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The map label for this gene is gatA [H]

Identifier: 189183274

GI number: 189183274

Start: 373849

End: 375327

Strand: Reverse

Name: gatA [H]

Synonym: OTT_0367

Alternate gene names: 189183274

Gene position: 375327-373849 (Counterclockwise)

Preceding gene: 189183275

Following gene: 189183273

Centisome position: 18.68

GC content: 33.81

Gene sequence:

>1479_bases
ATGAACAATCTAGTAAAATTAACAATTTCTCAAGCAATTAGCGGGCTAAAAAATAAAGAGTTTACTGCTACTGATCTAGT
AACTGCACATATCAACCAAATGGATAAGTATAGGTGTATTAATGCTTATATTACTGAGATACCAGAACTTGCTTTACAGG
CAGCTAAAAAATCTGATTTTTTAATTCAAACTAATCAAGCTCGGCCAATTGAAGGTATTCCGGTTTCTATTAAGGATCTT
TTCTGTACTAAAGGAATATTAACTACTGCTGCATCAAAGATGCTACAAAACTTTACTCCTGTTTATGATGCTACTGTATA
TAGTAAAATCATTAATGCTGGCGGCATTATGCTAGGAAAAGGTAATATGGATGAGTTTGCTATGGGATCAGCAAACATTA
ATAGTTATTTTGGCAATGTAATTAATCCCTGGAAGGCTGAAAATGATGATATAGATTTAACTCCTGGTGGATCTTCAGGT
GGATCTTCAGCTGCCGTTGCAGCATTTATGGCAATGGCAGCATTAGGTAGTGATACTGGAGGATCAGTTAGGCAACCAGC
GTCTTATACTGGAACTGTTGGTATAAAGCCGAGTTATGGACGATGCTCAAGATGGGGTATGATTGCATTTTCATGCTCGC
TAGATCAAGCTGGAATAATTACTCGCACAGTTGAAGATGCAGCAATTATGCTAGAAACCATGATGGGCTATGATGAAAAG
GATTCAACAAGCTTAAATGTAGAAGTTCCTAACCTAAGATCAGCTGTTAATCAACCTATTAAAGGTATGAAAATAGGTAT
ACCTTATGATTTAATGGAAAATAAGAGCTTAAGTAGTGAAATTATCACAATGTGGCAAAACACTATAAATTTGTTAAAAG
ATCATGGTGTAGAAATTGTATCTATTAGCTTGCCATATATTAATTATGCATTACCAGTATATTATGTTCTGTCTTCTGCT
GAAGCTTCATCAAATTTAGCAAGATATGATGGAGTAAGATATGGGTTAAGAGTAGAGGGAAAAAACTCAAATATTAATGA
AATTTATGAACTTTCCAGATCAGAAGGATTTGGAGCTGAAGTTAAAAGAAGAATTATGATGGGTACATATGCACTTGCAT
CAACAAATATCAATTCGTATTATATTAAAGCTCAACAAGTAAGACGATTAATAGTGAATGACTTCACAAATAGTTTTAAT
AAAGTAGATTGTATATTAATTCCATCTGCGCCTACTGCAGCTTTTCCACTTAACTCTAATCAAGATGATCCAGTTACTAT
GTATTTAAATGATATATTGACAATACCAGCAAGTTTAGCTGGGTTGCCATGCATTTCAATACCAGCTGGGTTTTCAGCTA
ACAAGTTGCCATTAGGTATGCAAGTTATTGGTTCTCGCTTAGATGAGTATAATATTATTAAAATATCATCAGCTATTGAA
AAAGCACTCAATCTCAAATTTATTCCAAAAGGTTTTTAA

Upstream 100 bases:

>100_bases
AGAATATTACAGTGGATCAGTTATTATCTAACGTACCACAGTCATCAGCAACTATAGCTAAAAACACAAAATATTTTGTT
GTACCAAAGATAATAGAATA

Downstream 100 bases:

>100_bases
ATATGACATTTATAGAAGGCAAAACTGAAAAATGGGAATATGTTATAGGACTTGAAATTCATGCCCAAATAAAATCCAAT
GCTAAGTTATTTTCTAGCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNNLVKLTISQAISGLKNKEFTATDLVTAHINQMDKYRCINAYITEIPELALQAAKKSDFLIQTNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATVYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAENDDIDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQAGIITRTVEDAAIMLETMMGYDEK
DSTSLNVEVPNLRSAVNQPIKGMKIGIPYDLMENKSLSSEIITMWQNTINLLKDHGVEIVSISLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKNSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF

Sequences:

>Translated_492_residues
MNNLVKLTISQAISGLKNKEFTATDLVTAHINQMDKYRCINAYITEIPELALQAAKKSDFLIQTNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATVYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAENDDIDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQAGIITRTVEDAAIMLETMMGYDEK
DSTSLNVEVPNLRSAVNQPIKGMKIGIPYDLMENKSLSSEIITMWQNTINLLKDHGVEIVSISLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKNSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF
>Mature_492_residues
MNNLVKLTISQAISGLKNKEFTATDLVTAHINQMDKYRCINAYITEIPELALQAAKKSDFLIQTNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATVYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAENDDIDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQAGIITRTVEDAAIMLETMMGYDEK
DSTSLNVEVPNLRSAVNQPIKGMKIGIPYDLMENKSLSSEIITMWQNTINLLKDHGVEIVSISLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKNSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=38.7795275590551, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI195972892, Length=516, Percent_Identity=24.031007751938, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=37.855579868709, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17537465, Length=516, Percent_Identity=24.4186046511628, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=241, Percent_Identity=29.045643153527, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=344, Percent_Identity=24.4186046511628, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=138, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=37.2365339578454, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=24.2990654205607, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=37.7777777777778, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.5098039215686, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=23.469387755102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=23.469387755102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=23.469387755102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=23.469387755102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=25.0481695568401, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45550774, Length=195, Percent_Identity=31.7948717948718, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648435, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648437, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648441, Length=130, Percent_Identity=36.1538461538462, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648439, Length=130, Percent_Identity=36.1538461538462, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356731, Length=509, Percent_Identity=22.0039292730845, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53404; Mature: 53404

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLVKLTISQAISGLKNKEFTATDLVTAHINQMDKYRCINAYITEIPELALQAAKKSDF
CCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LIQTNQARPIEGIPVSIKDLFCTKGILTTAASKMLQNFTPVYDATVYSKIINAGGIMLGK
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEC
GNMDEFAMGSANINSYFGNVINPWKAENDDIDLTPGGSSGGSSAAVAAFMAMAALGSDTG
CCCCHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
GSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQAGIITRTVEDAAIMLETMMGYDEK
CCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCC
DSTSLNVEVPNLRSAVNQPIKGMKIGIPYDLMENKSLSSEIITMWQNTINLLKDHGVEIV
CCCEEEEECCHHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
SISLPYINYALPVYYVLSSAEASSNLARYDGVRYGLRVEGKNSNINEIYELSRSEGFGAE
EEECCHHHHHHHHHHHHHCCHHHCCHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCCHH
VKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFNKVDCILIPSAPTAAFPLNSN
HHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCC
QDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
CCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
KALNLKFIPKGF
HHHCCCCCCCCC
>Mature Secondary Structure
MNNLVKLTISQAISGLKNKEFTATDLVTAHINQMDKYRCINAYITEIPELALQAAKKSDF
CCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LIQTNQARPIEGIPVSIKDLFCTKGILTTAASKMLQNFTPVYDATVYSKIINAGGIMLGK
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEC
GNMDEFAMGSANINSYFGNVINPWKAENDDIDLTPGGSSGGSSAAVAAFMAMAALGSDTG
CCCCHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
GSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQAGIITRTVEDAAIMLETMMGYDEK
CCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCC
DSTSLNVEVPNLRSAVNQPIKGMKIGIPYDLMENKSLSSEIITMWQNTINLLKDHGVEIV
CCCEEEEECCHHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
SISLPYINYALPVYYVLSSAEASSNLARYDGVRYGLRVEGKNSNINEIYELSRSEGFGAE
EEECCHHHHHHHHHHHHHCCHHHCCHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCCHH
VKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFNKVDCILIPSAPTAAFPLNSN
HHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCC
QDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
CCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
KALNLKFIPKGF
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA