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The map label for this gene is 189183153

Identifier: 189183153

GI number: 189183153

Start: 235555

End: 236286

Strand: Reverse

Name: 189183153

Synonym: OTT_0246

Alternate gene names: NA

Gene position: 236286-235555 (Counterclockwise)

Preceding gene: 189183154

Following gene: 189183152

Centisome position: 11.76

GC content: 29.37

Gene sequence:

>732_bases
ATGGTTAAATTAGATATATTGAACATAATCGACCTTAAATGGAAGGGGGTAAGGTTATTGATGTTTTGCTATCTAGCAAT
TAACAATATACAAGCAAATAGCATTACTAATTATAACAACAAATCAAATTCATATATTAATATAAGCTATAGTAATGAGA
TTCACTCATGCCAAGTCACCAATAATTATGAGCCAGAAGTTTTCAATATCACAAATAATTTATTACGGCAAGTTGGACAA
AATCCTCGTATCAAAGGTACAATAGTAGTTATAAAAGGCACTGTCATTGATAGGCATTGTATTCCAGTTCCTGACGCAAA
AGTCTATCTATGGCAAGTTGATAGTAATGGTAAGTATCCATATACTCCTCTAAAAAATTCTATTGATCAATCTCTAATTA
GTAATAATAATTTAGAGAGTACTTTTACTGGTTCAGGTATTACCACCACAAATAATAATGGTAATTTTTATTTTATTACT
ATCTATCCTGCTGTAGTACACAATATTTTACCTCATTTTAACTTCAGAGCTGAACATACAGAGCTTGGCAGTTTACAAAC
AGTACATATAATCAAGAATGATATAAGATTTAAGATATCATGTAATGATCATATGCTCTACAATAATATTGACAACTATT
TTATTGTAGAGCATGTAACACTAGTCATGCCATTAGCAAGTAAACTAAGGAGATATATTCTACATGCTAGTACTAAACAT
AATGCAAATTAA

Upstream 100 bases:

>100_bases
ACGTTATATCTAATATTATTAGAATTAAATATTGATGCAATATGACAAATATGATAGAGTATCTTTTATTGATAGTAAAA
TGGATTTCATGTATTTATAA

Downstream 100 bases:

>100_bases
AATAGGCCAAAAAAGTTAATCTAAGCTTTAAATTTATATTGATATAAAAAGCATAGTGTTAAAAATGTACAAAATTGAAA
GTGAATAAGTTCGAATTAGA

Product: protocatechuate 3,4-dioxygenase beta subunit

Products: 3-carboxy-cis,cis-muconate

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVKLDILNIIDLKWKGVRLLMFCYLAINNIQANSITNYNNKSNSYINISYSNEIHSCQVTNNYEPEVFNITNNLLRQVGQ
NPRIKGTIVVIKGTVIDRHCIPVPDAKVYLWQVDSNGKYPYTPLKNSIDQSLISNNNLESTFTGSGITTTNNNGNFYFIT
IYPAVVHNILPHFNFRAEHTELGSLQTVHIIKNDIRFKISCNDHMLYNNIDNYFIVEHVTLVMPLASKLRRYILHASTKH
NAN

Sequences:

>Translated_243_residues
MVKLDILNIIDLKWKGVRLLMFCYLAINNIQANSITNYNNKSNSYINISYSNEIHSCQVTNNYEPEVFNITNNLLRQVGQ
NPRIKGTIVVIKGTVIDRHCIPVPDAKVYLWQVDSNGKYPYTPLKNSIDQSLISNNNLESTFTGSGITTTNNNGNFYFIT
IYPAVVHNILPHFNFRAEHTELGSLQTVHIIKNDIRFKISCNDHMLYNNIDNYFIVEHVTLVMPLASKLRRYILHASTKH
NAN
>Mature_243_residues
MVKLDILNIIDLKWKGVRLLMFCYLAINNIQANSITNYNNKSNSYINISYSNEIHSCQVTNNYEPEVFNITNNLLRQVGQ
NPRIKGTIVVIKGTVIDRHCIPVPDAKVYLWQVDSNGKYPYTPLKNSIDQSLISNNNLESTFTGSGITTTNNNGNFYFIT
IYPAVVHNILPHFNFRAEHTELGSLQTVHIIKNDIRFKISCNDHMLYNNIDNYFIVEHVTLVMPLASKLRRYILHASTKH
NAN

Specific function: Unknown

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: 1.13.11.3

Molecular weight: Translated: 27861; Mature: 27861

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLDILNIIDLKWKGVRLLMFCYLAINNIQANSITNYNNKSNSYINISYSNEIHSCQVT
CEEEEEEEEEECCCCCHHHHHHHHHHHCCEECCCEECCCCCCCCEEEEEECCCEEEEEEC
NNYEPEVFNITNNLLRQVGQNPRIKGTIVVIKGTVIDRHCIPVPDAKVYLWQVDSNGKYP
CCCCCCEEEHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
YTPLKNSIDQSLISNNNLESTFTGSGITTTNNNGNFYFITIYPAVVHNILPHFNFRAEHT
CCCHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCEEEEEEEHHHHHHHHCCCCCEECCC
ELGSLQTVHIIKNDIRFKISCNDHMLYNNIDNYFIVEHVTLVMPLASKLRRYILHASTKH
CCCCEEEEEEEECCEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCC
NAN
CCC
>Mature Secondary Structure
MVKLDILNIIDLKWKGVRLLMFCYLAINNIQANSITNYNNKSNSYINISYSNEIHSCQVT
CEEEEEEEEEECCCCCHHHHHHHHHHHCCEECCCEECCCCCCCCEEEEEECCCEEEEEEC
NNYEPEVFNITNNLLRQVGQNPRIKGTIVVIKGTVIDRHCIPVPDAKVYLWQVDSNGKYP
CCCCCCEEEHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
YTPLKNSIDQSLISNNNLESTFTGSGITTTNNNGNFYFITIYPAVVHNILPHFNFRAEHT
CCCHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCEEEEEEEHHHHHHHHCCCCCEECCC
ELGSLQTVHIIKNDIRFKISCNDHMLYNNIDNYFIVEHVTLVMPLASKLRRYILHASTKH
CCCCEEEEEEEECCEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCC
NAN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3,4-dihydroxybenzoate; O2

Specific reaction: 3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]