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The map label for this gene is prfB [C]

Identifier: 189183342

GI number: 189183342

Start: 454471

End: 454731

Strand: Reverse

Name: prfB [C]

Synonym: OTT_0435

Alternate gene names: 189183342

Gene position: 454731-454471 (Counterclockwise)

Preceding gene: 189183343

Following gene: 189183340

Centisome position: 22.63

GC content: 28.74

Gene sequence:

>261_bases
ATGTTTCCAAAAATCAATATTTTTATAGACGTTAAGGACATTGTTGTTGATATTAACCCAGTAGATTATGCCGTTAAAAT
TACACATATTCCTACTAGTATTATAACGGAATGTAGAAGTTATAAATCACACTACAAAAACAAGATAGAAGCTATATTAT
CCTTAAAAAAAACACTTTATGAAAGAGAACTAAAAAAAAATAGCTCTATGAAAACTACCTTGCCTAGCCGCTCAGTATTA
GGCTATAATGCATTGCAATAA

Upstream 100 bases:

>100_bases
TTGTAGTATACTGCTTATGATGAGTTGAGATATAATCTCATCATTAGTAAATACAAAAATGAAATAGATTTTTCAAAAAT
TTAAGATAGAAACTTCATAT

Downstream 100 bases:

>100_bases
AGGGAGATTGTTTTAATGACGATTTAACCAAATTAGAACAAATGAGCTTTAAGATGCTTTTAAGACTATTACAGAAGAAT
TTTTGTTTTTTAGCTTTTGT

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 86; Mature: 86

Protein sequence:

>86_residues
MFPKINIFIDVKDIVVDINPVDYAVKITHIPTSIITECRSYKSHYKNKIEAILSLKKTLYERELKKNSSMKTTLPSRSVL
GYNALQ

Sequences:

>Translated_86_residues
MFPKINIFIDVKDIVVDINPVDYAVKITHIPTSIITECRSYKSHYKNKIEAILSLKKTLYERELKKNSSMKTTLPSRSVL
GYNALQ
>Mature_86_residues
MFPKINIFIDVKDIVVDINPVDYAVKITHIPTSIITECRSYKSHYKNKIEAILSLKKTLYERELKKNSSMKTTLPSRSVL
GYNALQ

Specific function: Peptide Chain Release Factor 2 Directs The Termination Of Translation In Response To The Peptide Chain Termination Codons Uga And Uaa. [C]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI2367172, Length=100, Percent_Identity=32, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 9916; Mature: 9916

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPKINIFIDVKDIVVDINPVDYAVKITHIPTSIITECRSYKSHYKNKIEAILSLKKTLY
CCCCEEEEEEEHHEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELKKNSSMKTTLPSRSVLGYNALQ
HHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPKINIFIDVKDIVVDINPVDYAVKITHIPTSIITECRSYKSHYKNKIEAILSLKKTLY
CCCCEEEEEEEHHEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELKKNSSMKTTLPSRSVLGYNALQ
HHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA