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The map label for this gene is ntrX [C]

Identifier: 189183208

GI number: 189183208

Start: 312610

End: 314079

Strand: Reverse

Name: ntrX [C]

Synonym: OTT_0301

Alternate gene names: 189183208

Gene position: 314079-312610 (Counterclockwise)

Preceding gene: 189183209

Following gene: 189183207

Centisome position: 15.63

GC content: 33.27

Gene sequence:

>1470_bases
ATGGGCCATGTTTTAATAATAGATGATGAACACGATGTTAGAGCACTGCTATCAGATATTGTTAAAAGTAAAGGATTTAC
ACCAACAACTGCTGAAAATAGCTCTGAAGCACTAAGTATTCTAGATGTAAAACTACCTAAAATAGTAATTCTGGATGTAT
GGTTGCGTGGCAGTGATTTAGATGGATTAGGAATATTAGAAATTATAAGAAAAAAACATCCTTTAGTTCCTGTTATAATT
ATCAGTGGTCATGCTACAATTGAAACAGCAGTTACTGCTATCAAAATGGGAGCATATGATTATGTAGAGAAGCCATTTAC
AGCTGATAAAATAGTTACAGTATTAAAGCGAGCATGTGAAACAGCAAGGTTAAAAAAAGAAAACATTGATCTTAGAACCA
AAGTAATTGATAGATCAGAGATTATAGGGCAGTCACCTGCAATCAACAAACTTAAAGCAGAAATAGAGAAAATTTCGCCA
ACTTCTGGCAGAGTAATGATTACTGGTAATATTGGGTGTGGAAAGGAGCTAGTAGCCCAACTCATTCATCGGCGTTCAAA
ATATGCATTAGGACCATTTGTTACTTTTTGTCCAACAGCTCTTACTGAAGAACAAGCAAAATACGAATTATTTGGTGAGA
GAAATATTAATAACAACTCAGATTTTACTTATAAGGTTTGCGCGCTTGAAGCTGCCAATAATGGTACACTTTATATTGAT
GAAATTGCTGATTTACCTCTAAGCCTTCAATCTCGATTACTGAAATTTTTACAAGATAATAATTTCGAAAAAAATGGTTC
TAGTACATCAGTTAAACTTAATATTAGATTAATTAGCGCTACTGCTAAAAATATTCAAGAAGAAGTAACACTAGGAAGAT
TTAGACAGGATTTATATTACAGGCTAAATGTGATTCCAATTCATGTTCCATCACTTGCTAAGCGTAAGGAGGATATTCCT
CTGCTGGTGCAATATTTTGTTCAGCAGTTAGCAAAGTCGTCTGGATTGAAGGCCAGAAAGTTTTCAAATGAAGCAATTGC
AGCTTTGCAAGCTTATGATTGGCTAGGAAATATAAGACAATTGAGAAACGCAATAGAATGGACCTTAATCATGAATCCAC
TAACATCAAATAGCAACCATGAAATTGAGGTGGATATGCTTCCACCAGATATTATCAATAATAAGGCAGTAAGTATTATA
AAATCTAAGGCTAAGGTTAATATGATGTCTATGCCTTTAAGAAAAGCTAGAGAAGTATTTGAGCGTGAATATCTTGTTGC
TCAAATGAATAGATTTAATAATAATATTTCTAAAACTTCTGCTTTTGTTGGAATGGAAAGGTCAGCATTACATCGAAAGC
TTAAATTGCTGAACATTAATTGTAGTAATAAAGATGCACATAATAATGATTATCCGAGTGATGATAATTTACTTGCAACA
GGACAGCAAAATAAAGAGTGTCTAGCTTAA

Upstream 100 bases:

>100_bases
ATTTCTTATTGATTTATCTAGAATAGAGTACTAATTTAAGATTATAAGCAAATTTTAGTTAACCACTTAAATCATTAGTA
CAATTCTCAGGAGATAAATT

Downstream 100 bases:

>100_bases
CTGGTAATAATATATTCTTTTAATGATATATTTTAATGATAGACAGATGATTGTTCTTTAGGTATTTTAAAACACTTAAT
ATCAAAAAACGTAAATGCAT

Product: nitrogen assimilation regulatory protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MGHVLIIDDEHDVRALLSDIVKSKGFTPTTAENSSEALSILDVKLPKIVILDVWLRGSDLDGLGILEIIRKKHPLVPVII
ISGHATIETAVTAIKMGAYDYVEKPFTADKIVTVLKRACETARLKKENIDLRTKVIDRSEIIGQSPAINKLKAEIEKISP
TSGRVMITGNIGCGKELVAQLIHRRSKYALGPFVTFCPTALTEEQAKYELFGERNINNNSDFTYKVCALEAANNGTLYID
EIADLPLSLQSRLLKFLQDNNFEKNGSSTSVKLNIRLISATAKNIQEEVTLGRFRQDLYYRLNVIPIHVPSLAKRKEDIP
LLVQYFVQQLAKSSGLKARKFSNEAIAALQAYDWLGNIRQLRNAIEWTLIMNPLTSNSNHEIEVDMLPPDIINNKAVSII
KSKAKVNMMSMPLRKAREVFEREYLVAQMNRFNNNISKTSAFVGMERSALHRKLKLLNINCSNKDAHNNDYPSDDNLLAT
GQQNKECLA

Sequences:

>Translated_489_residues
MGHVLIIDDEHDVRALLSDIVKSKGFTPTTAENSSEALSILDVKLPKIVILDVWLRGSDLDGLGILEIIRKKHPLVPVII
ISGHATIETAVTAIKMGAYDYVEKPFTADKIVTVLKRACETARLKKENIDLRTKVIDRSEIIGQSPAINKLKAEIEKISP
TSGRVMITGNIGCGKELVAQLIHRRSKYALGPFVTFCPTALTEEQAKYELFGERNINNNSDFTYKVCALEAANNGTLYID
EIADLPLSLQSRLLKFLQDNNFEKNGSSTSVKLNIRLISATAKNIQEEVTLGRFRQDLYYRLNVIPIHVPSLAKRKEDIP
LLVQYFVQQLAKSSGLKARKFSNEAIAALQAYDWLGNIRQLRNAIEWTLIMNPLTSNSNHEIEVDMLPPDIINNKAVSII
KSKAKVNMMSMPLRKAREVFEREYLVAQMNRFNNNISKTSAFVGMERSALHRKLKLLNINCSNKDAHNNDYPSDDNLLAT
GQQNKECLA
>Mature_488_residues
GHVLIIDDEHDVRALLSDIVKSKGFTPTTAENSSEALSILDVKLPKIVILDVWLRGSDLDGLGILEIIRKKHPLVPVIII
SGHATIETAVTAIKMGAYDYVEKPFTADKIVTVLKRACETARLKKENIDLRTKVIDRSEIIGQSPAINKLKAEIEKISPT
SGRVMITGNIGCGKELVAQLIHRRSKYALGPFVTFCPTALTEEQAKYELFGERNINNNSDFTYKVCALEAANNGTLYIDE
IADLPLSLQSRLLKFLQDNNFEKNGSSTSVKLNIRLISATAKNIQEEVTLGRFRQDLYYRLNVIPIHVPSLAKRKEDIPL
LVQYFVQQLAKSSGLKARKFSNEAIAALQAYDWLGNIRQLRNAIEWTLIMNPLTSNSNHEIEVDMLPPDIINNKAVSIIK
SKAKVNMMSMPLRKAREVFEREYLVAQMNRFNNNISKTSAFVGMERSALHRKLKLLNINCSNKDAHNNDYPSDDNLLATG
QQNKECLA

Specific function: Member of the two-component regulatory system RBE_0312/RBE_0470 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=476, Percent_Identity=33.4033613445378, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788550, Length=463, Percent_Identity=34.1252699784017, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1790437, Length=459, Percent_Identity=33.7690631808279, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=31.140350877193, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=33.134328358209, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=32.5, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082117, Length=344, Percent_Identity=30.5232558139535, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=35.0393700787402, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787583, Length=318, Percent_Identity=32.0754716981132, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=30.9037900874636, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=27.5080906148867, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=28.2894736842105, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=30.3643724696356, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54826; Mature: 54695

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHVLIIDDEHDVRALLSDIVKSKGFTPTTAENSSEALSILDVKLPKIVILDVWLRGSDL
CCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCC
DGLGILEIIRKKHPLVPVIIISGHATIETAVTAIKMGAYDYVEKPFTADKIVTVLKRACE
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
TARLKKENIDLRTKVIDRSEIIGQSPAINKLKAEIEKISPTSGRVMITGNIGCGKELVAQ
HHHHHHCCCCHHHEEECHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
LIHRRSKYALGPFVTFCPTALTEEQAKYELFGERNINNNSDFTYKVCALEAANNGTLYID
HHHHHCCCCCCCHHHHCCCHHHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCEEEE
EIADLPLSLQSRLLKFLQDNNFEKNGSSTSVKLNIRLISATAKNIQEEVTLGRFRQDLYY
CHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHEE
RLNVIPIHVPSLAKRKEDIPLLVQYFVQQLAKSSGLKARKFSNEAIAALQAYDWLGNIRQ
EEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHHHHHH
LRNAIEWTLIMNPLTSNSNHEIEVDMLPPDIINNKAVSIIKSKAKVNMMSMPLRKAREVF
HHHHHEEEEEECCCCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EREYLVAQMNRFNNNISKTSAFVGMERSALHRKLKLLNINCSNKDAHNNDYPSDDNLLAT
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEC
GQQNKECLA
CCCCCCCCC
>Mature Secondary Structure 
GHVLIIDDEHDVRALLSDIVKSKGFTPTTAENSSEALSILDVKLPKIVILDVWLRGSDL
CCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCC
DGLGILEIIRKKHPLVPVIIISGHATIETAVTAIKMGAYDYVEKPFTADKIVTVLKRACE
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
TARLKKENIDLRTKVIDRSEIIGQSPAINKLKAEIEKISPTSGRVMITGNIGCGKELVAQ
HHHHHHCCCCHHHEEECHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
LIHRRSKYALGPFVTFCPTALTEEQAKYELFGERNINNNSDFTYKVCALEAANNGTLYID
HHHHHCCCCCCCHHHHCCCHHHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCEEEE
EIADLPLSLQSRLLKFLQDNNFEKNGSSTSVKLNIRLISATAKNIQEEVTLGRFRQDLYY
CHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHEE
RLNVIPIHVPSLAKRKEDIPLLVQYFVQQLAKSSGLKARKFSNEAIAALQAYDWLGNIRQ
EEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHHHHHH
LRNAIEWTLIMNPLTSNSNHEIEVDMLPPDIINNKAVSIIKSKAKVNMMSMPLRKAREVF
HHHHHEEEEEECCCCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EREYLVAQMNRFNNNISKTSAFVGMERSALHRKLKLLNINCSNKDAHNNDYPSDDNLLAT
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEC
GQQNKECLA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA