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The map label for this gene is gatB

Identifier: 189026244

GI number: 189026244

Start: 1114864

End: 1116393

Strand: Direct

Name: gatB

Synonym: TPASS_1021

Alternate gene names: 189026244

Gene position: 1114864-1116393 (Clockwise)

Preceding gene: 189026243

Following gene: 189026249

Centisome position: 97.84

GC content: 52.16

Gene sequence:

>1530_bases
ATGTCTGACCTCCAAACAGGCACAGTTCCCTCCATTGCAGGCGCCACAGATGACACACATGCCGCACCCTTTTTCTACGA
GGTAATTATTGGCTGTGAAATTCATTGTCAGCTTCTAACAAAGACCAAAGCTTTCTGTGCCTGTGCAAATCGCTCAGGAG
GAATGCCGAATAGCCGTGTGTGTCCTGTGTGTCTTGGGTTGCCAGGAGCGTTGCCCGTTGTGAGTGAAGAGTACGTGCGG
CTCGGGGTGCGCGCCGGACTTGCGTTGGGGTGCACTATCCAGCTTTGGTCCGCTTTTGATCGCAAGCACTATTTTTATCC
AGATCTCCCAAAGGGTTATCAAATTACCCAGTACGACGCTCCCTTGTGTACGGATGGTGCAGTGGATGTACAGGGAGTTG
ACATGCCCGTGCAGCGGCGTGTCCGTATTGAACGGATACATTTGGAGGAGGACGCAGGCAAAAGCCTGCATGCTGCAGAC
GCTTACAGCTATATTGATTTCAATCGTTGTGGGGTGCCGCTCATTGAAATTGTATCTAGGCCGGATCTGCGCTCTGCAGA
GGAGGCCGCATGTTTTATGCAGACGATCCGCGAGATTCTCACCTTTATCGAGGTAACGGATGGTAATTTAGAAGAAGGCG
CACTGCGATGCGACGCGAATGTTAATGTGAGGATTCTGTACAAAGGGCAAGAACACCACACTCCCATTTCTGAAATCAAA
AATATGAACTCGTATCGTATGGTGCGGGACGCGTGTACGTATGAGGTACAGCGTCAATTGCAGGAGTTTTGGCAAAAGGG
TCCTGCGAGCAAAGAAGAGATGCAGAGAAAACGCACGATGGGCTGGGATCCGGTCGAAGGGGTTACGCTTTTACAGCGTA
CAAAGCACTCACTGCGCGATTATCGTTTCATGCGCGATCCAGACTTACCTGACCTGCACTTGACCCCTGCATATGTCCAG
CATCTCTCTTACACAGTCGGGGAACTTCCGGCAGCGCGGCGTGCACGTTTCAAACTTGACCTTGGCTTGTCGGCGTTTGC
AGCCCAAACGCTTACCGGCAGCCGCATGCTCGCAGACTGGTTTGAGAAGGCAGCGCATGCGTCTAAGAATGCGCGACGAG
TGGCAAACTGGATTCTGTCGGAGGTTCTTGCGGTAGTAAACGAGAAGAATATCTGCATTGCAGAGCTCAATCTGAGTCCT
GAAGCAATTGCCGAACTAATGGATGCAGTTGAAGATCAGCGCATTACCGGAAAACAAGCAAAGGATATATTTGCACAAAT
GCTTGCCACCGGTGCGCGAGCGCAGGACATTATCTCCGCACAGGGTCTGGCACAACTTTCAGATGAGGAAGAAATCGCAA
CGTTAGTGCAGACGGTGTTTCAAGAACATCCAAAGGCACTGCGTGATTGGCAACACGGTAAGACAAACGTGGCTGCCTGG
CTCATGGGGCAAGTAATGAAGCGTTCCCGCGGGCGCGCACACCCTGCGCGAGTGGCGACGCTCGTCCACCAAGCACTCTC
TCAGCTGTAA

Upstream 100 bases:

>100_bases
ACATCAGGCACTGGCCTACCCATCGGAATACAGATTATCGGTTCTCAGTGGCAGGAGTGTGCCGTTCTCCGGCTAGCAAA
ACGTTGGGAGGAGGCACCTC

Downstream 100 bases:

>100_bases
CAGCTGGAAAAACTCCACGGAAGAGCGGCGGTCTCTCTTTCAGCATACGCCCGGCCCGGCTCACGCCAGGAGAAGAAAGA
CGCACCAAAGGGCTACTACG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRVCPVCLGLPGALPVVSEEYVR
LGVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDAPLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAAD
AYSYIDFNRCGVPLIEIVSRPDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIK
NMNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRDYRFMRDPDLPDLHLTPAYVQ
HLSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADWFEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSP
EAIAELMDAVEDQRITGKQAKDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAW
LMGQVMKRSRGRAHPARVATLVHQALSQL

Sequences:

>Translated_509_residues
MSDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRVCPVCLGLPGALPVVSEEYVR
LGVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDAPLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAAD
AYSYIDFNRCGVPLIEIVSRPDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIK
NMNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRDYRFMRDPDLPDLHLTPAYVQ
HLSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADWFEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSP
EAIAELMDAVEDQRITGKQAKDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAW
LMGQVMKRSRGRAHPARVATLVHQALSQL
>Mature_508_residues
SDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRVCPVCLGLPGALPVVSEEYVRL
GVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDAPLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAADA
YSYIDFNRCGVPLIEIVSRPDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIKN
MNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRDYRFMRDPDLPDLHLTPAYVQH
LSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADWFEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSPE
AIAELMDAVEDQRITGKQAKDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAWL
MGQVMKRSRGRAHPARVATLVHQALSQL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=509, Percent_Identity=32.4165029469548, Blast_Score=252, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI115534094, Length=335, Percent_Identity=36.7164179104478, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=29.1746641074856, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550808, Length=507, Percent_Identity=31.7554240631164, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_TREPA (O83984)

Other databases:

- EMBL:   AE000520
- PIR:   A71254
- RefSeq:   NP_219458.1
- ProteinModelPortal:   O83984
- SMR:   O83984
- GeneID:   2610877
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP1021
- NMPDR:   fig|243276.1.peg.1016
- TIGR:   TP_1021
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   TPAL243276:TP_1021-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 56866; Mature: 56735

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHEEHHHHHHHHHHHCCCCCCCCCCCCC
CPVCLGLPGALPVVSEEYVRLGVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDA
CHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
PLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAADAYSYIDFNRCGVPLIEIVSR
CCCCCCCCEECCCCCCHHHHHHHHEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCC
PDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHH
NMNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YRFMRDPDLPDLHLTPAYVQHLSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADW
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCEEEECCHHHHHHHHHCCHHHHHHH
FEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSPEAIAELMDAVEDQRITGKQA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
KDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAW
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHH
LMGQVMKRSRGRAHPARVATLVHQALSQL
HHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHEEHHHHHHHHHHHCCCCCCCCCCCCC
CPVCLGLPGALPVVSEEYVRLGVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDA
CHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
PLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAADAYSYIDFNRCGVPLIEIVSR
CCCCCCCCEECCCCCCHHHHHHHHEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCC
PDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHH
NMNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YRFMRDPDLPDLHLTPAYVQHLSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADW
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCEEEECCHHHHHHHHHCCHHHHHHH
FEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSPEAIAELMDAVEDQRITGKQA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
KDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAW
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHH
LMGQVMKRSRGRAHPARVATLVHQALSQL
HHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876