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The map label for this gene is leuS [H]

Identifier: 189183152

GI number: 189183152

Start: 232416

End: 234935

Strand: Reverse

Name: leuS [H]

Synonym: OTT_0245

Alternate gene names: 189183152

Gene position: 234935-232416 (Counterclockwise)

Preceding gene: 189183153

Following gene: 189183151

Centisome position: 11.69

GC content: 31.35

Gene sequence:

>2520_bases
ATGGACTTAGTAAAAATTGAAAAAAAGTGGCAAGAAATCTGGGAGCAGAATGAGGTATTTAAAGTAAAAGAGATTTTAAA
TAAGATCAAACGTTATATATTACCGATGTTTCCTTATCCATCTGGTAAGGCTCATGTAGGACATGTACGTAACTATACAA
TATGTGATGTTATTGCGAGGTTTGAACGATCTCAAGGGCATAATGTATTGCATGCTATGGGTTGGGATGCATTTGGATTA
CCAGCCGAAAATGCTGCAATGCAAAATAATACTCACCCAAATAGTTGGGTAAATAGCAACGTTGATGTTATGAAGCGACA
ACTTAAGGCAATTGGATTGTCTTATGATTGGAGTCGCGAAATAATTACTTGCTCACCACAATATTATGTTCATGAACAGA
GATTTTTTCTTGAAATGCTGAAAAAAGGTCTTGTATATCAAAAAGAATCATTAGTTAATTGGGATCCTGTTGATCAAACA
GTTCTTGCTAATGAACAAGTAATTAATGGCAAAGGATGGAGATCAGGAGCAATAATTGAATATCGTAATTTAAAGCAGTG
GTTTATTAAAATTACTAACTATGCTGATGCTTTATTAAAAGGCCTTGATAGCTTACAAAGTTGGCCAGAAAGTGTAAAAA
CAATGCAAAAAAAATGGATTGGTCAGTCAAAAGGAATTAATATAGATTTTCAACTTAAAGGAATAGAAGCTTCAATTAAG
GTATTTTCAACTAAGCCTGAAACTTTGTTTGGAGCTTCTTTTATAGCATTAAGTTATAATCATACTTTAGTGCAGCAATA
TGTACATACCACACCAGAAATTCAAGAATTTATTGATAAATGCAGTAATGTTGGCACCTCAAATGTTAATATTGATAAAA
TGAGTAAACTTGCTGTACTAACAAATCTTAAGGTAATTCATCCACTAAACAGTAGTATAGAATTGCCAGTAATTCTTAGT
AATTTTGTCTTAATGGATTATGGTACTGGAGCATTATTTGGATGTCCAGCACACGATGAGAGGGATCATGAGATTGCAAA
ATTATTAAAGTTGAATATTAAGCAGGTCATAACCTCAACTGAAAGAGATATTGATGTGCTTAAAGAAGCATATGTAGGTG
ATGGAATAATGATTAACTCATTTCATTTGAATGGATTAACTACTGCTGAAGCTCGGCAAAAAGTTATTAATGAACTGCAG
CATAAAAATATAGGACAACAAGTTACGAATTACAAATTAAAAGATTGGGGGATTTCTCGGCAAAGATTTTGGGGATGTCC
TATTCCGATTATCCACTGTAAAAGCTGTGGAGCAGTACCAGTACCTTATGAAGACTTGCCAGTAATATTACCAGAACATG
GAGTTGAATTTACTGGTAAAGGTAATCCATTAGATAATCATCCTTCCTGGAAATATGTTAAATGTCCTAAATGCCACCTA
GATGCAGTTAGAGAAACAGATACATTTGATACTTTTTTTGAATCATCCTGGTATTTCGCCAGATTTTGTAATTCTACGAG
TGATGATATGGTCGATGCTAAAGCTGCAAAATATTGGTTGCCAGTAGATCAATATATTGGAGGTATTGAGCATGCAGTAA
TGCATTTATTATATGCTAGATTTATAACTAGAGTAATGTATGATCTGAAGTATATAGATATTCAAGAACCTTTTACATCG
TTGATAACTCAAGGCATGGTTCTGCATAGAACTTATCAGGACCAAAGTAATAATTGGGTGTATCCAAATGAAGTTGAAGT
TGATTCAAATGGAAAATTAAGATGTAAGGCAGATTCACAATATGTGACAGTAGGTAAATTAGAAAAGATGAGCAAGTCTA
AAAAGAATGTTGTAGATTTAGAATTGGTACTTAAGCTATATGGCGCTGATGTAGCACGTATGTTTGTTTTATCAGATACA
CCTCCTGAAAAAGATTTAGAATGGTCAACTGAAGGTATTGAAGGTTGTTATAAATTTGTTCAAAAGTTATATAACTTTGC
ATTGAAGCTTAAGAATATAAATTTAACTGATAATAAAATTGATAAAGTTTTGCTTAACAAAACGCACAAAACTATTAAAA
ACGTTACACAAGATATTATAAGTTGCAGGCTAAATAAAGCTATAGCTAGATTAAGAGAATTATATAATCTTATATTTAAA
ATGTCTGAATTAACAGTTCAAATAAAAGAGAGTTTTTTAATTTTAATAAGATTGTTTAATCCATTTATTCCACATTTATC
AGAGGAAATATGGAGTTTGCTTTCAGACGGAGAGAAAATGCTAGTTGAATTACCATGGCCTAAATATGAAGAAAAATATA
TTCATGAAGAAGAGCATATTACTATTGCTATACAAATTAATGGTAAGTTAAGATCATTATATAATTGTCTAATTGATACT
CCAGAACATGACGTTCAGTCTGCAATTTTAAAATTAGAACAGGTAAAAAAGCATATTGGCGATAAAGAAGTAAGAAAATG
TATTTTTATTCCAAATAAATTAATTAATATAATTGTATGA

Upstream 100 bases:

>100_bases
AAAGCTAATCAAGAATTAGCAAGTACAAGTATGGTATTCCAACATATTGCAAAAAACTAAAATTGGCATTTGTTAAGTAA
GCTATTTATGGAAAAATTTT

Downstream 100 bases:

>100_bases
TAATACAGGATTATTACTAGTGAAGTTTACGTCTTATAATATTGCTGCATTATGGTCTTATATTAAAAAAGGTGATATTA
GAGCAATAGCAGTTTATGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 839; Mature: 839

Protein sequence:

>839_residues
MDLVKIEKKWQEIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTHPNSWVNSNVDVMKRQLKAIGLSYDWSREIITCSPQYYVHEQRFFLEMLKKGLVYQKESLVNWDPVDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLQSWPESVKTMQKKWIGQSKGINIDFQLKGIEASIK
VFSTKPETLFGASFIALSYNHTLVQQYVHTTPEIQEFIDKCSNVGTSNVNIDKMSKLAVLTNLKVIHPLNSSIELPVILS
NFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERDIDVLKEAYVGDGIMINSFHLNGLTTAEARQKVINELQ
HKNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHPSWKYVKCPKCHL
DAVRETDTFDTFFESSWYFARFCNSTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTS
LITQGMVLHRTYQDQSNNWVYPNEVEVDSNGKLRCKADSQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDT
PPEKDLEWSTEGIEGCYKFVQKLYNFALKLKNINLTDNKIDKVLLNKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFK
MSELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSDGEKMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDT
PEHDVQSAILKLEQVKKHIGDKEVRKCIFIPNKLINIIV

Sequences:

>Translated_839_residues
MDLVKIEKKWQEIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTHPNSWVNSNVDVMKRQLKAIGLSYDWSREIITCSPQYYVHEQRFFLEMLKKGLVYQKESLVNWDPVDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLQSWPESVKTMQKKWIGQSKGINIDFQLKGIEASIK
VFSTKPETLFGASFIALSYNHTLVQQYVHTTPEIQEFIDKCSNVGTSNVNIDKMSKLAVLTNLKVIHPLNSSIELPVILS
NFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERDIDVLKEAYVGDGIMINSFHLNGLTTAEARQKVINELQ
HKNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHPSWKYVKCPKCHL
DAVRETDTFDTFFESSWYFARFCNSTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTS
LITQGMVLHRTYQDQSNNWVYPNEVEVDSNGKLRCKADSQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDT
PPEKDLEWSTEGIEGCYKFVQKLYNFALKLKNINLTDNKIDKVLLNKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFK
MSELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSDGEKMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDT
PEHDVQSAILKLEQVKKHIGDKEVRKCIFIPNKLINIIV
>Mature_839_residues
MDLVKIEKKWQEIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTHPNSWVNSNVDVMKRQLKAIGLSYDWSREIITCSPQYYVHEQRFFLEMLKKGLVYQKESLVNWDPVDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLQSWPESVKTMQKKWIGQSKGINIDFQLKGIEASIK
VFSTKPETLFGASFIALSYNHTLVQQYVHTTPEIQEFIDKCSNVGTSNVNIDKMSKLAVLTNLKVIHPLNSSIELPVILS
NFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERDIDVLKEAYVGDGIMINSFHLNGLTTAEARQKVINELQ
HKNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHPSWKYVKCPKCHL
DAVRETDTFDTFFESSWYFARFCNSTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTS
LITQGMVLHRTYQDQSNNWVYPNEVEVDSNGKLRCKADSQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDT
PPEKDLEWSTEGIEGCYKFVQKLYNFALKLKNINLTDNKIDKVLLNKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFK
MSELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSDGEKMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDT
PEHDVQSAILKLEQVKKHIGDKEVRKCIFIPNKLINIIV

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=877, Percent_Identity=36.4880273660205, Blast_Score=528, Evalue=1e-149,
Organism=Homo sapiens, GI46852147, Length=339, Percent_Identity=25.0737463126844, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=43.0232558139535, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=386, Percent_Identity=24.6113989637306, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71997510, Length=248, Percent_Identity=40.7258064516129, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71997517, Length=248, Percent_Identity=40.7258064516129, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=27.9569892473118, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17510661, Length=922, Percent_Identity=20.6073752711497, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71980946, Length=142, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323414, Length=875, Percent_Identity=35.5428571428571, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6321531, Length=429, Percent_Identity=22.8438228438228, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325217, Length=144, Percent_Identity=27.0833333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355409, Length=873, Percent_Identity=34.020618556701, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281366294, Length=682, Percent_Identity=22.4340175953079, Blast_Score=84, Evalue=6e-16,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96733; Mature: 96733

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVKIEKKWQEIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIAR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
FERSQGHNVLHAMGWDAFGLPAENAAMQNNTHPNSWVNSNVDVMKRQLKAIGLSYDWSRE
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
IITCSPQYYVHEQRFFLEMLKKGLVYQKESLVNWDPVDQTVLANEQVINGKGWRSGAIIE
EEEECCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHCCCEEECCCCCCCCCEEE
YRNLKQWFIKITNYADALLKGLDSLQSWPESVKTMQKKWIGQSKGINIDFQLKGIEASIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEE
VFSTKPETLFGASFIALSYNHTLVQQYVHTTPEIQEFIDKCSNVGTSNVNIDKMSKLAVL
EEECCCCHHHCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TNLKVIHPLNSSIELPVILSNFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITST
HCCEEEECCCCCCCCCCHHHCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCC
ERDIDVLKEAYVGDGIMINSFHLNGLTTAEARQKVINELQHKNIGQQVTNYKLKDWGISR
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCH
QRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHPSWKYVKCPKCHL
HHCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCHHH
DAVRETDTFDTFFESSWYFARFCNSTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYAR
HHHHCCCHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHH
FITRVMYDLKYIDIQEPFTSLITQGMVLHRTYQDQSNNWVYPNEVEVDSNGKLRCKADSQ
HHHHHHHHHHEEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCEEEECCCC
YVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTPPEKDLEWSTEGIEGCYKFV
EEEHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
QKLYNFALKLKNINLTDNKIDKVLLNKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFK
HHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSDGEKMLVELPWPKYEEKYIHEEEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEE
TIAIQINGKLRSLYNCLIDTPEHDVQSAILKLEQVKKHIGDKEVRKCIFIPNKLINIIV
EEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MDLVKIEKKWQEIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIAR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
FERSQGHNVLHAMGWDAFGLPAENAAMQNNTHPNSWVNSNVDVMKRQLKAIGLSYDWSRE
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
IITCSPQYYVHEQRFFLEMLKKGLVYQKESLVNWDPVDQTVLANEQVINGKGWRSGAIIE
EEEECCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHCCCEEECCCCCCCCCEEE
YRNLKQWFIKITNYADALLKGLDSLQSWPESVKTMQKKWIGQSKGINIDFQLKGIEASIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEE
VFSTKPETLFGASFIALSYNHTLVQQYVHTTPEIQEFIDKCSNVGTSNVNIDKMSKLAVL
EEECCCCHHHCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TNLKVIHPLNSSIELPVILSNFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITST
HCCEEEECCCCCCCCCCHHHCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCC
ERDIDVLKEAYVGDGIMINSFHLNGLTTAEARQKVINELQHKNIGQQVTNYKLKDWGISR
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCH
QRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHPSWKYVKCPKCHL
HHCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCHHH
DAVRETDTFDTFFESSWYFARFCNSTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYAR
HHHHCCCHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHH
FITRVMYDLKYIDIQEPFTSLITQGMVLHRTYQDQSNNWVYPNEVEVDSNGKLRCKADSQ
HHHHHHHHHHEEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCEEEECCCC
YVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTPPEKDLEWSTEGIEGCYKFV
EEEHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
QKLYNFALKLKNINLTDNKIDKVLLNKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFK
HHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSDGEKMLVELPWPKYEEKYIHEEEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEE
TIAIQINGKLRSLYNCLIDTPEHDVQSAILKLEQVKKHIGDKEVRKCIFIPNKLINIIV
EEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA