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The map label for this gene is lipA [H]

Identifier: 189182993

GI number: 189182993

Start: 90657

End: 91565

Strand: Direct

Name: lipA [H]

Synonym: OTT_0086

Alternate gene names: 189182993

Gene position: 90657-91565 (Clockwise)

Preceding gene: 189182992

Following gene: 189182994

Centisome position: 4.51

GC content: 34.98

Gene sequence:

>909_bases
ATGTGTAGCGATAAGAATATTACAGCTACAGCCTTAGTAAGGCCTAGCTGGTTAAGAGTTAAAGCTCCTTTTTCTGATGA
GTATCAAAGTACTAATGAGCTTATCAAGAGCTTAAAGTTAAATACTGTATGTAAGGAGGCAGCATGTCCTAATATTGGTG
AATGCTGGTCTAAAAAGCATGCAACGGTTATGATTCTTGGTTCCATTTGTACTAGAGCTTGCGCATTTTGTAATGTTAGT
ACAGGAAAACCAGAACAAGTAGATGAATATGAGCCATATAGGCTATCTGAAGCAGTAATGAAATTAGGCCTTAAGCATGT
AGTAATTACTTCTGTAGATAGGGACGATATTAGTGATGGAGGAGCTAGTCACTTTGCTAAGTGTATAACTTATATTAGGG
AAAGATCTCCTAGTACTTCAATAGAGGTACTAACGCCAGATTTTTTACGTAAACATGACGCTTGGAAGATAGTTGCAAAA
GCACGACCAGATGTATATAATCATAATATTGAAACTGTGCCATCATTATATTTAAAAGTCAGACCTGGAGCTCGTTATTA
TAACTCATTAAATTTGCTACATCAGGTTAAGATATTTGATAGTAGTATTTTTACTAAATCTGGTATTATGGTTGGTTTAG
GAGAAACTAAACATGAAGTACTACAAGTAATGGATGATTTAAGAGCTGCCGAAGTAGATTTTTTAACTATTGGTCAATAT
TTACGTCCTAGTGCTCGACATATAGATGTAGACCGATATGTTGCACCTGATGAGTTTGATTATTATGCCAGAGTTGCTAA
GTCTAAGGGGTTTTTAATGGTTTCTGCAAGCCCTCTAACTCGGTCTTCATATCATGCTGGAGAACATTTTGAAAAATTAA
AACAAATGAGGCTACAAAATATAATATAA

Upstream 100 bases:

>100_bases
CTAGACAGCTATATTGAGTTATTTCAAAATAACATACGTAAATTGTTTTTTGGTAGTAAAATAAATGGTGTAATGGCATT
GTATTGTTAAGTTATTAATT

Downstream 100 bases:

>100_bases
AAATTTTTATATAACAAATAATGCTGTTTTTTAATAAAGCTAAGATTCTACCTTATAGTGCAAAACATCTTTACCAACTT
GTGCTTGATATTGAATCTTA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MCSDKNITATALVRPSWLRVKAPFSDEYQSTNELIKSLKLNTVCKEAACPNIGECWSKKHATVMILGSICTRACAFCNVS
TGKPEQVDEYEPYRLSEAVMKLGLKHVVITSVDRDDISDGGASHFAKCITYIRERSPSTSIEVLTPDFLRKHDAWKIVAK
ARPDVYNHNIETVPSLYLKVRPGARYYNSLNLLHQVKIFDSSIFTKSGIMVGLGETKHEVLQVMDDLRAAEVDFLTIGQY
LRPSARHIDVDRYVAPDEFDYYARVAKSKGFLMVSASPLTRSSYHAGEHFEKLKQMRLQNII

Sequences:

>Translated_302_residues
MCSDKNITATALVRPSWLRVKAPFSDEYQSTNELIKSLKLNTVCKEAACPNIGECWSKKHATVMILGSICTRACAFCNVS
TGKPEQVDEYEPYRLSEAVMKLGLKHVVITSVDRDDISDGGASHFAKCITYIRERSPSTSIEVLTPDFLRKHDAWKIVAK
ARPDVYNHNIETVPSLYLKVRPGARYYNSLNLLHQVKIFDSSIFTKSGIMVGLGETKHEVLQVMDDLRAAEVDFLTIGQY
LRPSARHIDVDRYVAPDEFDYYARVAKSKGFLMVSASPLTRSSYHAGEHFEKLKQMRLQNII
>Mature_302_residues
MCSDKNITATALVRPSWLRVKAPFSDEYQSTNELIKSLKLNTVCKEAACPNIGECWSKKHATVMILGSICTRACAFCNVS
TGKPEQVDEYEPYRLSEAVMKLGLKHVVITSVDRDDISDGGASHFAKCITYIRERSPSTSIEVLTPDFLRKHDAWKIVAK
ARPDVYNHNIETVPSLYLKVRPGARYYNSLNLLHQVKIFDSSIFTKSGIMVGLGETKHEVLQVMDDLRAAEVDFLTIGQY
LRPSARHIDVDRYVAPDEFDYYARVAKSKGFLMVSASPLTRSSYHAGEHFEKLKQMRLQNII

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=45.2631578947368, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=45.4918032786885, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=50.5415162454874, Blast_Score=289, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=43.75, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=45.9363957597173, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=43.1095406360424, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34100; Mature: 34100

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSDKNITATALVRPSWLRVKAPFSDEYQSTNELIKSLKLNTVCKEAACPNIGECWSKKH
CCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
ATVMILGSICTRACAFCNVSTGKPEQVDEYEPYRLSEAVMKLGLKHVVITSVDRDDISDG
CEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
GASHFAKCITYIRERSPSTSIEVLTPDFLRKHDAWKIVAKARPDVYNHNIETVPSLYLKV
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCEEEEECCCCCHHCCCCHHCCEEEEEE
RPGARYYNSLNLLHQVKIFDSSIFTKSGIMVGLGETKHEVLQVMDDLRAAEVDFLTIGQY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHH
LRPSARHIDVDRYVAPDEFDYYARVAKSKGFLMVSASPLTRSSYHAGEHFEKLKQMRLQN
HCCCCCEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
II
CC
>Mature Secondary Structure
MCSDKNITATALVRPSWLRVKAPFSDEYQSTNELIKSLKLNTVCKEAACPNIGECWSKKH
CCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
ATVMILGSICTRACAFCNVSTGKPEQVDEYEPYRLSEAVMKLGLKHVVITSVDRDDISDG
CEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
GASHFAKCITYIRERSPSTSIEVLTPDFLRKHDAWKIVAKARPDVYNHNIETVPSLYLKV
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCEEEEECCCCCHHCCCCHHCCEEEEEE
RPGARYYNSLNLLHQVKIFDSSIFTKSGIMVGLGETKHEVLQVMDDLRAAEVDFLTIGQY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHH
LRPSARHIDVDRYVAPDEFDYYARVAKSKGFLMVSASPLTRSSYHAGEHFEKLKQMRLQN
HCCCCCEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
II
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA