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The map label for this gene is pgk

Identifier: 189182911

GI number: 189182911

Start: 8802

End: 10022

Strand: Direct

Name: pgk

Synonym: OTT_0004

Alternate gene names: 189182911

Gene position: 8802-10022 (Clockwise)

Preceding gene: 189182910

Following gene: 189182912

Centisome position: 0.44

GC content: 32.43

Gene sequence:

>1221_bases
ATGATACAGTTAAGACAACTTTCAGATGTTATAGTAAAAGATAAAGTAGTTTTACTAAGACTGGATCTTAATATACCTCA
AGAAGGTGGCAAAATAACTGATAATACACGTATAGTTCGTACTATACCAACAATAAAATATCTAATTTTACATGGAGCAA
AGGTAGTAATTATCTCACACCTTGGAAACCCAAAAGGTAGAATAGAATTAACATTATCGCTAAGATCAGTTGTGACTGAA
CTTGAAGCATTGTTAAATATTAAGGTGCAATTTTGTCCTGAATCAATTGGATCAACTCCTAAAAACGCTATAATAAAGAT
GAAAGCCGGAGAAGTTTTATTGCTTGAAAATTTAAGATTTAATTCAGGAGAAGAGCTAAACGATGCTACTTTCGTTAACG
AACTTAGTAGCTTAGGAGATATATATGTTAACGATGCTTTTTCTTGCTCTCATAGAAAACATGCATCTATTTGTGGTTTA
CCAGCTAAATTACCTTCAGCAGCTGGGTTTTTATTATTAAGCGAGCTAAAACATTTAACTTCAATTTTTAGTAACGCTAA
TAAGCCATTTACTGTAATTATAGGCGGAGCAAAAATGTCCACAAAATTAGATTTGTTGAATAGTTTGATTACTAAAGCAG
ATTATTTAATCGTTGCTGGTGCTATGGCTAATATTTTTCTAGCTATGAAGCGCTTTAATATTGGAGCATCATTATATAAA
CCAGAACTGGTAAATGTAGCTTCACTAATTTTAAAAAAAGCAACTTCGACAAATTGTAAAATAATACTTCCGTTCGATGC
AGTAATACAAAATTTTAATAGTAACAACATAACTATAATAGAATTAAACTCCTCTTTAGTGATGCAATCTAATGCTAAAA
TTATGGATATTGGTCCTAAAACAATTGCTCAGATAATTAACATAATAAAAATATCTAAAACTATAGTATGGAATGGACCA
GTTGGCGCCTTTGAACAACTCCCTTTTGATCATGGCTCAACCTACCTAAGTAAAGCAATAGCCGAAAAAACTAGAACTGG
CAGCCTTTGTAGTGTTGCTGGTGGCGGAGATACCATATCAGCAATAAAAAAGTCTGGAGTTATTGACGATTTTAGCTATA
TCTCAACTGGAGGCGGCGCTTTTCTTGAATGGTTACAAGGTAAAACATTGCCAGGAGTTGAAGCTTTGATGCAAAAATTA
GATACAGCTAAATACATATAA

Upstream 100 bases:

>100_bases
GTTGCAGAGATGAGAAATGAAAGAAATAATATTACACTACTAGCACAGTAATTCAACTTCCTTGACTATATATGTAAACT
GACACAAGTATGAAATATTT

Downstream 100 bases:

>100_bases
TGTTATAGTAATTATGATCGTTATAGTCTATAATCTTGATGATGCTATAAAAGAACTAAATAGTATTCATGTTCCTGTTA
TTATCACTAATCCACCAGGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIQLRQLSDVIVKDKVVLLRLDLNIPQEGGKITDNTRIVRTIPTIKYLILHGAKVVIISHLGNPKGRIELTLSLRSVVTE
LEALLNIKVQFCPESIGSTPKNAIIKMKAGEVLLLENLRFNSGEELNDATFVNELSSLGDIYVNDAFSCSHRKHASICGL
PAKLPSAAGFLLLSELKHLTSIFSNANKPFTVIIGGAKMSTKLDLLNSLITKADYLIVAGAMANIFLAMKRFNIGASLYK
PELVNVASLILKKATSTNCKIILPFDAVIQNFNSNNITIIELNSSLVMQSNAKIMDIGPKTIAQIINIIKISKTIVWNGP
VGAFEQLPFDHGSTYLSKAIAEKTRTGSLCSVAGGGDTISAIKKSGVIDDFSYISTGGGAFLEWLQGKTLPGVEALMQKL
DTAKYI

Sequences:

>Translated_406_residues
MIQLRQLSDVIVKDKVVLLRLDLNIPQEGGKITDNTRIVRTIPTIKYLILHGAKVVIISHLGNPKGRIELTLSLRSVVTE
LEALLNIKVQFCPESIGSTPKNAIIKMKAGEVLLLENLRFNSGEELNDATFVNELSSLGDIYVNDAFSCSHRKHASICGL
PAKLPSAAGFLLLSELKHLTSIFSNANKPFTVIIGGAKMSTKLDLLNSLITKADYLIVAGAMANIFLAMKRFNIGASLYK
PELVNVASLILKKATSTNCKIILPFDAVIQNFNSNNITIIELNSSLVMQSNAKIMDIGPKTIAQIINIIKISKTIVWNGP
VGAFEQLPFDHGSTYLSKAIAEKTRTGSLCSVAGGGDTISAIKKSGVIDDFSYISTGGGAFLEWLQGKTLPGVEALMQKL
DTAKYI
>Mature_406_residues
MIQLRQLSDVIVKDKVVLLRLDLNIPQEGGKITDNTRIVRTIPTIKYLILHGAKVVIISHLGNPKGRIELTLSLRSVVTE
LEALLNIKVQFCPESIGSTPKNAIIKMKAGEVLLLENLRFNSGEELNDATFVNELSSLGDIYVNDAFSCSHRKHASICGL
PAKLPSAAGFLLLSELKHLTSIFSNANKPFTVIIGGAKMSTKLDLLNSLITKADYLIVAGAMANIFLAMKRFNIGASLYK
PELVNVASLILKKATSTNCKIILPFDAVIQNFNSNNITIIELNSSLVMQSNAKIMDIGPKTIAQIINIIKISKTIVWNGP
VGAFEQLPFDHGSTYLSKAIAEKTRTGSLCSVAGGGDTISAIKKSGVIDDFSYISTGGGAFLEWLQGKTLPGVEALMQKL
DTAKYI

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=37.5609756097561, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=37.3170731707317, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=38.5390428211587, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17508823, Length=399, Percent_Identity=39.3483709273183, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=37.3170731707317, Blast_Score=262, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=37.6811594202899, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17136394, Length=399, Percent_Identity=38.0952380952381, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ORITI (B3CQC3)

Other databases:

- EMBL:   AP008981
- RefSeq:   YP_001936696.1
- GeneID:   6337820
- GenomeReviews:   AP008981_GR
- KEGG:   ott:OTT_0004
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   CLSK2325765
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43938; Mature: 43938

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 119-119 BINDING 152-152 BINDING 202-202 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLRQLSDVIVKDKVVLLRLDLNIPQEGGKITDNTRIVRTIPTIKYLILHGAKVVIISH
CCCHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCEEEEEHHHHHHHHHCCCEEEEEEE
LGNPKGRIELTLSLRSVVTELEALLNIKVQFCPESIGSTPKNAIIKMKAGEVLLLENLRF
CCCCCCEEEEEEEHHHHHHHHHHHHCCEEEECCHHHCCCCCCCEEEEECCCEEEEECCCC
NSGEELNDATFVNELSSLGDIYVNDAFSCSHRKHASICGLPAKLPSAAGFLLLSELKHLT
CCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIFSNANKPFTVIIGGAKMSTKLDLLNSLITKADYLIVAGAMANIFLAMKRFNIGASLYK
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCC
PELVNVASLILKKATSTNCKIILPFDAVIQNFNSNNITIIELNSSLVMQSNAKIMDIGPK
CHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCEEEEEECCCEEEECCCEEEECCHH
TIAQIINIIKISKTIVWNGPVGAFEQLPFDHGSTYLSKAIAEKTRTGSLCSVAGGGDTIS
HHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHH
AIKKSGVIDDFSYISTGGGAFLEWLQGKTLPGVEALMQKLDTAKYI
HHHHCCCCCCCHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQLRQLSDVIVKDKVVLLRLDLNIPQEGGKITDNTRIVRTIPTIKYLILHGAKVVIISH
CCCHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCEEEEEHHHHHHHHHCCCEEEEEEE
LGNPKGRIELTLSLRSVVTELEALLNIKVQFCPESIGSTPKNAIIKMKAGEVLLLENLRF
CCCCCCEEEEEEEHHHHHHHHHHHHCCEEEECCHHHCCCCCCCEEEEECCCEEEEECCCC
NSGEELNDATFVNELSSLGDIYVNDAFSCSHRKHASICGLPAKLPSAAGFLLLSELKHLT
CCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIFSNANKPFTVIIGGAKMSTKLDLLNSLITKADYLIVAGAMANIFLAMKRFNIGASLYK
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCC
PELVNVASLILKKATSTNCKIILPFDAVIQNFNSNNITIIELNSSLVMQSNAKIMDIGPK
CHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCEEEEEECCCEEEECCCEEEECCHH
TIAQIINIIKISKTIVWNGPVGAFEQLPFDHGSTYLSKAIAEKTRTGSLCSVAGGGDTIS
HHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHH
AIKKSGVIDDFSYISTGGGAFLEWLQGKTLPGVEALMQKLDTAKYI
HHHHCCCCCCCHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA