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The map label for this gene is gatB [H]

Identifier: 189183273

GI number: 189183273

Start: 372386

End: 373846

Strand: Reverse

Name: gatB [H]

Synonym: OTT_0366

Alternate gene names: 189183273

Gene position: 373846-372386 (Counterclockwise)

Preceding gene: 189183274

Following gene: 189183268

Centisome position: 18.61

GC content: 30.87

Gene sequence:

>1461_bases
ATGACATTTATAGAAGGCAAAACTGAAAAATGGGAATATGTTATAGGACTTGAAATTCATGCCCAAATAAAATCCAATGC
TAAGTTATTTTCTAGCGCTTCAACTGAATTTGGAAGTTCTCCAAACTCACAGGTCGAATTGTTAGATGCAGCTATGCCAG
GGTCTTTACCAGTATTAAATGAATTTTGTGTGCATCAAGCTATAAAAACTGCTCTAGGAATTAATGCGAAAATAAATAAA
TTATCTATTTTTGACCGTAAAAACTACTTTTATGCTGATTTACCAGCAGGTTATCAAATTTCGCAATTTTATCATCCAAT
TGCTCAAGGCGGTTGGATAGAAATATTAGATGAAAATGGTAATATTAAGCGCATTCAGATTAATAGGTTGCATATAGAAC
AAGATACAGGAAAAAGTACCCACGATCAATCGGACACTTATAGCTATATAGATCTTAATCGAAGTGGTATAGCATTAATG
GAGATAGTTTCTGAACCTGATATTTCTTCTCCTATGCAAGCAGCCGAATATATCAAAAAACTTAGAGCAATATTGAGATA
TTTAGATAGTTGTAATGGTGATATGGAAAAAGGGTCTCTAAGATGCGATGCTAACATATCAGTAAGAAAGCCAAATAGTG
AATTAGGAACTAAATGTGAAATTAAGAACCTTAATTCAATTAAATCAATTGTTAGAGCACTAGAATTTGAAGGTCAAAGG
CAGGTCAATATATTAGAATCTGGTGGTACAGTTAAACAGGAAAGTCTTCTATTTGATGCTACACTAGGCAAAACATTTCC
AATGAGATCTAAAGAGAATGCTACCGATTATAGATATTTTCCGGATCCAGACCTACCTCCAATAATATTAGATCAGTCAT
TAATTGACAATATTGCTAGTAGTTTACCTGAATTACCAGATGCCAAAATTAGAAGATATATTAATGAAATCAAACTTAGT
GATTATAATGCTCAAGTTTTAGCAGCTGATAAGGATATTTCATGCTTTTTTGAAGAAGTTATTAAAACTGCAAATCCGCT
ATTAAGTGCTAACTGGATATTAAGTGAGTTATTTGGATTAATGAATAAAGATGGTATTACTATTAACGAATGTAAAATTA
CTGCTAATCACTTTTCTGAATTAATACAGTTGATTAGTTCCAAAGCAATATCTAGCAAAATTGCAAAAACAGTCTTAAAA
GAAATGTTTGATAGTGGAAAAAGTCCAAAGATAATTATGCAAGAAAAAAATATACAGCAAATTTCAGATCCTAATCAAAT
TGCTGATATTATTGATGATGTACTTAAGGATAATTATCAGTCTGTTGTATCCTATAGAAATGGTAAAGACAGATTATTTG
GATTTTTTGTTGGCCAAGTAATGAAAAAAACTGCTGGTAATGCAAATCCTGCACTTATCAATGAAATATTGCATACAAAA
TTAAAACAATTTCAAATTTAA

Upstream 100 bases:

>100_bases
AAGTTATTGGTTCTCGCTTAGATGAGTATAATATTATTAAAATATCATCAGCTATTGAAAAAGCACTCAATCTCAAATTT
ATTCCAAAAGGTTTTTAAAT

Downstream 100 bases:

>100_bases
ATTTGTTTTCTAATCTAATAAAAGTCTAACATCAATAAAACAGTTTATTTGTTTTGATTTTTTATACAATCTCTTATCCA
TATATTCAGCAATTACCTCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINK
LSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKRIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALM
EIVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGSLRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKIRRYINEIKLS
DYNAQVLAADKDISCFFEEVIKTANPLLSANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLK
EMFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPALINEILHTK
LKQFQI

Sequences:

>Translated_486_residues
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINK
LSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKRIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALM
EIVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGSLRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKIRRYINEIKLS
DYNAQVLAADKDISCFFEEVIKTANPLLSANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLK
EMFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPALINEILHTK
LKQFQI
>Mature_485_residues
TFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINKL
SIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKRIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALME
IVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGSLRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQRQ
VNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKIRRYINEIKLSD
YNAQVLAADKDISCFFEEVIKTANPLLSANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKE
MFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPALINEILHTKL
KQFQI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=39.4789579158317, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=466, Percent_Identity=32.8326180257511, Blast_Score=230, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=519, Percent_Identity=32.3699421965318, Blast_Score=249, Evalue=5e-67,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=33.9430894308943, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54495; Mature: 54364

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLN
CCCCCCCCCCEEEEEEEEEEEEHHCCHHHHHCCCHHCCCCCCCCEEEEECCCCCCCHHHH
EFCVHQAIKTALGINAKINKLSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENG
HHHHHHHHHHHHCCCCEECEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
NIKRIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALMEIVSEPDISSPMQAAEYIKK
CEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHH
LRAILRYLDSCNGDMEKGSLRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
HHHHHHHHHHCCCCCCCCCEEECCCEEEECCCHHHCCEEEECCHHHHHHHHHHHHCCCCC
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIAS
EEEEEECCCCEEHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHH
SLPELPDAKIRRYINEIKLSDYNAQVLAADKDISCFFEEVIKTANPLLSANWILSELFGL
HCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKEMFDSGKSPKIIMQEKNIQQ
HCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
ISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPALINEILHTK
CCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKQFQI
HHHCCC
>Mature Secondary Structure 
TFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLN
CCCCCCCCCEEEEEEEEEEEEHHCCHHHHHCCCHHCCCCCCCCEEEEECCCCCCCHHHH
EFCVHQAIKTALGINAKINKLSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENG
HHHHHHHHHHHHCCCCEECEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
NIKRIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALMEIVSEPDISSPMQAAEYIKK
CEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHH
LRAILRYLDSCNGDMEKGSLRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
HHHHHHHHHHCCCCCCCCCEEECCCEEEECCCHHHCCEEEECCHHHHHHHHHHHHCCCCC
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIAS
EEEEEECCCCEEHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHH
SLPELPDAKIRRYINEIKLSDYNAQVLAADKDISCFFEEVIKTANPLLSANWILSELFGL
HCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKEMFDSGKSPKIIMQEKNIQQ
HCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
ISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPALINEILHTK
CCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKQFQI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA