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The map label for this gene is nusA [H]

Identifier: 189183523

GI number: 189183523

Start: 634253

End: 635755

Strand: Reverse

Name: nusA [H]

Synonym: OTT_0616

Alternate gene names: 189183523

Gene position: 635755-634253 (Counterclockwise)

Preceding gene: 189183524

Following gene: 189183522

Centisome position: 31.65

GC content: 32.0

Gene sequence:

>1503_bases
ATGGTTAATCCTATCAATGCTGAAGTGTTAAGAGTTGTTAATTATATTGCTAGAGATAAGGATATTTCACAAGATGCCTT
AATATCAATTGTAGAGGAGATGATTCAATTGATTAGTAAAAAAATATATGGTGCTGAGCATAACATTAAGGTTGAAATTA
ATAAAAAGAGTGGTGAATGTAAGTTGTATAGAGTGCTTGATGTAGTCGAAGAAACAGAAGATATGTACTCTCAAATCTCT
TTAAATGATGCTTTACAACTTAATGCTAATATTAAGGTTGGAGAACAAATTTTTGAAACTCTTCCTCCTATAAATCTTGG
CAGAGTTTCTGCTCAAACTGCTAGACACTTTATTGCTCAAAAGATTGCAGAAATAGAGAGAAGAAAGCAATATTTAGAAG
TTAAAGATAGAGTCGGAGAAATAATTACTGTAATAGTTTCCAGAATTGATGCAGGTAATATAATAGGAGAGTTTGGTGGA
GTAGAAGTTATTATTCCAAGAGATCAATTAATAAAAAGCGAAGCTTATAAAAAGAACGATAGAGTTAAAGCATATGTACA
AAGAGTAAATGAAGAGCTAAAAGGTCCTCTAATGTTTTTGTCTAGGACTGATAATCAAATGTTGGTTAAGCTATTAGAAA
TGGAGGTTCCAGAAATTTATGAAGGTACAATTATTATTAAAGCAGTAGCAAGAGACCCAGGATCAAGAGCTAAAGTTGCT
GTTTTTTCACCTGATTCATCCATTGATGCTGTTGGATCATGTATAGGTATGAGAGGCAACAGAATTAAAAATATTTCTCA
TGAACTTGCTGGAGAAAGAATTAATGTTATAAAGTGGAGCCAAGATATTGCAGGCTTTGCTATTAATGCTATGTCGCCAG
TACAAATAACGAAAGTAATCATTGATGAGTCTAGACGTTTAATTGAGCTGATAGTATCTGCTAATCAGCTTAGCATTGCT
ATTGGACGACGCGGGCAAAATGTTAGACTTGTATCTAAACTTTTAGGTTGGAATATTGATATTATTACTGAAGAGCAAGA
ATCATCACGTAGAACAGATGAATTCACTTCTGCTACGGATTTATTCACAAAAAATTTAGGAATTGATGAAATGTTGGCTC
AATTATTAGTAGCTGAAGGATTCATCTCGATCGAGCAAATAGCTCATGCTGATCTTAATGCTCTGACTAGTATTGATAAA
CTTAATTCTGAGTTAGTGCTTGAATTGCAACAAAAAGCAATAGATTATGTTAATAATCAAAATAAACTTATTCTTACGAA
ACTAGAGGAGCTAGGAGTAGAGCAAGAGTTATTAGAAACTTTAGAGATTCCATTAGAATCTTTTATAACTCTTGCAGAGA
ATGGTATTAAGAATTTAGAAGATCTTGAAGAACTTACATTTAGCGAGTTTAACACTTTAGTACAGAATAATTGCCTGAGT
GAAGAAGAGTTTAATTATATTATTTTAACTAATAAAAAACGTTTAACTGATTCAGTGGAGTAA

Upstream 100 bases:

>100_bases
TGCTATAGATTATGATAATATAAAGGTTGCAAAGATAGTTTTTACAGATGAAATGTTTAGACAAATCACCAAAAATTATT
AGCTCAGGAGATATATAAAT

Downstream 100 bases:

>100_bases
TATACTCCTGAATAAATATATAAACTTAAGTAGCTAGTTGTTAATTTATTTAATAATAAGTGGTTCACTATAATAAATAT
TGCTGTATAATAAAAAAGCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKIYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMYSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARHFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRVKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTIIIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISHELAGERINVIKWSQDIAGFAINAMSPVQITKVIIDESRRLIELIVSANQLSIA
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGFISIEQIAHADLNALTSIDK
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVQNNCLS
EEEFNYIILTNKKRLTDSVE

Sequences:

>Translated_500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKIYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMYSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARHFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRVKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTIIIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISHELAGERINVIKWSQDIAGFAINAMSPVQITKVIIDESRRLIELIVSANQLSIA
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGFISIEQIAHADLNALTSIDK
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVQNNCLS
EEEFNYIILTNKKRLTDSVE
>Mature_500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKIYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMYSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARHFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRVKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTIIIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISHELAGERINVIKWSQDIAGFAINAMSPVQITKVIIDESRRLIELIVSANQLSIA
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGFISIEQIAHADLNALTSIDK
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVQNNCLS
EEEFNYIILTNKKRLTDSVE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=498, Percent_Identity=34.3373493975904, Blast_Score=295, Evalue=4e-81,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56307; Mature: 56307

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKIYGAEHNIKVEINKKSGEC
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
KLYRVLDVVEETEDMYSQISLNDALQLNANIKVGEQIFETLPPINLGRVSAQTARHFIAQ
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGGVEVIIPRDQLIKSEAYKKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEECHHHHHHHHHHCCCH
RVKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTIIIKAVARDPGSRAKVA
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHCCEEEEEEECCCCCCCCEEE
VFSPDSSIDAVGSCIGMRGNRIKNISHELAGERINVIKWSQDIAGFAINAMSPVQITKVI
EECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCHHHHHHHH
IDESRRLIELIVSANQLSIAIGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATD
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
LFTKNLGIDEMLAQLLVAEGFISIEQIAHADLNALTSIDKLNSELVLELQQKAIDYVNNQ
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
NKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVQNNCLS
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
EEEFNYIILTNKKRLTDSVE
CCCCCEEEEECCHHCCCCCC
>Mature Secondary Structure
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKIYGAEHNIKVEINKKSGEC
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
KLYRVLDVVEETEDMYSQISLNDALQLNANIKVGEQIFETLPPINLGRVSAQTARHFIAQ
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGGVEVIIPRDQLIKSEAYKKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEECHHHHHHHHHHCCCH
RVKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTIIIKAVARDPGSRAKVA
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHCCEEEEEEECCCCCCCCEEE
VFSPDSSIDAVGSCIGMRGNRIKNISHELAGERINVIKWSQDIAGFAINAMSPVQITKVI
EECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCHHHHHHHH
IDESRRLIELIVSANQLSIAIGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATD
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
LFTKNLGIDEMLAQLLVAEGFISIEQIAHADLNALTSIDKLNSELVLELQQKAIDYVNNQ
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
NKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVQNNCLS
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
EEEFNYIILTNKKRLTDSVE
CCCCCEEEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]