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The map label for this gene is traG [H]

Identifier: 189183310

GI number: 189183310

Start: 423750

End: 426077

Strand: Reverse

Name: traG [H]

Synonym: OTT_0403

Alternate gene names: 189183310

Gene position: 426077-423750 (Counterclockwise)

Preceding gene: 189183311

Following gene: 189183309

Centisome position: 21.21

GC content: 34.75

Gene sequence:

>2328_bases
ATGTTTGCTTCTCTTTCCTCGCAAACGAGTTATTTTGTATCTAAAATGTTGGAAAATCATCTTTTACCGGCTTATGAAGG
ACTATCTAGCAGAAAAACTGGCATTATGTTTGGAGCTAAGGCTGTAGCTAAGATTCGAGATGTGCAAATACATGATCCAG
TAACTTTAACTAATACTAAGGAATTTCTTAGACAATGCTTTATGAAGCCTTACATCATAGGTAATATCTTAGGTAAAAAA
GCAGCTGCTCAACAAACTAATGATATCATAGGATTTATCGAGCAGAATATACCTAATAATTTCGGTATTTATTATCGCGA
ACCTAGTAATTTAGGTATTAGTTTCAAGACATGCAGACAAGCTACTCCATTAATTAAGGCAGCAATTCATAAAGAATTAA
ACGAAGGACTTCTAACCAAGTTTGCAGCTGCGATTGGAGTTCAATCTGATCAGTCACACATGTTAAGTCAAAGACTAAAA
GTCATGACTGGAGATACACTAAAATATCTACAAAGAGAACAACAAGACATTCATGAATGGATGAAGCAAGCTATGCTACT
TAATGCTAATCGTGAGTCATATGATGACTGGCGTGAGAAGTTTTCATTATCACGAATTTATCCTAATCTGGTGAGTATGC
ATGCAATCAGAGGGCTCTTTCAAAAATCATTTTCGTATCTAGTCGCTGGGGAAATGGCTGCTCACATGATGCCTATTTTA
CAATCTGTTTTTTTTGCATTAGTAGTCTCAATGATCTTTATTGTATTTCCAATGGGCCTATTACCTGGCGGCTATAACAT
ACTCAAAACATGGATTTTGTTAATAATATGGGTCAGCAGCTGGCCAGTATTTTTTACAATAATCCATTGTCTAGGAATGA
TCAGCTTATCAAGCAAATCTGGAGCTTTCGGTAGTGATTATGGGCTGAATATGCTATCGCAAGGAAGCTTTGCAGAAATG
ATTTTATATAGCTATGCAACGTTTCAAATGCTTGCATCATCAATACCAATGCTTTCTTGGGCAGTGATAAAAGCTTGCGC
TCATGCTACAGCTAACTTAGCTAGCCAGTTCTCTCCTATACAAGTTGCTAGCAGCTTAGGAAGTAATATAGTCGACAATA
ACTTGAGTATGGATAATTACAGCATAGGCAATAGAACTATTTCTCAACAAAACCTAGCTCCTTCATTAGATATGACTGCT
AGTATTATCAATTATGGTAGCTACAAAATTATTACTGGAAATAATGGCGAGCAATTTGTAATTCAAACTGTAGATTCATT
GGGAAATAACTTCCGCTCTTCTGCACTGCTACAAACTGGATATCAAAACCAGTTTGTAAGTTCTCAGAGCATGCTTAATT
CACTGACACAAAAAGAGGCTAATCTTATTTCAACAGGAAACTCCATGGCCATAGACATATGCAGAAGGTTAACTCATGAT
GAAGCTCTGTCTATAGGCCTAACTGAGAGTGAATACCAAGCTCTGCAAAAGGTAAGTTCTGATAGTGAGTCTTTTAGTCA
GCATACAGGTAGTAGCCACAGTAAAAGTAGTGGAACTAGTGCTAGTGCTGGTGGTGGATCGTTTTTTGCTAAAGTTGAAG
TTTCAGGAGGACGAAGACATAATAATGAACAAGGCAACTCAGTAAATCAGCAACAGTCATATAATGAAGCAGTATCCAAA
ATTCAAAGCGCAGTTCAAGAAGGAAAATTTAGTAGTACAAATAGCGAAGTACAGTCACTAAGTGAAAATCTAAATGCTAA
TCTTTCTGAGCAACAATCTATTGGACAAGAAATAGCTAAAACTAAACAAGAAATGGAGCAACTTAGTTATAGCATGAATT
ATGTATCTCAAAACTCTATCACTATTGATCGTAACATCAATGAACTTGTATTAAATGAAATTATTGCTCAAAATCCTGAA
ATAAGAAGCAAGGAGCAAGCAGCTAGATGGATAATAAACCACTCTGCTGAAGCAGAAAAAATTGCTTTTGAGGTTGCTAA
AATCAACAATGAAGTACCTAAAAATTTGAATGATCATATTAATGATGGTAACTTTTCGACTAAAAAAGATCTACAATCAG
ATTTTTACAAAAATGTTGAGCAATTAGAAGCTATCTATGATAAAAGCAATCAAACGCATTCTCAAAATATAGAACAAGCT
TTTGTTGATAATGAAAAAGTAGAGAATCTTGATCAAATTTCTGTTAATATAAAAAATAAGGCTCAAGAAATCCAGAAGGA
ATTTGATAATACCTCTAATTCAGCAGTTATTTTGACAGGTAAAGAGGTATTAAAAGCAGTAAAAAATTTAAATGGTACTA
AAGATTAG

Upstream 100 bases:

>100_bases
TTAATCAATCAGAAATAATAATTTTATAAAAACTTACATCTTTTTTATAAAATTATTATTTCTTTTCTTGATTCTTATCA
CGGTATCTATAGGTATTGCA

Downstream 100 bases:

>100_bases
TAATTAATATGAAAAACGTGTGTAAAAATTTAGCCATTATTTTATCATTAATATTATTAAATACACCAGCTGTTGCAGCA
GAGCAAAGCATACAACAAAA

Product: putative conjugative transfer protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWAVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSMDNYSIGNRTISQQNLAPSLDMTA
SIINYGSYKIITGNNGEQFVIQTVDSLGNNFRSSALLQTGYQNQFVSSQSMLNSLTQKEANLISTGNSMAIDICRRLTHD
EALSIGLTESEYQALQKVSSDSESFSQHTGSSHSKSSGTSASAGGGSFFAKVEVSGGRRHNNEQGNSVNQQQSYNEAVSK
IQSAVQEGKFSSTNSEVQSLSENLNANLSEQQSIGQEIAKTKQEMEQLSYSMNYVSQNSITIDRNINELVLNEIIAQNPE
IRSKEQAARWIINHSAEAEKIAFEVAKINNEVPKNLNDHINDGNFSTKKDLQSDFYKNVEQLEAIYDKSNQTHSQNIEQA
FVDNEKVENLDQISVNIKNKAQEIQKEFDNTSNSAVILTGKEVLKAVKNLNGTKD

Sequences:

>Translated_775_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWAVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSMDNYSIGNRTISQQNLAPSLDMTA
SIINYGSYKIITGNNGEQFVIQTVDSLGNNFRSSALLQTGYQNQFVSSQSMLNSLTQKEANLISTGNSMAIDICRRLTHD
EALSIGLTESEYQALQKVSSDSESFSQHTGSSHSKSSGTSASAGGGSFFAKVEVSGGRRHNNEQGNSVNQQQSYNEAVSK
IQSAVQEGKFSSTNSEVQSLSENLNANLSEQQSIGQEIAKTKQEMEQLSYSMNYVSQNSITIDRNINELVLNEIIAQNPE
IRSKEQAARWIINHSAEAEKIAFEVAKINNEVPKNLNDHINDGNFSTKKDLQSDFYKNVEQLEAIYDKSNQTHSQNIEQA
FVDNEKVENLDQISVNIKNKAQEIQKEFDNTSNSAVILTGKEVLKAVKNLNGTKD
>Mature_775_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWAVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSMDNYSIGNRTISQQNLAPSLDMTA
SIINYGSYKIITGNNGEQFVIQTVDSLGNNFRSSALLQTGYQNQFVSSQSMLNSLTQKEANLISTGNSMAIDICRRLTHD
EALSIGLTESEYQALQKVSSDSESFSQHTGSSHSKSSGTSASAGGGSFFAKVEVSGGRRHNNEQGNSVNQQQSYNEAVSK
IQSAVQEGKFSSTNSEVQSLSENLNANLSEQQSIGQEIAKTKQEMEQLSYSMNYVSQNSITIDRNINELVLNEIIAQNPE
IRSKEQAARWIINHSAEAEKIAFEVAKINNEVPKNLNDHINDGNFSTKKDLQSDFYKNVEQLEAIYDKSNQTHSQNIEQA
FVDNEKVENLDQISVNIKNKAQEIQKEFDNTSNSAVILTGKEVLKAVKNLNGTKD

Specific function: Plays a crucial role in donor-recipient cell interactions. Required for two stages of the conjugation process:pilus biosynthesis and mating aggregate stabilization. May interact with traN [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012931 [H]

Pfam domain/function: PF07916 TraG_N [H]

EC number: NA

Molecular weight: Translated: 86029; Mature: 86029

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTK
CCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHCEEECCCEEECCHH
EFLRQCFMKPYIIGNILGKKAAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHH
ATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLKVMTGDTLKYLQREQQDIHEW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
GAFGSDYGLNMLSQGSFAEMILYSYATFQMLASSIPMLSWAVIKACAHATANLASQFSPI
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
QVASSLGSNIVDNNLSMDNYSIGNRTISQQNLAPSLDMTASIINYGSYKIITGNNGEQFV
HHHHHHCCHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEEECCCCCEEH
IQTVDSLGNNFRSSALLQTGYQNQFVSSQSMLNSLTQKEANLISTGNSMAIDICRRLTHD
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
EALSIGLTESEYQALQKVSSDSESFSQHTGSSHSKSSGTSASAGGGSFFAKVEVSGGRRH
HHHEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NNEQGNSVNQQQSYNEAVSKIQSAVQEGKFSSTNSEVQSLSENLNANLSEQQSIGQEIAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TKQEMEQLSYSMNYVSQNSITIDRNINELVLNEIIAQNPEIRSKEQAARWIINHSAEAEK
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHH
IAFEVAKINNEVPKNLNDHINDGNFSTKKDLQSDFYKNVEQLEAIYDKSNQTHSQNIEQA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVDNEKVENLDQISVNIKNKAQEIQKEFDNTSNSAVILTGKEVLKAVKNLNGTKD
HCCCHHHCCHHHHEEEHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTK
CCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHCEEECCCEEECCHH
EFLRQCFMKPYIIGNILGKKAAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHH
ATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLKVMTGDTLKYLQREQQDIHEW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
GAFGSDYGLNMLSQGSFAEMILYSYATFQMLASSIPMLSWAVIKACAHATANLASQFSPI
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
QVASSLGSNIVDNNLSMDNYSIGNRTISQQNLAPSLDMTASIINYGSYKIITGNNGEQFV
HHHHHHCCHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEEECCCCCEEH
IQTVDSLGNNFRSSALLQTGYQNQFVSSQSMLNSLTQKEANLISTGNSMAIDICRRLTHD
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
EALSIGLTESEYQALQKVSSDSESFSQHTGSSHSKSSGTSASAGGGSFFAKVEVSGGRRH
HHHEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NNEQGNSVNQQQSYNEAVSKIQSAVQEGKFSSTNSEVQSLSENLNANLSEQQSIGQEIAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TKQEMEQLSYSMNYVSQNSITIDRNINELVLNEIIAQNPEIRSKEQAARWIINHSAEAEK
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHH
IAFEVAKINNEVPKNLNDHINDGNFSTKKDLQSDFYKNVEQLEAIYDKSNQTHSQNIEQA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVDNEKVENLDQISVNIKNKAQEIQKEFDNTSNSAVILTGKEVLKAVKNLNGTKD
HCCCHHHCCHHHHEEEHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1348105; 7915817 [H]