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The map label for this gene is nuoF [H]

Identifier: 189183823

GI number: 189183823

Start: 957809

End: 959077

Strand: Direct

Name: nuoF [H]

Synonym: OTT_0916

Alternate gene names: 189183823

Gene position: 957809-959077 (Clockwise)

Preceding gene: 189183822

Following gene: 189183827

Centisome position: 47.68

GC content: 36.33

Gene sequence:

>1269_bases
ATGTTAAATATTGAGGATAAAATTTTTACCAATTTATCTGGTTCTATGGCAGTTGACCTTGCCAGCGCTAAAAAGCGCGG
TGACTGGCATGCAACGAAAGATTTGATATTAAATAGCAGAGAATGGATTATTGAAGAAATTAAAAAATCTGGCTTACGTG
GTCGAGGTGGAGCTGGATTTCCTACAGGTTTAAAATGGTCTTTTATGCCTAAAAACTCCGAAAAGGCAACTTATCTAGTT
GTTAATGCTGATGAATCAGAGCCAGGTACATGTAAAGATAGAGATATAATTCGTTATGAACCTCATAAATTAATTGAAGG
GTGCTTACTCGCAGCATATGCTATTGGAGCTCAAACATGTTATATATATATACGAGGTGAATATTATAATGAAGCTTTAA
TATTACAAAATGCAATTCAAGAGGCATATGATGCTAGGCTTATAGGGCTAAATTCATGCGGCACAGACTTTAACTTTAAT
CTTTATCTGCATAGAGGAGCTGGAGCATATATTTGTGGAGAGGAAACAGCTCTGCTTGAAAGCTTAGAAGGAAAAAAAGG
GATGCCGCGTTTAAAACCTCCATTTCCAGCTAATATTGGATTATATGGATGTCCTACTACTATTAATAATGTTGAATCTA
TAGCTGTAGTTCCAACAATACTCAGAAGAGGCGCAAAATGGTTCGCCAGCATTGGTAAACCAAACAATACAGGAACTAAA
ATTTTTAGCATTTCAGGGCATGTTAATAATCCTTGTAATATTGAAGAAGCAATGGGAGTTCCGCTTAAATATCTAATAGA
AAAATATGCTGGAGGAGTACGTGGTGGATGGGATAATCTTAAAGCTATTATTCCAGGTGGATCATCTGTTCCGCTGATTC
CTAAAAATATCTGCGATACAGTAGAAATGGATTTTGATAGCTTGAAATCAGTAGGCTCAGGATTAGGCACAGGCGGTATT
ATAGTAATGGATAATAATACTGATATCATTTATGCTATCGCCAGGCTAAGTAAGTTTTATATGCATGAATCATGTGGCCA
ATGTACACCATGCCGAGAAGGAACAGGCTGGATGTGGCGAATTATGATGAGACTAGTAAAAGGAAGTGCATCAGTAAGCG
ATATAGACATGCTATTAGATGTTACTAAGCAAATAGAAGGGAATACTATATGTGCATTAGGCGATGCAGCAGCTTGGCCT
ATTCAAGGGTTAATAAAGCATTTTAGGCATGAAATTGAAGATCGTATAAAAAACTTTAATATTCAATAA

Upstream 100 bases:

>100_bases
TCGAAGAAATTATCAATAATCTGCATGCTAAAGATTTACAAAAAAAATCTGAACTAAAACCTGATAATAAAACTGACTTA
TAACTCAAGAGCAATTATAA

Downstream 100 bases:

>100_bases
CATTACTGCTTAACTAAGAATTAAATTACTAAGAATAACAACTCTTTTGTGAATATAATTTACTACTTTTGAGAACTCAT
AAAGTAAGCTTTGTAATAAA

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MLNIEDKIFTNLSGSMAVDLASAKKRGDWHATKDLILNSREWIIEEIKKSGLRGRGGAGFPTGLKWSFMPKNSEKATYLV
VNADESEPGTCKDRDIIRYEPHKLIEGCLLAAYAIGAQTCYIYIRGEYYNEALILQNAIQEAYDARLIGLNSCGTDFNFN
LYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPANIGLYGCPTTINNVESIAVVPTILRRGAKWFASIGKPNNTGTK
IFSISGHVNNPCNIEEAMGVPLKYLIEKYAGGVRGGWDNLKAIIPGGSSVPLIPKNICDTVEMDFDSLKSVGSGLGTGGI
IVMDNNTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRIMMRLVKGSASVSDIDMLLDVTKQIEGNTICALGDAAAWP
IQGLIKHFRHEIEDRIKNFNIQ

Sequences:

>Translated_422_residues
MLNIEDKIFTNLSGSMAVDLASAKKRGDWHATKDLILNSREWIIEEIKKSGLRGRGGAGFPTGLKWSFMPKNSEKATYLV
VNADESEPGTCKDRDIIRYEPHKLIEGCLLAAYAIGAQTCYIYIRGEYYNEALILQNAIQEAYDARLIGLNSCGTDFNFN
LYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPANIGLYGCPTTINNVESIAVVPTILRRGAKWFASIGKPNNTGTK
IFSISGHVNNPCNIEEAMGVPLKYLIEKYAGGVRGGWDNLKAIIPGGSSVPLIPKNICDTVEMDFDSLKSVGSGLGTGGI
IVMDNNTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRIMMRLVKGSASVSDIDMLLDVTKQIEGNTICALGDAAAWP
IQGLIKHFRHEIEDRIKNFNIQ
>Mature_422_residues
MLNIEDKIFTNLSGSMAVDLASAKKRGDWHATKDLILNSREWIIEEIKKSGLRGRGGAGFPTGLKWSFMPKNSEKATYLV
VNADESEPGTCKDRDIIRYEPHKLIEGCLLAAYAIGAQTCYIYIRGEYYNEALILQNAIQEAYDARLIGLNSCGTDFNFN
LYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPANIGLYGCPTTINNVESIAVVPTILRRGAKWFASIGKPNNTGTK
IFSISGHVNNPCNIEEAMGVPLKYLIEKYAGGVRGGWDNLKAIIPGGSSVPLIPKNICDTVEMDFDSLKSVGSGLGTGGI
IVMDNNTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRIMMRLVKGSASVSDIDMLLDVTKQIEGNTICALGDAAAWP
IQGLIKHFRHEIEDRIKNFNIQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=419, Percent_Identity=64.6778042959427, Blast_Score=591, Evalue=1e-169,
Organism=Homo sapiens, GI260656005, Length=419, Percent_Identity=64.6778042959427, Blast_Score=590, Evalue=1e-169,
Organism=Escherichia coli, GI1788620, Length=396, Percent_Identity=44.6969696969697, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17531727, Length=422, Percent_Identity=65.4028436018957, Blast_Score=593, Evalue=1e-170,
Organism=Drosophila melanogaster, GI19920794, Length=417, Percent_Identity=65.947242206235, Blast_Score=598, Evalue=1e-171,
Organism=Drosophila melanogaster, GI45550452, Length=413, Percent_Identity=63.680387409201, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24653819, Length=417, Percent_Identity=47.7218225419664, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24653817, Length=417, Percent_Identity=44.1247002398081, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46388; Mature: 46388

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIEDKIFTNLSGSMAVDLASAKKRGDWHATKDLILNSREWIIEEIKKSGLRGRGGAGF
CCCCCHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
PTGLKWSFMPKNSEKATYLVVNADESEPGTCKDRDIIRYEPHKLIEGCLLAAYAIGAQTC
CCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCEEE
YIYIRGEYYNEALILQNAIQEAYDARLIGLNSCGTDFNFNLYLHRGAGAYICGEETALLE
EEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPANIGLYGCPTTINNVESIAVVPTILRRGAKWFASIGKPNNTGTK
HHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCE
IFSISGHVNNPCNIEEAMGVPLKYLIEKYAGGVRGGWDNLKAIIPGGSSVPLIPKNICDT
EEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
VEMDFDSLKSVGSGLGTGGIIVMDNNTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWR
HHCCHHHHHHHHCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IMMRLVKGSASVSDIDMLLDVTKQIEGNTICALGDAAAWPIQGLIKHFRHEIEDRIKNFN
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
IQ
CC
>Mature Secondary Structure
MLNIEDKIFTNLSGSMAVDLASAKKRGDWHATKDLILNSREWIIEEIKKSGLRGRGGAGF
CCCCCHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
PTGLKWSFMPKNSEKATYLVVNADESEPGTCKDRDIIRYEPHKLIEGCLLAAYAIGAQTC
CCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCEEE
YIYIRGEYYNEALILQNAIQEAYDARLIGLNSCGTDFNFNLYLHRGAGAYICGEETALLE
EEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPANIGLYGCPTTINNVESIAVVPTILRRGAKWFASIGKPNNTGTK
HHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCE
IFSISGHVNNPCNIEEAMGVPLKYLIEKYAGGVRGGWDNLKAIIPGGSSVPLIPKNICDT
EEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
VEMDFDSLKSVGSGLGTGGIIVMDNNTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWR
HHCCHHHHHHHHCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IMMRLVKGSASVSDIDMLLDVTKQIEGNTICALGDAAAWPIQGLIKHFRHEIEDRIKNFN
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
IQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA