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The map label for this gene is gidA [H]

Identifier: 189183418

GI number: 189183418

Start: 532310

End: 534178

Strand: Reverse

Name: gidA [H]

Synonym: OTT_0511

Alternate gene names: 189183418

Gene position: 534178-532310 (Counterclockwise)

Preceding gene: 189183419

Following gene: 189183411

Centisome position: 26.59

GC content: 34.08

Gene sequence:

>1869_bases
ATGAATAAATTTGATGTTGTAGTAGTTGGTGGTGGCCATGCTGGCTGTGAAGCAGCAGCAGCAGCAGCGCGAATTGGAGT
TAAAGTTGCATTAATTACTCTTAAACCTGAAAATCTTGGAGAAATGCCATGTAACCCAGCTATTGGTGGTCGTGGTAAGG
GGCATATAGTCAAAGAAGTTGATGCTCTTGATGGCCTTATTGGGTACATAGCAGATCAAGCTGGCATACACTATAAAATG
TTGAATCATACTAAAGGACCAGCTGTTTGGGGACCTAGGGCACAAATAGATCGTGCGCTATATAAATCTGCAATGTACAG
TACAATAATGAATTATCCTAATTTAACTACAATATTTGCATCAGTAGAGGATATTAAAGTTATTGCTAATAAAGTTACTG
CAGTAATTGTTAATGGAAAAGAAATTTATTGTCAAAAGGTTATACTAACTACTGGTACTTTCTTATCTGGCGTAATTCAC
CGTGGTAAAGAGCAAATAAAAGCTGGACGCTTGGGAGAAAATGCATCTTATGGATTATCTAATACTTTATCAGAACTTGG
ATTGAGACTAGGTAGATTAAAAACTGGCACTCCGCCCAGAATTGATAGTAGAACCATAGATTACTCAAAATTAGAGGAGC
AACCAGGGGATTTAATTCCTACACCATTTTCTGAAATTACTAAGAAGGTCTTAGTTCCGCAAATTAAGTGTTATATTACT
CGAACTAATGAAATAACACATAAAATTATAAAGGAAAATTTACATCTTTCTGCAATGTATAGTGGCCAAATTCAGGGAAC
AGGCCCAAGATATTGTCCATCAATAGAGGATAAAATTATAAGGTTTAGTCATAATGCGAGTCATCAAATCTTTTTAGAGC
CAGAAGGATTAAACAGTAACACTATCTATCCTAATGGAATATCAACATCTTTACCAAGTGATGTGCAAGAAAATATGATT
AGAACTATAGAAGGTTTGGAAAATTGCAAAATAATAGCATATGGCTACGCTATTGAATATGATTATGTTGATCCTAGACA
ACTAAAGCGTAGCCTAGAAGTGAAATCTGTAGATGGGCTATATTTAGCTGGACAGATTAATGGTACTACAGGTTATGAGG
AAGCAGCAGGGCAAGGAATCATGGCAGGAATAAATGCTGCACTAGCAGTAAAAAAGCAAGATCCATTTATCTTAGATAGA
ACAGATGCTTATATTGGAGTAATGATTGATGATTTAACAAACGGAATAGATGAACCTTACCGTATGTTTACTTCAAGATC
AGAATACAGATTAAGTATTAGAGCTGACAATGCTGATCAGCGTCTCACTCCCAAAGCAATAGATCTAGGTTGTGTAAGTC
AGTTAAGGAAAGAGGTATTTCAGAAAAAATTATCTAAATTAAATACTTTACGTGATTATGTTCAGTCACTAACCATTACT
CCTAAACAACTTCAAAATTGCGGTTATCAAATTTCACAGAATGGTATAGCACGTACTGCGTTTAGCTTACTTGGACTTCC
AAATTTTGGTATTAATATAGTAAAAGATATTTACCCTGAATTAAATCGATATGATAACAATTTATTATTATTACTAACTT
ATGAATCTAAATATCATGCATATTTAGAGCGCCAAAAAGAAGACATTGTGCTTTTTAAGCAAGAAGAAACATATCAAATT
CCTCAAGATTTGAACTATGACCAGATTCCTAGTTTATCAATTGAAGTACGAGAAAAGCTTAAGCAGTCTCAACCTGAGAC
TATAAGAGCTGCAAAACATATAAATGGAGTAACTCCATCAGCAATTATGGCAATAATCATCTTTTTAAAAACTAAATATT
TGAATTCTTCCATAACTGATAAAAAATGA

Upstream 100 bases:

>100_bases
TACATAATGTTATTAGTTTAACTACCGCATGTTAGGTAGTTGATTGTTATATAATCTATAATAAGCTAGCTTAGTTAGTT
TTTAGTAAAATGTTGAAAGA

Downstream 100 bases:

>100_bases
TTATATGTTATCTAACTTTTGAATATTAGATAAGTACATAGCAATTTTAACACTAAATTAATTAATACAAGAGAACCAAC
ATATAAACTAAAAATTAATA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKRSLEVKSVDGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRKEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHAYLERQKEDIVLFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQSQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK

Sequences:

>Translated_622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKRSLEVKSVDGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRKEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHAYLERQKEDIVLFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQSQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK
>Mature_622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKRSLEVKSVDGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRKEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHAYLERQKEDIVLFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQSQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=47.7777777777778, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=45.9541984732824, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=44.9253731343284, Blast_Score=510, Evalue=1e-144,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=48.6356340288925, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=41.3008130081301, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=46.8051118210863, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=45.9587955625991, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69074; Mature: 69074

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEV
CCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DALDGLIGYIADQAGIHYKMLNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHH
SVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIHRGKEQIKAGRLGENASYGLS
HHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
NTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEE
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSN
ECHHHHHHHHHCCCEEEEEEECEECCCCCCCCCCHHHHHEEEECCCCCEEEEECCCCCCC
TIYPNGISTSLPSDVQENMIRTIEGLENCKIIAYGYAIEYDYVDPRQLKRSLEVKSVDGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCE
YLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDRTDAYIGVMIDDLTNGIDEPY
EEEEEECCCCCCHHHCCCCEEECCCEEEEEECCCCEEEECCCCEEEEEEHHHHCCCCHHH
RMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRKEVFQKKLSKLNTLRDYVQSLTIT
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHA
HHHHHHCCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
YLERQKEDIVLFKQEETYQIPQDLNYDQIPSLSIEVREKLKQSQPETIRAAKHINGVTPS
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHH
AIMAIIIFLKTKYLNSSITDKK
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEV
CCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DALDGLIGYIADQAGIHYKMLNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHH
SVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIHRGKEQIKAGRLGENASYGLS
HHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
NTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEE
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSN
ECHHHHHHHHHCCCEEEEEEECEECCCCCCCCCCHHHHHEEEECCCCCEEEEECCCCCCC
TIYPNGISTSLPSDVQENMIRTIEGLENCKIIAYGYAIEYDYVDPRQLKRSLEVKSVDGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCE
YLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDRTDAYIGVMIDDLTNGIDEPY
EEEEEECCCCCCHHHCCCCEEECCCEEEEEECCCCEEEECCCCEEEEEEHHHHCCCCHHH
RMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRKEVFQKKLSKLNTLRDYVQSLTIT
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHA
HHHHHHCCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
YLERQKEDIVLFKQEETYQIPQDLNYDQIPSLSIEVREKLKQSQPETIRAAKHINGVTPS
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHH
AIMAIIIFLKTKYLNSSITDKK
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA