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The map label for this gene is hemK [H]

Identifier: 189183834

GI number: 189183834

Start: 967286

End: 968152

Strand: Reverse

Name: hemK [H]

Synonym: OTT_0927

Alternate gene names: 189183834

Gene position: 968152-967286 (Counterclockwise)

Preceding gene: 189183835

Following gene: 189183832

Centisome position: 48.19

GC content: 30.8

Gene sequence:

>867_bases
TTGCGTATTAAAGATGCTCTTGCGATCGGCAACAACTTACTATCACAGTATGAAATACCAAATTCTGCTTTTGAGTCTAG
GCTACTGCTAGCTCATATAATTGGTCAAAGCCAAGAATATATACTTTTTCATTCTGATCATTTGTTATTAAATAGTGACT
ACAATCAATTTTTAAAAATGATAAAATTACGCACTAAGTACTTACCTATTGCTTATTTGATAGGATATAAAGAATTTTAC
AGTCGGAATTTTATAGTTGATAAATCAGTATTAATTCCTAGACCAGATTCTGAAACTTTAATTGATGCAGTAGTCCAAGA
TTATAGGAAGATAGCTAACTACCAACCTTCAGCACCAATAAAAATCCTTGAATTGGGAGTTGGTAGTGGGTGCTTAATTA
TTACTCTACTTCTTGAGCTAAGTAATGCTATTGGAGTTGGATCAGACATTTCAATATCAGCTTTAAATATTGCTAGTCGT
AATCGCCAAAAATATAAATTGGAAAAGTCATTAAATTTAGTACAAAGTAACTGGTTCAGTGGATTAGATGTAGGTGAAAA
ATATGATATCATTATCGCAAATCCACCTTATGTATCTGATTCTGAATTAAGAATATTATCACGTGAAACATTGTTGCATG
AGCCACATATAGCTTTATTTAGTAATAATAATGGGCTTCAGTCATATCAAGAAATAGCACCTTTGATTCCTAGTTTTCTA
AATTCAAATGGAAGGTTATACTTAGAGTGTAGTTATAACAAAGCAGAAATGGTATGTGCGCTTTGCTTCAAAAGTGGCTT
AATACTTGAGAATAAATATTATGATTTTAATAGTTATTGCAGATGCTTAAAATTTAAATTGCAGTAA

Upstream 100 bases:

>100_bases
CCAGCTGCTATCAAAACAATCTTATTTGCAAAAAACAGAGCAATGGCTGGACCTACAGCACCATCTTCAGGTTTATATTT
TGTTAAGGTAGATTACTAGT

Downstream 100 bases:

>100_bases
TATACATGATATAATTAACGCTTATATCATTAGTTAAGCGCCATTGTTGCAATATAGGATTATATGATAATCCTTGAATT
TCTTTTAAAGTCATGTTATG

Product: possible protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRIKDALAIGNNLLSQYEIPNSAFESRLLLAHIIGQSQEYILFHSDHLLLNSDYNQFLKMIKLRTKYLPIAYLIGYKEFY
SRNFIVDKSVLIPRPDSETLIDAVVQDYRKIANYQPSAPIKILELGVGSGCLIITLLLELSNAIGVGSDISISALNIASR
NRQKYKLEKSLNLVQSNWFSGLDVGEKYDIIIANPPYVSDSELRILSRETLLHEPHIALFSNNNGLQSYQEIAPLIPSFL
NSNGRLYLECSYNKAEMVCALCFKSGLILENKYYDFNSYCRCLKFKLQ

Sequences:

>Translated_288_residues
MRIKDALAIGNNLLSQYEIPNSAFESRLLLAHIIGQSQEYILFHSDHLLLNSDYNQFLKMIKLRTKYLPIAYLIGYKEFY
SRNFIVDKSVLIPRPDSETLIDAVVQDYRKIANYQPSAPIKILELGVGSGCLIITLLLELSNAIGVGSDISISALNIASR
NRQKYKLEKSLNLVQSNWFSGLDVGEKYDIIIANPPYVSDSELRILSRETLLHEPHIALFSNNNGLQSYQEIAPLIPSFL
NSNGRLYLECSYNKAEMVCALCFKSGLILENKYYDFNSYCRCLKFKLQ
>Mature_288_residues
MRIKDALAIGNNLLSQYEIPNSAFESRLLLAHIIGQSQEYILFHSDHLLLNSDYNQFLKMIKLRTKYLPIAYLIGYKEFY
SRNFIVDKSVLIPRPDSETLIDAVVQDYRKIANYQPSAPIKILELGVGSGCLIITLLLELSNAIGVGSDISISALNIASR
NRQKYKLEKSLNLVQSNWFSGLDVGEKYDIIIANPPYVSDSELRILSRETLLHEPHIALFSNNNGLQSYQEIAPLIPSFL
NSNGRLYLECSYNKAEMVCALCFKSGLILENKYYDFNSYCRCLKFKLQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=262, Percent_Identity=26.3358778625954, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1787463, Length=257, Percent_Identity=29.5719844357977, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082085, Length=193, Percent_Identity=30.5699481865285, Blast_Score=85, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=160, Percent_Identity=26.875, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582226, Length=267, Percent_Identity=25.4681647940075, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32780; Mature: 32780

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKDALAIGNNLLSQYEIPNSAFESRLLLAHIIGQSQEYILFHSDHLLLNSDYNQFLKM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCHHHHHHH
IKLRTKYLPIAYLIGYKEFYSRNFIVDKSVLIPRPDSETLIDAVVQDYRKIANYQPSAPI
HHHHHHHCCHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCE
KILELGVGSGCLIITLLLELSNAIGVGSDISISALNIASRNRQKYKLEKSLNLVQSNWFS
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCC
GLDVGEKYDIIIANPPYVSDSELRILSRETLLHEPHIALFSNNNGLQSYQEIAPLIPSFL
CCCCCCCEEEEECCCCCCCCCHHEEHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
NSNGRLYLECSYNKAEMVCALCFKSGLILENKYYDFNSYCRCLKFKLQ
CCCCEEEEEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHEECC
>Mature Secondary Structure
MRIKDALAIGNNLLSQYEIPNSAFESRLLLAHIIGQSQEYILFHSDHLLLNSDYNQFLKM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCHHHHHHH
IKLRTKYLPIAYLIGYKEFYSRNFIVDKSVLIPRPDSETLIDAVVQDYRKIANYQPSAPI
HHHHHHHCCHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCE
KILELGVGSGCLIITLLLELSNAIGVGSDISISALNIASRNRQKYKLEKSLNLVQSNWFS
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCC
GLDVGEKYDIIIANPPYVSDSELRILSRETLLHEPHIALFSNNNGLQSYQEIAPLIPSFL
CCCCCCCEEEEECCCCCCCCCHHEEHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
NSNGRLYLECSYNKAEMVCALCFKSGLILENKYYDFNSYCRCLKFKLQ
CCCCEEEEEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA