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The map label for this gene is gatA

Identifier: 189026243

GI number: 189026243

Start: 1113351

End: 1114871

Strand: Direct

Name: gatA

Synonym: TPASS_1020

Alternate gene names: 189026243

Gene position: 1113351-1114871 (Clockwise)

Preceding gene: 189026242

Following gene: 189026244

Centisome position: 97.71

GC content: 55.69

Gene sequence:

>1521_bases
ATGGACGCGCATGCTATTACCTGTGCAAGCTGGAATATGTTAAAGGCTCAGCTTGAAGCCGGTGCAATCAGCTCTTTGCA
GATTGTGCGTGCGTTTCGCAACGTATACGAGGAAGACACACGCAGCGCGTCCCCGCTTGGGGCTTTGGTCGAGTTTTTCT
CTGATGCGGAGGAGCACGCGCGTACGGCAGACAATCTCCGTGCCTCGTGTGCCCAGAGTACTAAAACAGCTGGAGCAAAC
GGGGGGAGTGTCTCAGGTAAGCCTTTGTTAGGTCTACCCTTTGCTGTCAAGGACAATATTTCAGTGAAAGGAAAGCACTG
CACGTGTGGCAGTAAACTCCTTGCAGACTATAGGGCTCCGTACGATGCCACCGTTGTTGCGCGCCTGCGCGCCGCAGGTG
CTATCCCGCTCGGGAGAACGAACATGGATGAGTTTGCTATGGGCTCTTCCACCGAGTATTCTGTTTATGGGCCGACGCGT
AATCCTCGGGATCGGAGCCGCACCAGCGGGGGAAGTTCCGGCGGTTCGGCTGCCGCCGTCGCAGGCGGTCAGGCACCGTT
TGCACTCGGTACCGAAACGGGAGGCTCGGTACGCCTGCCAGCTGCTTACTGCGGCCTCTATGGCCTGAAGCCGACCTATG
GTCTCTTGAGTCGATATGGGGTGGTTGCCTTTGGCTCCTCTCTAGACCAAATCGGCTTTTTTGCTACCTGCATTGACGAT
ATTGCCCTCGCCCTCTCCGTCACCTCAGGGAAAGACCTGTACGACAGCACGAGCACTTGCCCCCCTCCTGCGACGGGGCG
ACACGCTGTGTCTCACCATCTTGCCCCTTTTTCTGCCCACGAGTGCTCTATCCTGCGTGCTGCTGTTCCCCGCGAATTAG
TAGATGCTCCTGGCGTGCATCCTGACGTGTCTGCGCAATTTCAACGCTTCCTCACCTGGCTGCGTGCCCAAAACGTACAG
GTAGAAGAAGTGACGCTTCCTGCACTACAGGCGGCAGTGCCTGTATATTATCTTGTCGCGACAGCTGAAGCCGCCAGCAA
TCTTGCGCGTTTTGACGGTATTCGCTACGGGCAGAGGGGAGACACTGATGCTCTTTTGGAAAATTACTACCGCGCCGTCC
GTACCTCAGGCTTTGGACCCGAAGTACAGCGAAGGATCATTGTGGGGAATTATGTTCTTTCACGCCATTTCTCCGGTGAT
TATTACCGAACGAGTGTGCGCGTACGTTCGCGTATAGAACAAGAATGTACGCAGCTCCTCTGTTCCTACCACTTTATTGT
TTGTCCTACTGCCGCTACCGGTGCCTTCCCGCTTGGAGAACGCATACATGACCCGCTGGCCATGTATTGCTCGGATTTAT
TCACCACCTTCGTTAACCTTGCCCGCCTACCGGCGCTATCAGTACCAGTGGGAACATCAGGCACTGGCCTACCCATCGGA
ATACAGATTATCGGTTCTCAGTGGCAGGAGTGTGCCGTTCTCCGGCTAGCAAAACGTTGGGAGGAGGCACCTCATGTCTG
A

Upstream 100 bases:

>100_bases
CGCATTACCTGGTCTTTCCGTTCACGATCTCAAGCGGTTGTCCACAGAGTTTGCTGACGGTTACTTTCGCGCACCGCGCG
CGCTCGATGGTAGCGCATAA

Downstream 100 bases:

>100_bases
CCTCCAAACAGGCACAGTTCCCTCCATTGCAGGCGCCACAGATGACACACATGCCGCACCCTTTTTCTACGAGGTAATTA
TTGGCTGTGAAATTCATTGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MDAHAITCASWNMLKAQLEAGAISSLQIVRAFRNVYEEDTRSASPLGALVEFFSDAEEHARTADNLRASCAQSTKTAGAN
GGSVSGKPLLGLPFAVKDNISVKGKHCTCGSKLLADYRAPYDATVVARLRAAGAIPLGRTNMDEFAMGSSTEYSVYGPTR
NPRDRSRTSGGSSGGSAAAVAGGQAPFALGTETGGSVRLPAAYCGLYGLKPTYGLLSRYGVVAFGSSLDQIGFFATCIDD
IALALSVTSGKDLYDSTSTCPPPATGRHAVSHHLAPFSAHECSILRAAVPRELVDAPGVHPDVSAQFQRFLTWLRAQNVQ
VEEVTLPALQAAVPVYYLVATAEAASNLARFDGIRYGQRGDTDALLENYYRAVRTSGFGPEVQRRIIVGNYVLSRHFSGD
YYRTSVRVRSRIEQECTQLLCSYHFIVCPTAATGAFPLGERIHDPLAMYCSDLFTTFVNLARLPALSVPVGTSGTGLPIG
IQIIGSQWQECAVLRLAKRWEEAPHV

Sequences:

>Translated_506_residues
MDAHAITCASWNMLKAQLEAGAISSLQIVRAFRNVYEEDTRSASPLGALVEFFSDAEEHARTADNLRASCAQSTKTAGAN
GGSVSGKPLLGLPFAVKDNISVKGKHCTCGSKLLADYRAPYDATVVARLRAAGAIPLGRTNMDEFAMGSSTEYSVYGPTR
NPRDRSRTSGGSSGGSAAAVAGGQAPFALGTETGGSVRLPAAYCGLYGLKPTYGLLSRYGVVAFGSSLDQIGFFATCIDD
IALALSVTSGKDLYDSTSTCPPPATGRHAVSHHLAPFSAHECSILRAAVPRELVDAPGVHPDVSAQFQRFLTWLRAQNVQ
VEEVTLPALQAAVPVYYLVATAEAASNLARFDGIRYGQRGDTDALLENYYRAVRTSGFGPEVQRRIIVGNYVLSRHFSGD
YYRTSVRVRSRIEQECTQLLCSYHFIVCPTAATGAFPLGERIHDPLAMYCSDLFTTFVNLARLPALSVPVGTSGTGLPIG
IQIIGSQWQECAVLRLAKRWEEAPHV
>Mature_506_residues
MDAHAITCASWNMLKAQLEAGAISSLQIVRAFRNVYEEDTRSASPLGALVEFFSDAEEHARTADNLRASCAQSTKTAGAN
GGSVSGKPLLGLPFAVKDNISVKGKHCTCGSKLLADYRAPYDATVVARLRAAGAIPLGRTNMDEFAMGSSTEYSVYGPTR
NPRDRSRTSGGSSGGSAAAVAGGQAPFALGTETGGSVRLPAAYCGLYGLKPTYGLLSRYGVVAFGSSLDQIGFFATCIDD
IALALSVTSGKDLYDSTSTCPPPATGRHAVSHHLAPFSAHECSILRAAVPRELVDAPGVHPDVSAQFQRFLTWLRAQNVQ
VEEVTLPALQAAVPVYYLVATAEAASNLARFDGIRYGQRGDTDALLENYYRAVRTSGFGPEVQRRIIVGNYVLSRHFSGD
YYRTSVRVRSRIEQECTQLLCSYHFIVCPTAATGAFPLGERIHDPLAMYCSDLFTTFVNLARLPALSVPVGTSGTGLPIG
IQIIGSQWQECAVLRLAKRWEEAPHV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=465, Percent_Identity=33.9784946236559, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=23.155737704918, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI166795287, Length=178, Percent_Identity=34.2696629213483, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=34.9425287356322, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=24.7464503042596, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=175, Percent_Identity=40, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=206, Percent_Identity=32.0388349514563, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=207, Percent_Identity=29.4685990338164, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=218, Percent_Identity=30.2752293577982, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=187, Percent_Identity=34.7593582887701, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=187, Percent_Identity=34.7593582887701, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=33.5648148148148, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319685, Length=418, Percent_Identity=33.9712918660287, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=129, Percent_Identity=35.6589147286822, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=432, Percent_Identity=37.962962962963, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=28.6836935166994, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652985, Length=513, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922090, Length=513, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652981, Length=513, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652983, Length=513, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=27.7131782945736, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=38.5416666666667, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=38.5416666666667, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=38.5416666666667, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=208, Percent_Identity=33.1730769230769, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=125, Percent_Identity=43.2, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648439, Length=125, Percent_Identity=43.2, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_TREPA (O83983)

Other databases:

- EMBL:   AE000520
- PIR:   H71253
- RefSeq:   NP_219457.1
- ProteinModelPortal:   O83983
- SMR:   O83983
- IntAct:   O83983
- GeneID:   2610872
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP1020
- NMPDR:   fig|243276.1.peg.1015
- TIGR:   TP_1020
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   TPAL243276:TP_1020-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 54103; Mature: 54103

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 97-97 ACT_SITE 172-172 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAHAITCASWNMLKAQLEAGAISSLQIVRAFRNVYEEDTRSASPLGALVEFFSDAEEHA
CCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RTADNLRASCAQSTKTAGANGGSVSGKPLLGLPFAVKDNISVKGKHCTCGSKLLADYRAP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEECCCCEECCCCCCCHHHHHHHHCCC
YDATVVARLRAAGAIPLGRTNMDEFAMGSSTEYSVYGPTRNPRDRSRTSGGSSGGSAAAV
CCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEE
AGGQAPFALGTETGGSVRLPAAYCGLYGLKPTYGLLSRYGVVAFGSSLDQIGFFATCIDD
CCCCCCEEEECCCCCCEECCHHHHCCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHH
IALALSVTSGKDLYDSTSTCPPPATGRHAVSHHLAPFSAHECSILRAAVPRELVDAPGVH
HHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCCCC
PDVSAQFQRFLTWLRAQNVQVEEVTLPALQAAVPVYYLVATAEAASNLARFDGIRYGQRG
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCC
DTDALLENYYRAVRTSGFGPEVQRRIIVGNYVLSRHFSGDYYRTSVRVRSRIEQECTQLL
CHHHHHHHHHHHHHCCCCCHHHHHEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
CSYHFIVCPTAATGAFPLGERIHDPLAMYCSDLFTTFVNLARLPALSVPVGTSGTGLPIG
HHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
IQIIGSQWQECAVLRLAKRWEEAPHV
EEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDAHAITCASWNMLKAQLEAGAISSLQIVRAFRNVYEEDTRSASPLGALVEFFSDAEEHA
CCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RTADNLRASCAQSTKTAGANGGSVSGKPLLGLPFAVKDNISVKGKHCTCGSKLLADYRAP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEECCCCEECCCCCCCHHHHHHHHCCC
YDATVVARLRAAGAIPLGRTNMDEFAMGSSTEYSVYGPTRNPRDRSRTSGGSSGGSAAAV
CCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEE
AGGQAPFALGTETGGSVRLPAAYCGLYGLKPTYGLLSRYGVVAFGSSLDQIGFFATCIDD
CCCCCCEEEECCCCCCEECCHHHHCCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHH
IALALSVTSGKDLYDSTSTCPPPATGRHAVSHHLAPFSAHECSILRAAVPRELVDAPGVH
HHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCCCC
PDVSAQFQRFLTWLRAQNVQVEEVTLPALQAAVPVYYLVATAEAASNLARFDGIRYGQRG
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCC
DTDALLENYYRAVRTSGFGPEVQRRIIVGNYVLSRHFSGDYYRTSVRVRSRIEQECTQLL
CHHHHHHHHHHHHHCCCCCHHHHHEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
CSYHFIVCPTAATGAFPLGERIHDPLAMYCSDLFTTFVNLARLPALSVPVGTSGTGLPIG
HHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
IQIIGSQWQECAVLRLAKRWEEAPHV
EEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876