAAATTTATTTTTCAGCTCCATCATTTTTACACCACATGGTAAGAAATATAGTGGGT
ACATTAGTTTATATTGGTTTATGCAAAATATCTCCAGCTGCTATCAAAACAATCTTATTTGCAAAAAACAGAGCAATGGC
TGGACCTACAGCACCATCTTCAGGTTTATATTTTGTTAAGGTAGATTACTAG

Upstream 100 bases:

>100_bases
CATAAAATGCTCTATAACGCAACAAAATTAAAGGTTTTTTATTTACTTTATTTATATTTGTTATATATATGAATATTATT
TTAAATATTTCATATTTCTA

Downstream 100 bases:

>100_bases
TTTGCGTATTAAAGATGCTCTTGCGATCGGCAACAACTTACTATCACAGTATGAAATACCAAATTCTGCTTTTGAGTCTA
GGCTACTGCTAGCTCATATA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MLRYKAIVEYDGTNFVGWQRQQNGLSIQQLLEDKISTFTKQTVNLIAAGRTDAGVHALGQVVHFDLISPNNSKDLACINK
ETDNKEVSKQNNTTTTIDSLKMLPCRYNAYKLMSAVNYLLKPHRIILTSCEITTLQFHARFSAKARHYKYRIINRAVPSV
IEQNRTWWIKTPLNVIDMIDASQHLIGKHDFTSFRSSACQAKSPLKTLTKIEVDTTNYPEIQIYFSAPSFLHHMVRNIVG
TLVYIGLCKISPAAIKTILFAKNRAMAGPTAPSSGLYFVKVDY

Sequences:

>Translated_283_residues
MLRYKAIVEYDGTNFVGWQRQQNGLSIQQLLEDKISTFTKQTVNLIAAGRTDAGVHALGQVVHFDLISPNNSKDLACINK
ETDNKEVSKQNNTTTTIDSLKMLPCRYNAYKLMSAVNYLLKPHRIILTSCEITTLQFHARFSAKARHYKYRIINRAVPSV
IEQNRTWWIKTPLNVIDMIDASQHLIGKHDFTSFRSSACQAKSPLKTLTKIEVDTTNYPEIQIYFSAPSFLHHMVRNIVG
TLVYIGLCKISPAAIKTILFAKNRAMAGPTAPSSGLYFVKVDY
>Mature_283_residues
MLRYKAIVEYDGTNFVGWQRQQNGLSIQQLLEDKISTFTKQTVNLIAAGRTDAGVHALGQVVHFDLISPNNSKDLACINK
ETDNKEVSKQNNTTTTIDSLKMLPCRYNAYKLMSAVNYLLKPHRIILTSCEITTLQFHARFSAKARHYKYRIINRAVPSV
IEQNRTWWIKTPLNVIDMIDASQHLIGKHDFTSFRSSACQAKSPLKTLTKIEVDTTNYPEIQIYFSAPSFLHHMVRNIVG
TLVYIGLCKISPAAIKTILFAKNRAMAGPTAPSSGLYFVKVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=307, Percent_Identity=28.0130293159609, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1788657, Length=277, Percent_Identity=36.8231046931408, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31989; Mature: 31989

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYKAIVEYDGTNFVGWQRQQNGLSIQQLLEDKISTFTKQTVNLIAAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
VVHFDLISPNNSKDLACINKETDNKEVSKQNNTTTTIDSLKMLPCRYNAYKLMSAVNYLL
HHEEEEECCCCCCCEEEECCCCCCCHHHCCCCCEEEHHHEEECCCCCHHHHHHHHHHHHH
KPHRIILTSCEITTLQFHARFSAKARHYKYRIINRAVPSVIEQNRTWWIKTPLNVIDMID
CCCEEEEEECEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHC
ASQHLIGKHDFTSFRSSACQAKSPLKTLTKIEVDTTNYPEIQIYFSAPSFLHHMVRNIVG
CHHHHCCCCHHHHHHHHHHCCCCCHHHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHH
TLVYIGLCKISPAAIKTILFAKNRAMAGPTAPSSGLYFVKVDY
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MLRYKAIVEYDGTNFVGWQRQQNGLSIQQLLEDKISTFTKQTVNLIAAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
VVHFDLISPNNSKDLACINKETDNKEVSKQNNTTTTIDSLKMLPCRYNAYKLMSAVNYLL
HHEEEEECCCCCCCEEEECCCCCCCHHHCCCCCEEEHHHEEECCCCCHHHHHHHHHHHHH
KPHRIILTSCEITTLQFHARFSAKARHYKYRIINRAVPSVIEQNRTWWIKTPLNVIDMID
CCCEEEEEECEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHC
ASQHLIGKHDFTSFRSSACQAKSPLKTLTKIEVDTTNYPEIQIYFSAPSFLHHMVRNIVG
CHHHHCCCCHHHHHHHHHHCCCCCHHHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHH
TLVYIGLCKISPAAIKTILFAKNRAMAGPTAPSSGLYFVKVDY
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA