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The map label for this gene is spoT [H]

Identifier: 189183168

GI number: 189183168

Start: 259264

End: 261399

Strand: Reverse

Name: spoT [H]

Synonym: OTT_0261

Alternate gene names: 189183168

Gene position: 261399-259264 (Counterclockwise)

Preceding gene: 189183169

Following gene: 189183167

Centisome position: 13.01

GC content: 31.23

Gene sequence:

>2136_bases
ATGGTTAGTCGTGAAGACATTATTAATCAAGTATCTTCTTATGATCTAACATTTAATAAAACTCTTGTAAACAAAGCTAT
AGATTATGCTATTCACTATCACGGCAAACAAACGCGTGAGTCTGGTGATCCATATTACTATCACCCACTACATGTAGCTC
TTATTATTGCTCAAATGAAGCTTGATACCGTATCTGTAATTACTGCGCTATTACATGATACAGTAGAAGATACTGAACTA
ACTTTAAGTGATATTGAGCGTGAATTTGGCAAAGAAGTAGCAACGTTAGTAGATGGAGTTACTAAGCTTGCTAAACTTCG
ATTCCAGTCCTATCATCTACAACAAGCCAAGAACTTTCGTAAGCTACTGTTAGCTATAAGTAATGATATCAGGGTTTTAT
TGGTTAAATTAGCTGACCGTCTACATAATATGCGCACAATTGAGTCGATCAAGCTATTAAATAAGCGTATTAGAATAGCT
TTAGAAACAATAGAAATTTATGCACCACTAGCTGAAAGAATTGGAGCTCAGTATATTAAACTAGAATTACAGGATCTTGC
ATTTAAAGTAATGTATCCTAAAATCAGAAATGCCATTATTAGCAAAATCACGCAATATAATGATAATAACCGCATAACAT
CAGATACTATCATCATTGAAATTCAAAACACTTTAATGAAAGCTGGTATTAAAGCTCAGGTATCTGGCAGACATAAAGCA
CCTTACTCCATTTGGATGAAAATGAGACAAAAATGCATTGGGCTAGATCAGTTATCTGATCTTATGGCCTTCAGGATTAT
TGTCGATACTATTCCAGAATGTTATCAAGTCTTAGGTATTATTCATTCAAATTACAAAACAATACCTGGCAGCTTTCAAG
ATTTCATTAGTATTCCAAAGAATAACGGCTATCAATCAATACATACTACAATTATTGGCCCTCAGCAAAGAAGAATAGAA
GTTCAAATTAGAACTGTAAAAATGCATGAAATATCAGAGTTTGGAATAGCAGCACATTGGCAATATAAACAACATAAAGA
CAATGTAGAGCAGCATAAATGGGTTTTAGAATTATTAGATATATTAGGACGCTCTTATACAGCAACTGAATTTTTGCAGG
ATACAAAATTAGCAATGTATTATGACCAAGTTATCTGTTTTACTCCAGAAGGAAAACTGATTGCTTTACCTAAAGGAGCT
ACTGTAGTAGACTTTGCTTATAAAGTACACTCAGAATTAGGAAATAAATGCATAGGAGCAAAAATTAGCAACAAAGTTGT
ACCATTAGATACACAACTGCAAAATGGAGATCAAGTAGAAATTATAACATCGCATACACAATTCCCTATTCCTCAATGGA
AAACATTTGCTGTAACAGGTAAAGCTCAATCAGAAATTCGTAAATTTATACGTAGTCAAGCATACAAAAAATACATTGAT
TTAGGCAAAGAAATACTAATACAAACATTAAAAAAAATACAAGTTGCTAATATTAATGTATGCATTGCTAAAATCGCTCA
CGAGCTAAATAAAAAGAATGTAGAAGAAGTATTTTTCTCAATTGGAAGTGAATTATTATCAAAGAAAGAAATTATCAAAA
TTATTACTTCAATATCAGACCCAGTTAACCCAGTAACATTAAATAAAGCGTATTGTCTAAGTAATGAACTATCACCAATT
GCTATATCTGATAATAATAAGATTGATAAAGTCAACCAAAACTTACCTATAGATTTTGCTAAATGCTGTTACCCATTGCC
TGGTGACTTAATTATTGGTCTTTGCACAAAAACAGAAATAGTAATTCACACTACCTACTGCACTATTGCTTCTAATTATG
TTTTGGAATCTATAAGATTATTAGACTTTAGCTGGACTAGTAACGCTCAAAAGATGTTCTATACTTCAAGTGTGCAATTA
TCTGTACGTAATAAAATAGGTAGCTTAGCCAGTATTACTACTATACTTGAAAATAATAATATTAACATCTGTAATATCAA
AACTACTAATTGTACTCAGAGCACAACTCAAATAATAATAGATATAGAAATATCTACACTTGAGCAGTTAAATAAAATAA
TAAATATATTACAGTCATCTCAAGACATAATATCTGTTAAAAGAGTATCTACATAA

Upstream 100 bases:

>100_bases
ACTCTGAATTTGACAACCAGCAAATATATCTGAGCAGTGAGAACTTAAGTATTATTAATGATGATGATACAAGAAAAATA
TAAAAAACAACTTAACAGCT

Downstream 100 bases:

>100_bases
TGGTTTTTATATGATATATGGAATAGGAACTGATATAATACACATACCAAGAATTGAACAACTAATTAATAAATACTCAA
CTAAATTTATTAATCGTATT

Product: SpoT protein

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MVSREDIINQVSSYDLTFNKTLVNKAIDYAIHYHGKQTRESGDPYYYHPLHVALIIAQMKLDTVSVITALLHDTVEDTEL
TLSDIEREFGKEVATLVDGVTKLAKLRFQSYHLQQAKNFRKLLLAISNDIRVLLVKLADRLHNMRTIESIKLLNKRIRIA
LETIEIYAPLAERIGAQYIKLELQDLAFKVMYPKIRNAIISKITQYNDNNRITSDTIIIEIQNTLMKAGIKAQVSGRHKA
PYSIWMKMRQKCIGLDQLSDLMAFRIIVDTIPECYQVLGIIHSNYKTIPGSFQDFISIPKNNGYQSIHTTIIGPQQRRIE
VQIRTVKMHEISEFGIAAHWQYKQHKDNVEQHKWVLELLDILGRSYTATEFLQDTKLAMYYDQVICFTPEGKLIALPKGA
TVVDFAYKVHSELGNKCIGAKISNKVVPLDTQLQNGDQVEIITSHTQFPIPQWKTFAVTGKAQSEIRKFIRSQAYKKYID
LGKEILIQTLKKIQVANINVCIAKIAHELNKKNVEEVFFSIGSELLSKKEIIKIITSISDPVNPVTLNKAYCLSNELSPI
AISDNNKIDKVNQNLPIDFAKCCYPLPGDLIIGLCTKTEIVIHTTYCTIASNYVLESIRLLDFSWTSNAQKMFYTSSVQL
SVRNKIGSLASITTILENNNINICNIKTTNCTQSTTQIIIDIEISTLEQLNKIINILQSSQDIISVKRVST

Sequences:

>Translated_711_residues
MVSREDIINQVSSYDLTFNKTLVNKAIDYAIHYHGKQTRESGDPYYYHPLHVALIIAQMKLDTVSVITALLHDTVEDTEL
TLSDIEREFGKEVATLVDGVTKLAKLRFQSYHLQQAKNFRKLLLAISNDIRVLLVKLADRLHNMRTIESIKLLNKRIRIA
LETIEIYAPLAERIGAQYIKLELQDLAFKVMYPKIRNAIISKITQYNDNNRITSDTIIIEIQNTLMKAGIKAQVSGRHKA
PYSIWMKMRQKCIGLDQLSDLMAFRIIVDTIPECYQVLGIIHSNYKTIPGSFQDFISIPKNNGYQSIHTTIIGPQQRRIE
VQIRTVKMHEISEFGIAAHWQYKQHKDNVEQHKWVLELLDILGRSYTATEFLQDTKLAMYYDQVICFTPEGKLIALPKGA
TVVDFAYKVHSELGNKCIGAKISNKVVPLDTQLQNGDQVEIITSHTQFPIPQWKTFAVTGKAQSEIRKFIRSQAYKKYID
LGKEILIQTLKKIQVANINVCIAKIAHELNKKNVEEVFFSIGSELLSKKEIIKIITSISDPVNPVTLNKAYCLSNELSPI
AISDNNKIDKVNQNLPIDFAKCCYPLPGDLIIGLCTKTEIVIHTTYCTIASNYVLESIRLLDFSWTSNAQKMFYTSSVQL
SVRNKIGSLASITTILENNNINICNIKTTNCTQSTTQIIIDIEISTLEQLNKIINILQSSQDIISVKRVST
>Mature_711_residues
MVSREDIINQVSSYDLTFNKTLVNKAIDYAIHYHGKQTRESGDPYYYHPLHVALIIAQMKLDTVSVITALLHDTVEDTEL
TLSDIEREFGKEVATLVDGVTKLAKLRFQSYHLQQAKNFRKLLLAISNDIRVLLVKLADRLHNMRTIESIKLLNKRIRIA
LETIEIYAPLAERIGAQYIKLELQDLAFKVMYPKIRNAIISKITQYNDNNRITSDTIIIEIQNTLMKAGIKAQVSGRHKA
PYSIWMKMRQKCIGLDQLSDLMAFRIIVDTIPECYQVLGIIHSNYKTIPGSFQDFISIPKNNGYQSIHTTIIGPQQRRIE
VQIRTVKMHEISEFGIAAHWQYKQHKDNVEQHKWVLELLDILGRSYTATEFLQDTKLAMYYDQVICFTPEGKLIALPKGA
TVVDFAYKVHSELGNKCIGAKISNKVVPLDTQLQNGDQVEIITSHTQFPIPQWKTFAVTGKAQSEIRKFIRSQAYKKYID
LGKEILIQTLKKIQVANINVCIAKIAHELNKKNVEEVFFSIGSELLSKKEIIKIITSISDPVNPVTLNKAYCLSNELSPI
AISDNNKIDKVNQNLPIDFAKCCYPLPGDLIIGLCTKTEIVIHTTYCTIASNYVLESIRLLDFSWTSNAQKMFYTSSVQL
SVRNKIGSLASITTILENNNINICNIKTTNCTQSTTQIIIDIEISTLEQLNKIINILQSSQDIISVKRVST

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=130, Percent_Identity=38.4615384615385, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790082, Length=685, Percent_Identity=37.3722627737226, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1789147, Length=694, Percent_Identity=31.9884726224784, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25143118, Length=130, Percent_Identity=36.1538461538462, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80784; Mature: 80784

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSREDIINQVSSYDLTFNKTLVNKAIDYAIHYHGKQTRESGDPYYYHPLHVALIIAQMK
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHEECCCCCCCCCCCEEECHHHHHHHHHHHH
LDTVSVITALLHDTVEDTELTLSDIEREFGKEVATLVDGVTKLAKLRFQSYHLQQAKNFR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLLAISNDIRVLLVKLADRLHNMRTIESIKLLNKRIRIALETIEIYAPLAERIGAQYIK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LELQDLAFKVMYPKIRNAIISKITQYNDNNRITSDTIIIEIQNTLMKAGIKAQVSGRHKA
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEHHHHHHCCCEEEECCCCCC
PYSIWMKMRQKCIGLDQLSDLMAFRIIVDTIPECYQVLGIIHSNYKTIPGSFQDFISIPK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
NNGYQSIHTTIIGPQQRRIEVQIRTVKMHEISEFGIAAHWQYKQHKDNVEQHKWVLELLD
CCCCCEEEEEEECCCCEEEEEEEEEEEEHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHH
ILGRSYTATEFLQDTKLAMYYDQVICFTPEGKLIALPKGATVVDFAYKVHSELGNKCIGA
HHCCCCHHHHHHHHCHHEEEECEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCEECC
KISNKVVPLDTQLQNGDQVEIITSHTQFPIPQWKTFAVTGKAQSEIRKFIRSQAYKKYID
CCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
LGKEILIQTLKKIQVANINVCIAKIAHELNKKNVEEVFFSIGSELLSKKEIIKIITSISD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVNPVTLNKAYCLSNELSPIAISDNNKIDKVNQNLPIDFAKCCYPLPGDLIIGLCTKTEI
CCCCEECCCEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHEEEEECCCEE
VIHTTYCTIASNYVLESIRLLDFSWTSNAQKMFYTSSVQLSVRNKIGSLASITTILENNN
EEEEEHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEEEEEEHHHCCCHHHHHHEEECCC
INICNIKTTNCTQSTTQIIIDIEISTLEQLNKIINILQSSQDIISVKRVST
EEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHEECCC
>Mature Secondary Structure
MVSREDIINQVSSYDLTFNKTLVNKAIDYAIHYHGKQTRESGDPYYYHPLHVALIIAQMK
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHEECCCCCCCCCCCEEECHHHHHHHHHHHH
LDTVSVITALLHDTVEDTELTLSDIEREFGKEVATLVDGVTKLAKLRFQSYHLQQAKNFR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLLAISNDIRVLLVKLADRLHNMRTIESIKLLNKRIRIALETIEIYAPLAERIGAQYIK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LELQDLAFKVMYPKIRNAIISKITQYNDNNRITSDTIIIEIQNTLMKAGIKAQVSGRHKA
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEHHHHHHCCCEEEECCCCCC
PYSIWMKMRQKCIGLDQLSDLMAFRIIVDTIPECYQVLGIIHSNYKTIPGSFQDFISIPK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
NNGYQSIHTTIIGPQQRRIEVQIRTVKMHEISEFGIAAHWQYKQHKDNVEQHKWVLELLD
CCCCCEEEEEEECCCCEEEEEEEEEEEEHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHH
ILGRSYTATEFLQDTKLAMYYDQVICFTPEGKLIALPKGATVVDFAYKVHSELGNKCIGA
HHCCCCHHHHHHHHCHHEEEECEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCEECC
KISNKVVPLDTQLQNGDQVEIITSHTQFPIPQWKTFAVTGKAQSEIRKFIRSQAYKKYID
CCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
LGKEILIQTLKKIQVANINVCIAKIAHELNKKNVEEVFFSIGSELLSKKEIIKIITSISD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVNPVTLNKAYCLSNELSPIAISDNNKIDKVNQNLPIDFAKCCYPLPGDLIIGLCTKTEI
CCCCEECCCEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHEEEEECCCEE
VIHTTYCTIASNYVLESIRLLDFSWTSNAQKMFYTSSVQLSVRNKIGSLASITTILENNN
EEEEEHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEEEEEEHHHCCCHHHHHHEEECCC
INICNIKTTNCTQSTTQIIIDIEISTLEQLNKIINILQSSQDIISVKRVST
EEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA