Click here to switch to the map view.

The map label for this gene is smpB [H]

Identifier: 189182991

GI number: 189182991

Start: 89257

End: 89718

Strand: Direct

Name: smpB [H]

Synonym: OTT_0084

Alternate gene names: 189182991

Gene position: 89257-89718 (Clockwise)

Preceding gene: 189182990

Following gene: 189182992

Centisome position: 4.44

GC content: 30.3

Gene sequence:

>462_bases
ATGGAGAATTATTGTAAAATAGTTGCTCAAAATAGAAAAGCTAAGTTTAATTACTTTATTGAAGATAAAATGGAAGCAGG
AATAGTTTTACAGGGCAGTGAATTAAAATCTATAAGAAGCGGGAAAGTAAGTATTGAAGATAGTTATGCTGCTGAGAGTA
GCAATGAAATATTTCTCTACAATTCTTATATAGGAGAATATAAACAGGCGAATAGATTTAATCATGTTCCTCGTAGAGTA
AGGAAGCTGTTGCTTCATAGAAAAGAAATACAAAAGATTATTGGCAAACTTAGTGTTCAAGGATGTACATTAGTGGCACT
ATCCATTTACTTTAATAGCAAAAACAAGGTAAAAGTTGAGCTTGGATTAGCTAAGGGAAAAAAACAATATGATAAGCGTT
ATGCTATTAAAGAACAAGAATGGAAAAAACAACAAGCAAGAATAATGAGAAATAAGTTTTAG

Upstream 100 bases:

>100_bases
AATTGTGTACTCCTGAAGTAAGATTACCATTATGCCAGTTAATGCCATCTAGCATCAAATTAGTAAACGAAGTTTTTAGT
GAAGTTGTAATAAGGTAGTT

Downstream 100 bases:

>100_bases
TGGATGAAAAAAGTTTTAAATGATAATTGATCCTGATTACTTAATTGAAAGAAAGTATTATAAAAGCCAATTAAAAAAGT
GGAAGATTCTTACTTTGTTG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MENYCKIVAQNRKAKFNYFIEDKMEAGIVLQGSELKSIRSGKVSIEDSYAAESSNEIFLYNSYIGEYKQANRFNHVPRRV
RKLLLHRKEIQKIIGKLSVQGCTLVALSIYFNSKNKVKVELGLAKGKKQYDKRYAIKEQEWKKQQARIMRNKF

Sequences:

>Translated_153_residues
MENYCKIVAQNRKAKFNYFIEDKMEAGIVLQGSELKSIRSGKVSIEDSYAAESSNEIFLYNSYIGEYKQANRFNHVPRRV
RKLLLHRKEIQKIIGKLSVQGCTLVALSIYFNSKNKVKVELGLAKGKKQYDKRYAIKEQEWKKQQARIMRNKF
>Mature_153_residues
MENYCKIVAQNRKAKFNYFIEDKMEAGIVLQGSELKSIRSGKVSIEDSYAAESSNEIFLYNSYIGEYKQANRFNHVPRRV
RKLLLHRKEIQKIIGKLSVQGCTLVALSIYFNSKNKVKVELGLAKGKKQYDKRYAIKEQEWKKQQARIMRNKF

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=149, Percent_Identity=46.9798657718121, Blast_Score=129, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17932; Mature: 17932

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENYCKIVAQNRKAKFNYFIEDKMEAGIVLQGSELKSIRSGKVSIEDSYAAESSNEIFLY
CCCHHHHHCCCCCCEEEEEEECCCCCCEEEECCHHHHHHCCCEEECCCCCCCCCCEEEEE
NSYIGEYKQANRFNHVPRRVRKLLLHRKEIQKIIGKLSVQGCTLVALSIYFNSKNKVKVE
ECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEE
LGLAKGKKQYDKRYAIKEQEWKKQQARIMRNKF
EECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENYCKIVAQNRKAKFNYFIEDKMEAGIVLQGSELKSIRSGKVSIEDSYAAESSNEIFLY
CCCHHHHHCCCCCCEEEEEEECCCCCCEEEECCHHHHHHCCCEEECCCCCCCCCCEEEEE
NSYIGEYKQANRFNHVPRRVRKLLLHRKEIQKIIGKLSVQGCTLVALSIYFNSKNKVKVE
ECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEE
LGLAKGKKQYDKRYAIKEQEWKKQQARIMRNKF
EECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA