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The map label for this gene is dnaB [H]

Identifier: 189183795

GI number: 189183795

Start: 930237

End: 931670

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_0888

Alternate gene names: 189183795

Gene position: 931670-930237 (Counterclockwise)

Preceding gene: 189183796

Following gene: 189183792

Centisome position: 46.38

GC content: 32.08

Gene sequence:

>1434_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGGGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTATGAACCATTACATGGTAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGCGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCATTTGAG
GAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACGTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGACCTTGCGCTGCGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCGTATTCTTCTACTTTAG
CAGATTTAGCTATAAGTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTAGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGAC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACTCAACAA
AATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAACTCGAAT
TCTTTCTATAGAATCAGAAATTAACAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGTTGAAGA
CTGTACAAAATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTTGTTGATTATTTACAGCTAATAAAAATTGATAACAGAAG
AAGTCAGTATAATCGAGTACAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGATCTCAATATTTCAA
TCATTGCATTATCTCAATTATCTAGAGCTGTAGAACAAAGGTCAGATAAAAAGCCTCTACTATCAGATCTACGAGAATCA
GGCTCCATTGAACAAGATGCCGATATTGTAATGCTTATATATCGTGACGAATATTACTTGTCTAGATCAGAACCGAATCC
AGGTACTCCAGAATACACAGAATGGGTTACAAAACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACC
GTAATGGGCCAGTTGGTACAGTGAAGTTGCACTATAATAGTAGGTATTCTAAATTTGGCAATATTATAATGTAA

Upstream 100 bases:

>100_bases
AGAGAACTATCTGAAAGATCTTGAGTCCTCTTTTAAAGCTCAAGGATTTTCTTTTGAGTTAGTTAAGTTCGAATAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
CTATAGTTATGCCTTCCGCTATATATAGAACTCATATTTTTCTAGCATACATTCGCATCAAAAGCTTGCTAAGTTTTTGT
AAAAAGGGTTACAAACTATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLRGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIIM

Sequences:

>Translated_477_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLRGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIIM
>Mature_477_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLRGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIIM

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=475, Percent_Identity=35.1578947368421, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53316; Mature: 53316

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLRGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIIM
ECCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCHHHCCCCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLRGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIIM
ECCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA