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The map label for this gene is gap

Identifier: 189026068

GI number: 189026068

Start: 918200

End: 919252

Strand: Direct

Name: gap

Synonym: TPASS_0844

Alternate gene names: 189026068

Gene position: 918200-919252 (Clockwise)

Preceding gene: 189026064

Following gene: 189026075

Centisome position: 80.58

GC content: 54.89

Gene sequence:

>1053_bases
ATGAGAGTTGCAATCAATGGTTTTGGGCGTATCGGGCGTCTCGTACTCCAGGCCATGGCGGAGCAGAAGCTGTTGGGGAA
AGAGTTCGACGTGGCGGCAGTTGTAGACCTTTCCACCGATGCGCGCTACTTTGCCTATCAGCTAAAGTATGACTCCGTGC
AGGGCAAAATGGGTTCTAGTCTCAGTGCTCCTGCCGAGGATATCCTCGAGGTGGGCGGGCATCGGATCAAGTGTGTCTGC
GGTCGCGGCTTAAAGCCTTCCCAACTTCCCTGGAAGGATCTTGGTATTGAGGTGGTTATTGAGGCCACCGGCATTTACGC
GAATGAGTCTTCATACGGTCACCTTGAAGCAGGAGCAAAGCGAGTCATCATCAGTGCTCCGGCTAAGAGCTCGGATGCGT
CTAAGCCGGTGAAGACTATTGTAATGGGGGTGAACGAGCATGAGTTCGATCCTGCTGAGCACAAGGTCGTCTCCAACGCG
AGCTGCACTACCAACTGTCTCGCGCCGGTAGTGCACGTCTTTCTCAAAGAGGGAGTCGGTATTGAAACGGGACTGATGAC
GACTATCCACTCCTATACCGCAACGCAAAAAACTGTAGATGGGGTTTCTTTGAAGGACTGGCGCGGAGGCCGCGCTGCAG
CGGTTAACATTATCCCTTCCACCACTGGAGCTGCGAAAGCGGTAGGCGAAGTGCTGCCGTCTACGAGAGGTAAGTTGACG
GGGATGGCCTTCCGTGTCCCGACACCAACCGGTTCCGTGGTGGATCTAACCTTCCGCACGGAAAAAGAGACGAGCGTTGC
GGACCTCAACGCGATGCTCAAGAAGGCGTCGGAGTCCTATCTGCGGGGTGTCCTGCAGTATTGCGACGAGGATATTGTAT
CCGCGGACGTCATCCACAACCAGTACTCCTCCATCTACGACAGCAGGGCGACGCTGCAGAACAACCTCCCGAACGAGAAG
CGCTTCTTCAAGGTAGTGTCCTGGTATGACAACGAGTGGGGTTACTCAAACCGTGTGGTGGATTTGCTTAAGTTCATCTC
GCAGAAGCGGTAG

Upstream 100 bases:

>100_bases
AAATGCAAGGGAGCCCTACAGCAAAGAGGGATTGACGTGCCGATCTTTTTTCAGATAGCGTTGCGCGGGATGTGTGCTCT
TTTTAGAGGAGGTTGGGCAA

Downstream 100 bases:

>100_bases
AAGACACTAGGAGTCCACCCGCCCGGCCTTGGACTCTTAGTGTCTTGCGTGCGGTCACCGGCGGCCGGTTTGGCTTTTCC
CTTGCGCGGAGGGCCAGTCG

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRVAINGFGRIGRLVLQAMAEQKLLGKEFDVAAVVDLSTDARYFAYQLKYDSVQGKMGSSLSAPAEDILEVGGHRIKCVC
GRGLKPSQLPWKDLGIEVVIEATGIYANESSYGHLEAGAKRVIISAPAKSSDASKPVKTIVMGVNEHEFDPAEHKVVSNA
SCTTNCLAPVVHVFLKEGVGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAVNIIPSTTGAAKAVGEVLPSTRGKLT
GMAFRVPTPTGSVVDLTFRTEKETSVADLNAMLKKASESYLRGVLQYCDEDIVSADVIHNQYSSIYDSRATLQNNLPNEK
RFFKVVSWYDNEWGYSNRVVDLLKFISQKR

Sequences:

>Translated_350_residues
MRVAINGFGRIGRLVLQAMAEQKLLGKEFDVAAVVDLSTDARYFAYQLKYDSVQGKMGSSLSAPAEDILEVGGHRIKCVC
GRGLKPSQLPWKDLGIEVVIEATGIYANESSYGHLEAGAKRVIISAPAKSSDASKPVKTIVMGVNEHEFDPAEHKVVSNA
SCTTNCLAPVVHVFLKEGVGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAVNIIPSTTGAAKAVGEVLPSTRGKLT
GMAFRVPTPTGSVVDLTFRTEKETSVADLNAMLKKASESYLRGVLQYCDEDIVSADVIHNQYSSIYDSRATLQNNLPNEK
RFFKVVSWYDNEWGYSNRVVDLLKFISQKR
>Mature_350_residues
MRVAINGFGRIGRLVLQAMAEQKLLGKEFDVAAVVDLSTDARYFAYQLKYDSVQGKMGSSLSAPAEDILEVGGHRIKCVC
GRGLKPSQLPWKDLGIEVVIEATGIYANESSYGHLEAGAKRVIISAPAKSSDASKPVKTIVMGVNEHEFDPAEHKVVSNA
SCTTNCLAPVVHVFLKEGVGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAVNIIPSTTGAAKAVGEVLPSTRGKLT
GMAFRVPTPTGSVVDLTFRTEKETSVADLNAMLKKASESYLRGVLQYCDEDIVSADVIHNQYSSIYDSRATLQNNLPNEK
RFFKVVSWYDNEWGYSNRVVDLLKFISQKR

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7657116, Length=347, Percent_Identity=48.4149855907781, Blast_Score=307, Evalue=9e-84,
Organism=Homo sapiens, GI7669492, Length=351, Percent_Identity=47.5783475783476, Blast_Score=298, Evalue=4e-81,
Organism=Escherichia coli, GI1788079, Length=349, Percent_Identity=47.2779369627507, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1789295, Length=342, Percent_Identity=38.0116959064327, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32566163, Length=352, Percent_Identity=47.4431818181818, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17568413, Length=352, Percent_Identity=47.4431818181818, Blast_Score=304, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17534677, Length=354, Percent_Identity=48.0225988700565, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17534679, Length=354, Percent_Identity=47.7401129943503, Blast_Score=302, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322468, Length=349, Percent_Identity=49.5702005730659, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6322409, Length=349, Percent_Identity=48.1375358166189, Blast_Score=315, Evalue=6e-87,
Organism=Saccharomyces cerevisiae, GI6321631, Length=349, Percent_Identity=48.1375358166189, Blast_Score=310, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17933600, Length=349, Percent_Identity=47.8510028653295, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI18110149, Length=349, Percent_Identity=47.8510028653295, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI85725000, Length=349, Percent_Identity=48.1375358166189, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI22023983, Length=349, Percent_Identity=48.1375358166189, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI19922412, Length=344, Percent_Identity=44.1860465116279, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_TREPA (O83816)

Other databases:

- EMBL:   AE000520
- PIR:   D71273
- RefSeq:   NP_219280.1
- ProteinModelPortal:   O83816
- SMR:   O83816
- IntAct:   O83816
- GeneID:   2611009
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0844
- NMPDR:   fig|243276.1.peg.839
- TIGR:   TP_0844
- HOGENOM:   HBG571736
- OMA:   KEICTEV
- ProtClustDB:   CLSK793410
- BioCyc:   TPAL243276:TP_0844-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 38084; Mature: 38084

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 162-162 BINDING 36-36 BINDING 193-193 BINDING 245-245 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAINGFGRIGRLVLQAMAEQKLLGKEFDVAAVVDLSTDARYFAYQLKYDSVQGKMGSS
CEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCC
LSAPAEDILEVGGHRIKCVCGRGLKPSQLPWKDLGIEVVIEATGIYANESSYGHLEAGAK
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCEEEEEEECCEEECCCCCCCCCCCCC
RVIISAPAKSSDASKPVKTIVMGVNEHEFDPAEHKVVSNASCTTNCLAPVVHVFLKEGVG
EEEEECCCCCCCCCCCHHHEEECCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHCCCC
IETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAVNIIPSTTGAAKAVGEVLPSTRGKLT
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCC
GMAFRVPTPTGSVVDLTFRTEKETSVADLNAMLKKASESYLRGVLQYCDEDIVSADVIHN
EEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QYSSIYDSRATLQNNLPNEKRFFKVVSWYDNEWGYSNRVVDLLKFISQKR
HHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAINGFGRIGRLVLQAMAEQKLLGKEFDVAAVVDLSTDARYFAYQLKYDSVQGKMGSS
CEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCC
LSAPAEDILEVGGHRIKCVCGRGLKPSQLPWKDLGIEVVIEATGIYANESSYGHLEAGAK
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCEEEEEEECCEEECCCCCCCCCCCCC
RVIISAPAKSSDASKPVKTIVMGVNEHEFDPAEHKVVSNASCTTNCLAPVVHVFLKEGVG
EEEEECCCCCCCCCCCHHHEEECCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHCCCC
IETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAVNIIPSTTGAAKAVGEVLPSTRGKLT
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCC
GMAFRVPTPTGSVVDLTFRTEKETSVADLNAMLKKASESYLRGVLQYCDEDIVSADVIHN
EEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QYSSIYDSRATLQNNLPNEKRFFKVVSWYDNEWGYSNRVVDLLKFISQKR
HHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876