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The map label for this gene is dnaB [H]

Identifier: 189183470

GI number: 189183470

Start: 576930

End: 577952

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_0563

Alternate gene names: 189183470

Gene position: 577952-576930 (Counterclockwise)

Preceding gene: 189183471

Following gene: 189183467

Centisome position: 28.77

GC content: 31.28

Gene sequence:

>1023_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAATAATATTCAGGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTACGAGCCATTACATGGTAAAATATATAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCATTTGAG
GAAATAGGTGGAGTAGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGCGGCGTTATTTAATTGAAATTGGAAAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGAC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACTCAACAA
AATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAACTCGAAT
TCTTTCTATAGAATCAGAAATTAACAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGTTGAAGA
CTGTACAAGATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCAATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTAAAAACTCTCAGCTGCCCTTAG

Upstream 100 bases:

>100_bases
GAGAGAACTATCTGAAAGATCTTGAGCCCGCTTTTAAAGCTCAAGGATTTTCTTTCGAGCTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
ATCGTACTAGATACTGCTTCCTATTAATTTCTTTGTTTATATAATTGCTTCTCTTGCAACTTATTCTATCTCCAAACTTA
ATTCCTATCTTATTCTCTCT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILLKTLSCP

Sequences:

>Translated_340_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILLKTLSCP
>Mature_340_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILLKTLSCP

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=328, Percent_Identity=29.2682926829268, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37361; Mature: 37361

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILLKTLSCP
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
>Mature Secondary Structure
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILLKTLSCP
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA