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The map label for this gene is dam

Identifier: 189026035

GI number: 189026035

Start: 880108

End: 881019

Strand: Direct

Name: dam

Synonym: TPASS_0810

Alternate gene names: 189026035

Gene position: 880108-881019 (Clockwise)

Preceding gene: 189026034

Following gene: 189026038

Centisome position: 77.24

GC content: 55.04

Gene sequence:

>912_bases
TTGTCCCGCTCTGACACCGCCCGCCCCTTCGTAAAATGGGCAGGAGGAAAGCGCGCCCTCGCCCCAACCCTTTTTGCGCA
TATGCCACAGACATTCGGCTCCTACTTTGAGCCTTTCGTGGGAGGGGGAGCGCTCTTTTGGCACTTGTGCGCGTGTACTC
GGGTGCGCCTACACGACATCTATCTATCTGACATAAATTGGCCACTGCTGTGTGCGTATGCAGCCGTTCGTGACCGTGTA
GAAGAACTTATCGTCCGGGTTGGACAGCACATCGCCTGCCACACCCCTACCTATTACCGTCTTGCGCGGCGTAAATTCGC
CGTATGCGAGCATCCGCTCGAGGTTGCCGCGCTTTTCCTGTACCTGAATCGGAGCTGCTATAACGGACTGTACCGTGTCA
ATAAAGCAGGTCAATTCAATGTGCCTCTCGGACGCGCTGCACCTGCGTCTCCTTTTCTAAATACCACCGCGCCTACCCCT
CGCAGTACACAGCCTGCGGCGCAGGTCGGACACCTTGCAATACGCATTGATGAGGAGAATTTACGCAGCTGCGCGCGTGC
GCTAGCAAACACCACTCTTAACTGCCAACACTTTTCTTGCATTCAACCTGCACGAGGAGATTTTGTGTATCTCGATCCAC
CGTACCTTGGCACCTTCAGTGCCTATGATAAAACCGGTTTTGATAGAGCAGCGCACGAATCGCTTGCTGCGTTTTGCATG
CACCTAGACGCGCGGGGAGTTCTTTTTATGCTCTCAAACAGCGATTGCCCTGAGGTACGCGCATGGTATCGTCCATTCCG
TGTGCAACAACTCAACGCCCCTCGGTGTATCGCACGATCCGCTCACGCAAGGGGAAAAAGGTGCGAAGTGCTTATCACCA
ATTACCCCTGCGCTGACACGGCTACACCGTAG

Upstream 100 bases:

>100_bases
CAAAAAAAGCGCAGAACAGTGCGCCGCTCGCCTTGCATGGGAACAATTATCCGGCACCCTCCGGGAGTAGCGCGTATGCT
GCCCTGTAAGGATACTCTCC

Downstream 100 bases:

>100_bases
CTTTCTGCACTCTCCTGGCCGTATCGCATCGCGTATTGCGGCGTTTAATGCCACTACAGAAGTTTTACGGTCATAAAAAC
CATCCGTGGGGACCCGCGTG

Product: DNA adenine methyltransferase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.TpaI

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDIYLSDINWPLLCAYAAVRDRV
EELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFLYLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTP
RSTQPAAQVGHLAIRIDEENLRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCM
HLDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADTATP

Sequences:

>Translated_303_residues
MSRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDIYLSDINWPLLCAYAAVRDRV
EELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFLYLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTP
RSTQPAAQVGHLAIRIDEENLRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCM
HLDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADTATP
>Mature_302_residues
SRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDIYLSDINWPLLCAYAAVRDRVE
ELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFLYLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTPR
STQPAAQVGHLAIRIDEENLRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCMH
LDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADTATP

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789789, Length=294, Percent_Identity=29.9319727891156, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMA_TREPA (O33844)

Other databases:

- EMBL:   AF006263
- EMBL:   AE000520
- PIR:   H71277
- RefSeq:   NP_219247.1
- ProteinModelPortal:   O33844
- SMR:   O33844
- IntAct:   O33844
- REBASE:   3131
- GeneID:   2610874
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0810
- NMPDR:   fig|243276.1.peg.806
- TIGR:   TP_0810
- HOGENOM:   HBG586381
- OMA:   KARRTIS
- ProtClustDB:   CLSK2300306
- BioCyc:   TPAL243276:TP_0810-MONOMER
- BRENDA:   2.1.1.72
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327
- Gene3D:   G3DSA:1.10.1020.10
- PRINTS:   PR00505
- TIGRFAMs:   TIGR00571

Pfam domain/function: PF02086 MethyltransfD12

EC number: =2.1.1.72

Molecular weight: Translated: 33919; Mature: 33788

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 13-13 BINDING 17-17 BINDING 64-64 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDI
CCCCCCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEE
YLSDINWPLLCAYAAVRDRVEELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFL
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
YLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTPRSTQPAAQVGHLAIRIDEEN
HHHHHHHHHHEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEEECHHH
LRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCM
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADT
HHCCCCEEEEECCCCCHHHHHHHCCHHEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCC
ATP
CCC
>Mature Secondary Structure 
SRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDI
CCCCCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEE
YLSDINWPLLCAYAAVRDRVEELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFL
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
YLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTPRSTQPAAQVGHLAIRIDEEN
HHHHHHHHHHEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEEECHHH
LRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCM
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADT
HHCCCCEEEEECCCCCHHHHHHHCCHHEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCC
ATP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9345771; 9665876